Euphorbiasteroid

Catalog No.S3832 Synonyms: Euphorbia factor L1

For research use only.

Euphorbiasteroid (Euphorbia factor L1), a component of Euphorbia lathyris L., inhibits adipogenesis of 3T3-L1 cells through activation of the AMPK pathway and induces HL-60 cells to apoptosis via promoting Bcl-2/Bax apoptotic signaling pathway in a dose-dependent manner. Euphorbiasteroid is a tricyclic diperpene of Euphorbia lathyris L., inhibits tyrosinase, and increases the phosphorylation of AMPK, with anti-cancer, anti-virus, anti-obesity and multidrug resistance-modulating effect.

Euphorbiasteroid Chemical Structure

CAS No. 28649-59-4

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Biological Activity

Description Euphorbiasteroid (Euphorbia factor L1), a component of Euphorbia lathyris L., inhibits adipogenesis of 3T3-L1 cells through activation of the AMPK pathway and induces HL-60 cells to apoptosis via promoting Bcl-2/Bax apoptotic signaling pathway in a dose-dependent manner. Euphorbiasteroid is a tricyclic diperpene of Euphorbia lathyris L., inhibits tyrosinase, and increases the phosphorylation of AMPK, with anti-cancer, anti-virus, anti-obesity and multidrug resistance-modulating effect.
In vitro

Euphorbiasteroid suppresses adipogenic differentiation of 3T3‐L1 cells, mainly at the early stage, and stimulates the AMPK signalling pathway. The anti-adipogenic effects of euphorbiasteroid could possibly be attributed to activation of the AMPK pathway, by decreasing the level of FAS and its up-regulators, including C/EBPs, PPAR-γ and SREBP-1c, without involving insulin signalling pathway[1]. Euphorbiasteroid could be a transport substrate for P-gp that can effectively inhibit P-gp-mediated drug transport and reverse resistance to anticancer drugs in MES-SA/Dx5 cells[2].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: The murine pre-adipocyte cell line 3T3‐L1
  • Concentrations: 0-50 μM
  • Incubation Time: 2 days
  • Method:

    3T3‐L1 cells were treated with euphorbiasteroid at concentrations of 6.25, 12.5, 25 and 50 µM for 2 days in adipogenesis induction medium, 2 days in adipogenesis medium and 2 days in culture medium (CM) sequentially during differentiation. Intracellular triglycerides (TGs) were stained with Oil red O (ORO) solution.

  • (Only for Reference)

Chemical Information

Molecular Weight 552.66
Formula

C32H40O8

CAS No. 28649-59-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1CC2(C(C1OC(=O)CC3=CC=CC=C3)C(C4(CCC5C(C5(C)C)C=C(C2=O)C)CO4)OC(=O)C)OC(=O)C

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