Amikacin disulfate

Catalog No.S3065 Synonyms: BB-K8

For research use only.

Amikacin sulfate (BB-K8) binds to 16S rRNA (bacterial 30S ribosome), causing misreading of mRNA and supressing proteins synthesis.

Amikacin disulfate Chemical Structure

CAS No. 39831-55-5

Selleck's Amikacin disulfate has been cited by 1 Publication

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Biological Activity

Description Amikacin sulfate (BB-K8) binds to 16S rRNA (bacterial 30S ribosome), causing misreading of mRNA and supressing proteins synthesis.
Targets
16S rRNA [1]
In vitro

Amikacin sulfate has high affinities for 16S rRNA of the bacterial 30S ribosome, causing misreading of mRNA and leaving the bacterium unable to synthesize proteins vital to its growth. [1] Amikacin is active in vitro against Pseudomonas species, [2] Escherichia coli[3], Proteus species (indole-positive and indole-negative), Providencia species, Klebsiella-Enterobacter-Serratia species, Acinetobacter (formerly Mima-Herellea) species, and Citrobacter freundii.[4] [5] In vitro studies have shown that amikacin sulfate combined with a beta-lactam antibiotic acts synergistically against many clinically significant Gram-negative organisms. In vitro studies have shown that amikacin sulfate combined with beta-lactam antimicrobial agents including moxalactam or piperacillin acts synergistically against many clinically significant Gram-negative organisms. [6]

In vivo Amikacin (80 mg/kg i.v.) significantly reduced pseudomonal densities within aortic vegetations compared to controls in the experimental rabbit endocarditis model. In addition, amikacin given once daily is still more effective than the twice-daily regimen. [7] Amikacin given alone at a dose of 50 mg/kg, in one, two, or three divided doses, showed remarkable activity in model of Mycobacterium avium complex infections in beige mice. Addition of clofazimine increased the activity significantly. [8]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 781.76
Formula

C22H43N5O13.2H2SO4

CAS No. 39831-55-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C(C(C(C(C1NC(=O)C(CCN)O)OC2C(C(C(C(O2)CO)O)N)O)O)OC3C(C(C(C(O3)CN)O)O)O)N.OS(=O)(=O)O.OS(=O)(=O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04239326 Recruiting Diagnostic Test: targeted Next Generation Sequencing (tNGS) Tuberculosis Multidrug-Resistant Foundation for Innovative New Diagnostics Switzerland April 16 2021 --
NCT04677543 Recruiting Drug: ALIS|Drug: Azithromycin|Drug: Ethambutol|Drug: ELC Mycobacterium Infections Nontuberculous Insmed Incorporated December 22 2020 Phase 3
NCT04470973 Recruiting Drug: Cefuroxime|Drug: Amikacin Sepsis|Septic Shock|Infection Bacterial|Critically Ill Radboud University Medical Center July 15 2020 --

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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