Leptomycin B

Catalog No.S7580 Synonyms: LMB, CI 940, Elactocin, Mantuamycin, NSC 364372

For research use only.

Leptomycin B (LMB, CI 940, Elactocin, Mantuamycin, NSC 364372) is a potent antifungal antibiotic isolated from Streptomyces and acts as a specific inhibitor of the nuclear export factor CRM1. Leptomycin B rapidly induces cytotoxic effects in cancer cell lines via covalent inhibition of CRM1 with IC50 values of 0.1 nM–10 nM.

Leptomycin B Chemical Structure

CAS No. 87081-35-4

Purity & Quality Control

Choose Selective CRM1 Inhibitors

Biological Activity

Description Leptomycin B (LMB, CI 940, Elactocin, Mantuamycin, NSC 364372) is a potent antifungal antibiotic isolated from Streptomyces and acts as a specific inhibitor of the nuclear export factor CRM1. Leptomycin B rapidly induces cytotoxic effects in cancer cell lines via covalent inhibition of CRM1 with IC50 values of 0.1 nM–10 nM.
Targets
CRM1 [1]
In vitro

Leptomycin B (LMB) is a potent anti-fungal antibiotic from <i>Streptomyces sp</i>., also can inhibit the expression of COX-2 mRNA and protein induced by serum withdrawal in MDA-MB-231 cells.<sup><a class="sref" href="#s_ref">[1]</a></sup><sup><a class="sref" href="#s_ref">[2]</a></sup>

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: MDA-MB-231 Cells
  • Concentrations: 2.5, 5, 10, and 20 ng/ml
  • Incubation Time: 24 h
  • Method:

    MDA-MB-231 cells were grown in culture medium containing 10% FBS without LMB (none or vehicle (v)) or in culture medium containing 0% FBS along with LMB in different concentrations (0, 2.5, 5, 10, and 20 ng/ml) for 24 h. Total RNA was isolated and analyzed for the COX-2 mRNA expression by Northern blot analysis, COX-2 protein expression by Western blot analysis.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 540.73
Formula

C33H48O6

CAS No. 87081-35-4
Storage 3 years -20°C powder
2 years -80°C in solvent

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03804879 Completed Drug: Nidufexor|Other: Placebo|Drug: Standard of Care (SoC) Diabetic Nephropathy Novartis Pharmaceuticals|Novartis December 17 2018 Phase 2
NCT03436732 Terminated Drug: LMB-100|Drug: SEL-110 Mesothelioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) February 28 2018 Phase 1
NCT02913105 Terminated Drug: LMB763|Drug: Placebo Non-alcoholic Steatohepatitis NASH Novartis Pharmaceuticals|Novartis October 24 2016 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

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