GSK2200150A

Catalog No.S0423

For research use only.

GSK2200150A is identified by high-throughput screening (HTS) campaign that can be used as an anti-tuberculosis (TB) agent (MIC = 0.38 µM).

GSK2200150A Chemical Structure

CAS No. 1443138-53-1

Purity & Quality Control

Choose Selective Bacterial Inhibitors

Biological Activity

Description GSK2200150A is identified by high-throughput screening (HTS) campaign that can be used as an anti-tuberculosis (TB) agent (MIC = 0.38 µM).
Targets
tuberculosis [1]
In vitro

GSK2200150A is a novel antimycobacterial agent against Mycobacterium tuberculosis, by inhibiting the growth of virulent M.tuberculosis strain (H37Rv) with MIC of 0.38 μM.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: human monocytic cell line (THP1 cells)
  • Concentrations: 1.56–200 µM
  • Incubation Time: 4 days
  • Method:

    THP1 cells (1×105) in media containing 50 ng/mL phorbol 12-myristate 13-acetate (PMA) are added to 96-well plates and are then incubated for 48 h at 37°C to allow adherence and differentiation. GSK2200150A (1.56–200 µM) is added to the wells and incubated for 4 days at 37°C. Then 0.05%(w/v) resazurin (4 h) is added and the fluorescence measured. Cell viability is calculated as percentage fluorescence in comparison to untreated cells.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 357.47
Formula

C20H23NO3S

CAS No. 1443138-53-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1COC2(CCN(CC2)CC3=CC4=C(C=C3)OCCO4)C5=C1SC=C5

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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