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CNO (Clozapine N-oxide)

Name: CNO (Clozapine N-oxide)
Brand: Selleck Chemicals
SKU: S6887
Rating: 5 (5 reviews)

Catalog No.S6887 Purity: 99.9%

CNO (Clozapine N-oxide) is a metabolite of Clozapine (GLXC-06516) and an agonist of human muscarinic designer receptors (Designer Receptors Exclusively Activated by Designer Drugs, DREADDs). Clozapine is a potent dopamine (DA) antagonist and a selective muscarinic M4 receptor agonist.Solutions are unstable and should be fresh-prepared.

CNO (Clozapine N-oxide) Chemical Structure

CAS: 34233-69-7

Selleck's CNO (Clozapine N-oxide) has been cited by 5 publications

Purity & Quality Control

Batch: Purity: 99.9%

99.9

Products often used together with CNO (Clozapine N-oxide)

JHU37152

Clozapine N-oxide exhibits lower in vivo DREADD potency, while JHU37152 exhibits high in vivo DREADD potency.

Bonaventura J, et al. Nat Commun. 2019 Oct 11;10(1):4627.

JHU37160

JHU37160 exhibits higher blood-brain barrier penetrance and DREADD selectivity in vivo than Clozapine N-oxide.

Savage JV, et al. Behav Brain Res. 2023 Aug 24;452:114553.

Deschloroclozapine

Clozapine N-oxide and Deschloroclozapine activate the DREADD to suppress neuronal activity in rhesus monkeys.

Eldridge MA, et al. Curr Res Neurobiol. 2023 Mar 22;4:100085.

DREADD agonist 21

Clozapine N-oxide and DREADD agonist 21 repeated administration contributes to an unusual organization of the gut microbiota in adult mice.

Guo W, et al. Brain Res Bull. 2021 Aug;173:66-73.

CNO (Clozapine N-oxide) Related Products

Related Targets	mAChR nAChR AChE	Click to Expand
Related Compound Libraries	FDA-approved Drug Library Natural Product Library Neuronal Signaling Compound Library CNS-Penetrant Compound Library Anti-alzheimer Disease Compound Library	Click to Expand

Signaling Pathway

AChR Signaling Pathway

Choose Selective AChR Inhibitors

Biological Activity

Description

Targets

DREADD ^[1]

Dopamine receptor ^[2]

muscarinic M4 receptor ^[1]

In vitro
In vitro	CNO is a synthetic small molecule actuator for muscarinic acetylcholine DREADDs.

In Vivo
In vivo	Clozapine N-oxide (CNO), an agonist of human muscarinic designer receptor (DREADDs), is converted to clozapine and exerts clozapine-like behavioral effects in both mice and rats further emphasizes the need for appropriate control groups in studies employing DREADDs.^[4]
Animal Research	Animal Models	standard laboratory rats and mice
	Dosages	1.0, 3.2, 10.0 mg/kg for rats; 1.25, 2.5, 5.0, 10.0, 20.0 mg/kg for mice
	Administration	i.p.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT00372242	Completed	Mild and Moderate Malnourished Children	International Centre for Diarrhoeal Disease Research Bangladesh	December 2000	Not Applicable

References

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Chemical Information & Solubility

Molecular Weight	342.82	Formula	C₁₈H₁₉ClN₄O
CAS No.	34233-69-7	SDF	--
Smiles	C[N+]1([O-])CCN(CC1)C2=NC3=C(NC4=CC=CC=C24)C=CC(=C3)Cl
Storage (From the date of receipt)	3 years -20°C powder	Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.

In vitro
Batch:

DMSO : 68 mg/mL ( (198.35 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 68 mg/mL

Ethanol : 8 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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