Home Microbiology Bacterial chemical Azelaic acid

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Azelaic acid Bacterial chemical

Azelaic acid (Nonanedioic acid, Finacea, Azelex, Anchoic acid) is an organic compound produced by the ozonolysis of oleic acid and possesses antibacterial, keratolytic, comedolytic, and anti-oxidant activity.

Azelaic acid Bacterial chemical Chemical Structure

Chemical Structure

Molecular Weight: 188.22

Purity & Quality Control

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Mechanism of Action

In vitro
In vitro AZ(Azelaic acid) combined with taurine (Tau) show more inhibitory effects in melanocytes than the treatment of this compound alone. This combination inhibits the melanin production and tyrosinase activity of B16F10 melanoma cells without significant cytotoxicity. Also inhibitory effects after treatment with these combined chemical are stronger than this acid alone on melanogenesis[2].
Cell Research Cell lines The mouse melanoma cell line B16F10
Concentrations 20 mM
Incubation Time 24 h
Method

B16F10 melanoma cells are seeded at a density of 2.5×105 cells/60 mm culture dish. The cells are treated with Azelaic acid combined with Tau for 24 hr. The cells pellets are dissolved in 1 N NaOH at 60°C for 1 hr. The relative melanin content is determined by measuring the absorbance at 475 nm in ELISA reader.

In Vivo
In vivo Azelaic acid exhibits an anti-atherogenic effect. Its athero-protective effect is not related to changes in plasma lipid content. This compound supplementation decreases the level of CD68 macrophage marker by 34% (p<0.05)[1].
Animal Research Animal Models Low density lipoprotein receptor knockout (LDLr−/−) mice
Dosages 0.50%
Administration oral administration

References
  • https://pubmed.ncbi.nlm.nih.gov/19880116/
  • https://pubmed.ncbi.nlm.nih.gov/20804622/

Chemical Information

Molecular Weight 188.22 Formula

C9H16O4
CAS No. 123-99-9 SDF Download SDF
Synonyms Nonanedioic acid, Finacea, Azelex, Anchoic acid
Smiles C(CCCC(=O)O)CCCC(=O)O

Storage and Stability

Storage (From the date of receipt)

In vitro
Batch:

DMSO : 37 mg/mL ( (196.57 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 37 mg/mL

Ethanol : 37 mg/mL


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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