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FICZ AhR agonist

Name: FICZ
Brand: Selleck Chemicals
SKU: S9682
Availability: InStock
Rating: 5 (5 reviews)

Cat.No.S9682

FICZ (6-Formylindolo[3,2-b]carbazole) is a potent agonist of aryl hydrocarbon receptor (AhR) that is efficiently metabolized by AHR-regulated cytochrome P4501 enzymes.

Chemical Structure

Molecular Weight: 284.31

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Quality Control

Batch: S968201 DMSO]57 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.05%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS
Datasheet

99.05

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other AhR Products	Stemregenin 1 (SR1) BAY 2416964 CH-223191 BAY-218 Tapinarof GNF351 L-Kynurenine YL-109 Diosmin PDM2

Solubility

In vitro
Batch:

DMSO : 57 mg/mL (200.48 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.500mg/ml (5.28mM)	Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	284.31	Formula	C₁₉H₁₂N₂O	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	172922-91-7	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	6-Formylindolo[3,2-b]carbazole			Smiles	O=CC1=C2C(=CC3=C1[NH]C4=C3C=CC=C4)[NH]C5=C2C=CC=C5

Mechanism of Action

Targets/IC₅₀/Ki	AhR
In vitro	In vitro studies in cells show that CYP1 inhibition in the presence of FICZ results in enhanced AHR activation, suggesting that this compound accumulates in the cell when its metabolism is blocked. It significantly upregulates the gene expression of filaggrin in both HaCaT cells and NHEKs in an AHR-dependent manner, but does not affect the gene expression of other barrier-related proteins.
In vivo	FICZ improves the atopic dermatitis-like skin inflammation, clinical scores, and transepidermal water loss in NC/Nga mice compared with those of control mice. On histology, this compound significantly reduces the epidermal and dermal thickness as well as the number of mast cells. Topical application also significantly reduces the gene expression of Il22.
References	[1] https://pubmed.ncbi.nlm.nih.gov/27112072/ [2] https://pubmed.ncbi.nlm.nih.gov/29500077/

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