Palmatine

Catalog No.S3769 Synonyms: Berbericinine, Burasaine

For research use only.

Palmatine (Berbericinine, Burasaine), an isoquinoline alkaloid, has sedative, antidepressant, antioxidative, anti-ulcerative, antacid, anticancer, and anti-metastatic activities.

Palmatine Chemical Structure

CAS No. 3486-67-7

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Biological Activity

Description Palmatine (Berbericinine, Burasaine), an isoquinoline alkaloid, has sedative, antidepressant, antioxidative, anti-ulcerative, antacid, anticancer, and anti-metastatic activities.
Targets
AChE [3]
(Cell-free assay)
BChE [3]
(Cell-free assay)
0.51 μM 6.84 μM
In vitro

Palmatine plays an important role in osteoclast apoptosis via the NOS system in osteoclasts[1]. Palmatine has been reported to possess sedative and antioxidant activities. It has been also shown to be inhibitor of beta-site amyloid precursor protein-cleaving enzyme 1 (BACE 1), acetyl- and butyrylcholinesterases (AChE and BChE)[2].

In vivo Palmatine shows memory enhancing activity in mice probably by inhibiting brain acetylcholinesterase activity, through involvement of GABA-benzodiazepine pathway and due to its antioxidant activity[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: RAW 264.7 cells
  • Concentrations: 1, 5, 10, 40, and 100 µM
  • Incubation Time: 24 h or 5 days
  • Method: Cell viability is measured using the cell counting kit-8 (CCK-8). The cells are seeded in a 96-well flat-bottomed microplate (5 × 103 cells/well) and cultured in 100 μL of growth medium at 37°C and 5% CO2 for 24 h or 5 days. The cell culture medium in each well is then replaced with 100 μL of cell growth medium containing palmatine at the following concentrations: 1, 5, 10, 40, and 100 µM. Additionally, L-NAME (10, 100, and 1000 μM) dissolved in PBS was added. After incubation for 24 h or 5 days at 37°C, the cells are washed with PBS 3 times. Then, 10 μL of CCK-8 dye and 100 μL of α-MEM cell culture medium are added to each well, and cells are incubated for another 1 h at 37°C. Cell viability (%) is calculated.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: Swiss young male albino mice
  • Dosages: 0.1, 0.5, 1 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 70 mg/mL
(198.63 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 352.4
Formula

C21H22NO4

CAS No. 3486-67-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC(=C(C=C4CC3)OC)OC)OC

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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