Home Microbiology Bacterial inhibitor GSK2200150A For research use only.

GSK2200150A

GSK2200150A is identified by high-throughput screening (HTS) campaign that can be used as an anti-tuberculosis (TB) agent (MIC = 0.38 µM).

GSK2200150A Chemical Structure

GSK2200150A Chemical Structure

CAS: 1443138-53-1

Purity & Quality Control

Batch: S042301 DMSO] 71 mg/mL] false] Ethanol] 18 mg/mL] false] Water] Insoluble] false Purity: 99.88%
99.88

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Biological Activity

Description GSK2200150A is identified by high-throughput screening (HTS) campaign that can be used as an anti-tuberculosis (TB) agent (MIC = 0.38 µM).
Targets
tuberculosis [1]
In vitro
In vitro

GSK2200150A is a novel antimycobacterial agent against Mycobacterium tuberculosis, by inhibiting the growth of virulent M.tuberculosis strain (H37Rv) with MIC of 0.38 μM.[1]
Cell Research Cell lines human monocytic cell line (THP1 cells)
Concentrations 1.56–200 µM
Incubation Time 4 days
Method

THP1 cells (1×105) in media containing 50 ng/mL phorbol 12-myristate 13-acetate (PMA) are added to 96-well plates and are then incubated for 48 h at 37°C to allow adherence and differentiation. GSK2200150A (1.56–200 µM) is added to the wells and incubated for 4 days at 37°C. Then 0.05%(w/v) resazurin (4 h) is added and the fluorescence measured. Cell viability is calculated as percentage fluorescence in comparison to untreated cells.

Chemical Information & Solubility

Molecular Weight 357.47 Formula

C20H23NO3S
CAS No. 1443138-53-1 SDF --
Smiles C1COC2(CCN(CC2)CC3=CC4=C(C=C3)OCCO4)C5=C1SC=C5
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 71 mg/mL ( (198.61 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 18 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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