Leptomycin B

Catalog No.S7580 Batch:S758001

Technical Data

Formula	C₃₃H₄₈O₆
Molecular Weight	540.73	CAS No.	87081-35-4
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (184.93 mM)
		Ethanol	100 mg/mL (184.93 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Leptomycin B (LMB, CI 940, Elactocin, Mantuamycin, NSC 364372) is a potent antifungal antibiotic isolated from Streptomyces and acts as a specific inhibitor of the nuclear export factor CRM1. Leptomycin B rapidly induces cytotoxic effects in cancer cell lines via covalent inhibition of CRM1 with IC50 values of 0.1 nM–10 nM.

Targets

CRM1 ^[1]

In vitro

Leptomycin B (LMB) is a potent anti-fungal antibiotic from Streptomyces sp., also can inhibit the expression of COX-2 mRNA and protein induced by serum withdrawal in MDA-MB-231 cells.<a class="sref" href="#s_ref">[1]</a><a class="sref" href="#s_ref">[2]</a>

In vivo

Leptomycin B Reduces Matrix Metalloproteinase-9 Expression and Suppresses Cutaneous Inflammation

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines MDA-MB-231 Cells Concentrations 2.5, 5, 10, and 20 ng/ml Incubation Time 24 h Method MDA-MB-231 cells were grown in culture medium containing 10% FBS without LMB (none or vehicle (v)) or in culture medium containing 0% FBS along with LMB in different concentrations (0, 2.5, 5, 10, and 20 ng/ml) for 24 h. Total RNA was isolated and analyzed for the COX-2 mRNA expression by Northern blot analysis, COX-2 protein expression by Western blot analysis.
Animal Study:^{^[2]}	Animal Models Hairless mice Dosages 20 mM, 2 mM, 200 nM, or 20 nM Administration Topical

Cell Assay:^{^[2]}

Cell lines
MDA-MB-231 Cells
Concentrations
2.5, 5, 10, and 20 ng/ml
Incubation Time
24 h
Method
MDA-MB-231 cells were grown in culture medium containing 10% FBS without LMB (none or vehicle (v)) or in culture medium containing 0% FBS along with LMB in different concentrations (0, 2.5, 5, 10, and 20 ng/ml) for 24 h. Total RNA was isolated and analyzed for the COX-2 mRNA expression by Northern blot analysis, COX-2 protein expression by Western blot analysis.

Animal Study:^{^[2]}

Animal Models
Hairless mice
Dosages
20 mM, 2 mM, 200 nM, or 20 nM
Administration
Topical

References

Selleck's Leptomycin B has been cited by 2 publications

N-acetyltransferase 10 promotes cutaneous wound repair via the NF-κB-IL-6 axis [ Cell Death Discov, 2023, 9(1):324]	PubMed: 37644005
Dickkopf1 fuels inflammatory cytokine responses [ Commun Biol, 2022, 5(1):1391]	PubMed: 36539532

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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