FICZ

Catalog No.S9682 Batch:S968201

Technical Data

Formula

C₁₉H₁₂N₂O

Molecular Weight

284.31

CAS No.

172922-91-7

Solubility (25°C)*

In vitro

DMSO

57 mg/mL (200.48 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution

5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O

1.5mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

FICZ (6-Formylindolo[3,2-b]carbazole) is a potent agonist of aryl hydrocarbon receptor (AhR) that is efficiently metabolized by AHR-regulated cytochrome P4501 enzymes.

Targets

AhR ^[1]

In vitro

In vitro studies in cells show that CYP1 inhibition in the presence of FICZ results in enhanced AHR activation, suggesting that FICZ accumulates in the cell when its metabolism is blocked.^[1] FICZ significantly upregulates the gene expression of filaggrin in both HaCaT cells and NHEKs in an AHR-dependent manner, but does not affect the gene expression of other barrier-related proteins.^[2]

In vivo

FICZ improves the atopic dermatitis-like skin inflammation, clinical scores, and transepidermal water loss in NC/Nga mice compared with those of control mice. On histology, FICZ significantly reduces the epidermal and dermal thickness as well as the number of mast cells. Topical FICZ also significantly reduces the gene expression of Il22.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Human immortalized HaCaT cells, normal human epidermal keratinocytes (NHEKs) Concentrations 1 nM, 10 nM, 100 nM Incubation Time 1 h, 3 h, 5 h Method HaCaTcells or NHEKs (1×10⁴ cells/well) are platedon an 8-well μ-slide for 48 h, and treated with or without FICZ for 3 or 1 h, respectively. Then, the cells are washed with phosphate-buffered saline (PBS), fixed with acetone for 10 min, and blocked with 5% (w/v) bovine serum albumin in PBS for 30 min. The cells are then incubated with primary rabbit anti-AHR antibody (1:100) overnight at 4 ℃. Specific binding is detected using a horseradish peroxidase-conjugated goat anti-rabbit anti-body followed by tyramide labeling with green-fluorescent Alexa Fluor 488 for 1 h at room temperature in accordance with the manufacturer's instructions.
Animal Study:^{^[2]}	Animal Models 9–10 week-old female NC/Nga mice Dosages 100 mg/affected area Administration Topical

Cell Assay:^{^[2]}

Cell lines
Human immortalized HaCaT cells, normal human epidermal keratinocytes (NHEKs)
Concentrations
1 nM, 10 nM, 100 nM
Incubation Time
1 h, 3 h, 5 h
Method
HaCaTcells or NHEKs (1×10⁴ cells/well) are platedon an 8-well μ-slide for 48 h, and treated with or without FICZ for 3 or 1 h, respectively. Then, the cells are washed with phosphate-buffered saline (PBS), fixed with acetone for 10 min, and blocked with 5% (w/v) bovine serum albumin in PBS for 30 min. The cells are then incubated with primary rabbit anti-AHR antibody (1:100) overnight at 4 ℃. Specific binding is detected using a horseradish peroxidase-conjugated goat anti-rabbit anti-body followed by tyramide labeling with green-fluorescent Alexa Fluor 488 for 1 h at room temperature in accordance with the manufacturer's instructions.

Animal Study:^{^[2]}

Animal Models
9–10 week-old female NC/Nga mice
Dosages
100 mg/affected area
Administration
Topical

References

Selleck's FICZ has been cited by 3 publications

Tubular aryl hydratocarbon receptor upregulates EZH2 to promote cellular senescence in cisplatin-induced acute kidney injury [ Cell Death Dis, 2023, 14(1):18]	PubMed: 36635272
Intestinal Flora-Derived Kynurenic Acid Protects Against Intestinal Damage Caused by Candida albicans Infection via Activation of Aryl Hydrocarbon Receptor [ Front Microbiol, 2022, 13:934786]	PubMed: 35923391
Novel Pentapeptide Derived from Chicken by-Product Ameliorates DSS-Induced Colitis by Enhancing Intestinal Barrier Function via AhR-Induced Src Inactivation [ J Agric Food Chem, 2020, 10.1021/acs.jafc.0c06319]	PubMed: 33210912

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.