Home Immunology & Inflammation NOS chemical - Amino Acids and Derivatives chemical L-Arginine HCl (L-Arg)

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L-Arginine HCl (L-Arg) NOS chemical

Cat.No.S3174

L-Arginine HCl (L-Arg, (S)-(+)-Arginine hydrochloride) is the nitrogen donor for synthesis of nitric oxide, a potent vasodilator that is deficient during times of sickle cell crisis. This compound can be used to induce animal models of Acute Pancreatitis.
L-Arginine HCl (L-Arg) NOS chemical Chemical Structure

Chemical Structure

Molecular Weight: 210.66

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Chemical Information, Storage & Stability

Molecular Weight 210.66 Formula

C6H14N4O2.HCl

 Storage (From the date of receipt)
CAS No. 1119-34-2 Download SDF Storage of Stock Solutions

Synonyms (S)-(+)-Arginine hydrochloride Smiles C(CC(C(=O)O)N)CN=C(N)N.Cl

Solubility

In vitro
Batch:

Water : 42 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

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In vivo
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Mechanism of Action

In vitro

L-Arginine HCl (L-Arg) supplementation (0.3 mM, 30 minutes) does not induce any significant increases in the peak NO concentration at low level of native LDL. However, at native LDL concentrations from 60-130 mg cholesterol/dL, NO concentration is 2 times higher than before treatment in bovine aortic endothelial cells. This compound results in a significant increase of NO production in n-LDL–treated cells as well as in oxidized -LDL–treated cells in the same cell type. It does not increase O2- concentration at low nativeLDL concentrations but reduces O2- production by 50% when incubated with n-LDL at concentrations >40 mg cholesterol/dL. Furthermore, it completely abolishes O2- production at every oxidized LDL dosage in bovine aortic endothelial cells. [1]
In vivo

L-Arginine HCl (L-Arg) (4 mg/kg/min for 1 hour) treatment decreases superoxide generation by cNOS while increasing NO accumulation in rabbit limb during ischemia/reperfusion. This compound prevents microvessel constriction in the reperfused muscle despite reduced but still apparent interstitial edema in rabbit limb, and results in a significant reduction of muscular reperfusion edema in rabbit limb. [2] Its supplementation (0.1 g/kg, oral) significantly reduces pulmonary artery systolic pressure by a mean of 15.2% after 5 days of therapy in patients with sickle cell disease. Both L-Arginine and ornithine concentrations increased significantly after 5 days of oral supplementation in patients with sickle cell disease. [3] It is associated with a decrease in cardiac index while stroke index is maintained in patients with severe sepsis. Resolution of shock at 72 hours is achieved by 40% and 24% of the patients in the L-Arginine and placebo cohorts, respectively. [4] This compound (450 mg/kg during a 15-minute period) amplifies and sustains the hyperemia (38%) and increases absolute brain blood flow after eNOS upregulation by chronic simvastatin treatment (2 mg/kg subcutaneously, daily for 14 days) in SV-129 mice. [5]
References
  • [4] https://pubmed.ncbi.nlm.nih.gov/14707554/
  • [5] https://pubmed.ncbi.nlm.nih.gov/10779015/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06265623 Not yet recruiting
Chronic Obstructive Pulmonary Disease (COPD)
Universitätsklinikum Hamburg-Eppendorf
 March 1 2024 --
NCT06244758 Recruiting
Type2diabetes
University of Erlangen-Nürnberg Medical School|Bayer
 January 18 2024 Phase 3
NCT06137833 Not yet recruiting
Fatigue|Breast Cancer
Pharmanutra S.p.a.|Latis S.r.l.
 November 27 2023 Not Applicable
NCT05934318 Not yet recruiting
Pregnancy|Malaria|Nutrition|Placental Development|Preterm Birth|Fetal Growth Restriction
Liverpool School of Tropical Medicine|Kenya Medical Research Institute|University of Toronto|Telethon Kids Institute
 September 30 2023 Not Applicable

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