Leptomycin B

Synonyms: LMB, CI 940, Elactocin, Mantuamycin, NSC 364372

Leptomycin B (LMB, CI 940, Elactocin, Mantuamycin, NSC 364372) is a potent antifungal antibiotic isolated from Streptomyces and acts as a specific inhibitor of the nuclear export factor CRM1. Leptomycin B rapidly induces cytotoxic effects in cancer cell lines via covalent inhibition of CRM1 with IC50 values of 0.1 nM–10 nM.

Leptomycin B Chemical Structure

Leptomycin B Chemical Structure

CAS: 87081-35-4

Selleck's Leptomycin B has been cited by 2 publications

Purity & Quality Control

Batch: Purity: 99.74%
99.74

Leptomycin B Related Products

Choose Selective CRM1 Inhibitors

Biological Activity

Description Leptomycin B (LMB, CI 940, Elactocin, Mantuamycin, NSC 364372) is a potent antifungal antibiotic isolated from Streptomyces and acts as a specific inhibitor of the nuclear export factor CRM1. Leptomycin B rapidly induces cytotoxic effects in cancer cell lines via covalent inhibition of CRM1 with IC50 values of 0.1 nM–10 nM.
Targets
CRM1 [1]
In vitro
In vitro

Leptomycin B (LMB) is a potent anti-fungal antibiotic from <i>Streptomyces sp</i>., also can inhibit the expression of COX-2 mRNA and protein induced by serum withdrawal in MDA-MB-231 cells.<sup><a class="sref" href="#s_ref">[1]</a></sup><sup><a class="sref" href="#s_ref">[2]</a></sup>

Cell Research Cell lines MDA-MB-231 Cells
Concentrations 2.5, 5, 10, and 20 ng/ml
Incubation Time 24 h
Method

MDA-MB-231 cells were grown in culture medium containing 10% FBS without LMB (none or vehicle (v)) or in culture medium containing 0% FBS along with LMB in different concentrations (0, 2.5, 5, 10, and 20 ng/ml) for 24 h. Total RNA was isolated and analyzed for the COX-2 mRNA expression by Northern blot analysis, COX-2 protein expression by Western blot analysis.

In Vivo
In vivo

Leptomycin B Reduces Matrix Metalloproteinase-9 Expression and Suppresses Cutaneous Inflammation

Animal Research Animal Models Hairless mice
Dosages 20 mM, 2 mM, 200 nM, or 20 nM
Administration Topical
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03027388 Completed
Astrocytoma Grades II III and IV|Glioblastoma Multiforme|Giant Cell Glioblastoma|Glioma|Oligodendrogliomas
National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC)
January 9 2019 Phase 2
NCT03804879 Completed
Diabetic Nephropathy
Novartis Pharmaceuticals|Novartis
December 17 2018 Phase 2
NCT03436732 Terminated
Mesothelioma
National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC)
February 28 2018 Phase 1

Chemical Information & Solubility

Molecular Weight 540.73 Formula

C33H48O6

CAS No. 87081-35-4 SDF --
Smiles CCC(/C=C/C1OC(=O)C=CC1C)=C/C(C)C/C=C/C(C)=C/C(C)C(=O)C(C)C(O)C(C)C\C(C)=C\C(O)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (184.93 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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