Home Others Others L-Citrulline For research use only.

L-Citrulline

L-citrulline is a substance called a non-essential amino acid that is used as a sports performance and cardiovascular health supplement.

L-Citrulline Chemical Structure

L-Citrulline Chemical Structure

CAS: 372-75-8

Purity & Quality Control

Batch: Purity: 99.89%
99.89

L-Citrulline Related Products

Biological Activity

Description L-citrulline is a substance called a non-essential amino acid that is used as a sports performance and cardiovascular health supplement.
In vitro
In vitro L-Citrulline restores nitric oxide/reactive oxygen species balance and barrier function in high glucose-treated monolayers of human glomerular endothelial cells. Moreover, L-Citrulline also has the ability to establish an anti-inflammatory profile, characterized by increased IL-10 and reduced IL-1β and IL-12(p70) generation in the human proximal tubular cells[1].
Cell Research Cell lines Human glomerular endothelial cells
Concentrations 1 mM
Incubation Time 2 h
Method Human glomerular endothelial cell are seeded at 1 × 105 cells per well in 24-well plates. Confluent quiescent cell monolayers are exposed to HG or proper iso-osmotic control for 24 h. L-Citrulline (1 mM) is applied 2 h prior to HG. Exposure is terminated by removal of the supernatant. Fresh basic CSC medium is replaced and cells incubated for additional 30 min. Supernatant is then removed, subsequently centrifuged and stored at −80°C for NO analysis.
In Vivo
In vivo Oral l-cit (precursor of l-arg) augments NO-dependent signaling, not only by means of increasing l-arg synthesis, but also by decreasing l-arg catabolism, as such increasing circulating l-arg levels. L-Citrulline exerts protective effects in kidneys of STZ-rats, and markedly reduces urinary albumin excretion, tubulo-interstitial fibrosis, and kidney hypertrophy, observed in untreated diabetic mice[1]. L-citrulline supplementation also strengthens vascular endothelium function and attenuates stress-induced cold hypersensitivity by improving blood circulation[2].
Animal Research Animal Models diabetic mice (C57BL/6 mice, intraperitoneal injections of STZ)
Dosages 50 mg/kg
Administration oral
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05636397 Recruiting
BPD - Bronchopulmonary Dysplasia|Pulmonary Hypertension|NEC
The Hospital for Sick Children
 November 1 2023 Not Applicable
NCT06092476 Not yet recruiting
Diabetes Mellitus Type 2
Academisch Medisch Centrum - Universiteit van Amsterdam (AMC-UvA)|Fractyl Health Inc.
 November 1 2023 Not Applicable
NCT06025383 Recruiting
Menopause|Hypertension
Texas Tech University
 July 20 2023 Not Applicable
NCT05590117 Enrolling by invitation
Colo-rectal Cancer|Neuropathy;Peripheral|Mucositis
Tanta University
 October 11 2022 Early Phase 1
NCT05253209 Recruiting
Ventricular Septal Defect|Atrioventricular Septal Defect|Primum Atrial Septal Defect
Asklepion Pharmaceuticals LLC
 June 29 2022 Phase 3

Chemical Information & Solubility

Molecular Weight 175.19 Formula

C6H13N3O3
CAS No. 372-75-8 SDF Download L-Citrulline SDF
Smiles C(CC(C(=O)O)N)CNC(=O)N
Storage (From the date of receipt)

In vitro
Batch:

Water : 35 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy L-Citrulline | L-Citrulline supplier | purchase L-Citrulline | L-Citrulline cost | L-Citrulline manufacturer | order L-Citrulline | L-Citrulline distributor