Home Neuronal Signaling AChR inhibitor Palmatine For research use only.

Palmatine

Synonyms: Berbericinine, Burasaine

Palmatine (Berbericinine, Burasaine), an isoquinoline alkaloid, has sedative, antidepressant, antioxidative, anti-ulcerative, antacid, anticancer, and anti-metastatic activities.

Palmatine Chemical Structure

Palmatine Chemical Structure

CAS: 3486-67-7

Selleck's Palmatine has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 98.26%
98.26

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Signaling Pathway

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Biological Activity

Description Palmatine (Berbericinine, Burasaine), an isoquinoline alkaloid, has sedative, antidepressant, antioxidative, anti-ulcerative, antacid, anticancer, and anti-metastatic activities.
Targets
AChE [3]
(Cell-free assay)		 BChE [3]
(Cell-free assay)
0.51 μM 6.84 μM
In vitro
In vitro Palmatine plays an important role in osteoclast apoptosis via the NOS system in osteoclasts[1]. Palmatine has been reported to possess sedative and antioxidant activities. It has been also shown to be inhibitor of beta-site amyloid precursor protein-cleaving enzyme 1 (BACE 1), acetyl- and butyrylcholinesterases (AChE and BChE)[2].
Cell Research Cell lines RAW 264.7 cells
Concentrations 1, 5, 10, 40, and 100 µM
Incubation Time 24 h or 5 days
Method Cell viability is measured using the cell counting kit-8 (CCK-8). The cells are seeded in a 96-well flat-bottomed microplate (5 × 103 cells/well) and cultured in 100 μL of growth medium at 37°C and 5% CO2 for 24 h or 5 days. The cell culture medium in each well is then replaced with 100 μL of cell growth medium containing palmatine at the following concentrations: 1, 5, 10, 40, and 100 µM. Additionally, L-NAME (10, 100, and 1000 μM) dissolved in PBS was added. After incubation for 24 h or 5 days at 37°C, the cells are washed with PBS 3 times. Then, 10 μL of CCK-8 dye and 100 μL of α-MEM cell culture medium are added to each well, and cells are incubated for another 1 h at 37°C. Cell viability (%) is calculated.
In Vivo
In vivo Palmatine shows memory enhancing activity in mice probably by inhibiting brain acetylcholinesterase activity, through involvement of GABA-benzodiazepine pathway and due to its antioxidant activity[2].
Animal Research Animal Models Swiss young male albino mice
Dosages 0.1, 0.5, 1 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 352.4 Formula

C21H22NO4
CAS No. 3486-67-7 SDF Download Palmatine SDF
Smiles COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC(=C(C=C4CC3)OC)OC)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 70 mg/mL ( (198.63 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 20 mg/mL

Ethanol : 20 mg/mL


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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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