Actinomycin D (Dactinomycin)

For research use only.

Catalog No.S8964 Synonyms: Act D, RASP-101

1 publication

Actinomycin D (Dactinomycin) Chemical Structure

Molecular Weight(MW): 1255.42

Actinomycin D (Dactinomycin) is a significant polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. Actinomycin D (Dactinomycin) inhibits DNA repair and rests the cell cycle at G1 phase with IC50 of 0.42 μM and 0.4 nM, respectively. Actinomycin D is an autophagy activator, induces p53-independent cell death and prolongs survival in high-risk chronic lymphocytic leukemia.

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Selleck's Actinomycin D (Dactinomycin) has been cited by 1 publication

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Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Actinomycin D (Dactinomycin) is a significant polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. Actinomycin D (Dactinomycin) inhibits DNA repair and rests the cell cycle at G1 phase with IC50 of 0.42 μM and 0.4 nM, respectively. Actinomycin D is an autophagy activator, induces p53-independent cell death and prolongs survival in high-risk chronic lymphocytic leukemia.
Targets
autophagy [3]
()
cell cycle [1]
(Cell-based assay)
DNA repair [2]
(Cell-free assay)
0.4 nM 0.42 μM
In vitro

Actinomycin D markedly reduces the SMC proliferation via the inhibition of BrdU incorporation at 80 nM. This is further supported by the G1-phase arrest using a flowcytometric analysis. The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf are all suppressed by actinomycin D. Extracellular signalregulated kinases (Erk) involved in cell-cycle arrest are found to be increased by actinomycin D.[1]

In vivo

Actinomycin D targets survival proteins TOSO, BCL2 and MCL1 and is active in two different mouse models that are characterized by either unmutated B-cell receptor or inactive p53 function, both of which are known negative prognostic factors in CLL.[3]

Protocol

Cell Research:

[1]

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  • Cell lines: A10 cells (Vascular SMC)
  • Concentrations: 80 nM, 800 nM, 8 μM
  • Incubation Time: 18-24 h
  • Method:

    Cultured SMC are starved for 24 h followed by incubation with various doses of actinomycin D at 37℃. Drug treatment is carried out for 18-24 h. Since actinomycin D is dissolved in 0.1% DMSO, a vehicle control containing DMSO is also included.


    (Only for Reference)
Animal Research:

[3]

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  • Animal Models: C57BL/6 wild-type mice engrafted with tumor cells from Em-TCL-1 transgenic mice
  • Dosages: 0.06 mg/kg
  • Administration: IP
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (79.65 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 1255.42
Formula

C62H86N12O16

CAS No. 50-76-0
Storage powder
in solvent
Synonyms Act D, RASP-101

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04323189 Suspended Drug: Sitagliptin 100mg|Drug: Placebo Oral Tablet Genetics Disease|Type2 Diabetes|Heart Failure University of Pennsylvania March 1 2020 Phase 4
NCT04158648 Active not recruiting Drug: Emicizumab Mild Hereditary Factor VIII Deficiency Disease Without Inhibitor|Moderate Hereditary Factor VIII Deficiency Disease Without Inhibitor|Hemophilia A Hoffmann-La Roche February 10 2020 Phase 3
NCT03078907 Completed Drug: Selexipag|Drug: Placebo Pulmonary Arterial Hypertension Actelion November 8 2017 Phase 4
NCT04200807 Recruiting Device: Ultrasound Cardiac Output Monitor (USCOM 1A) Pre-Term|Neonatal Infection Vilnius University September 12 2017 --
NCT04032600 Recruiting Procedure: Paracentesis Malignant Ascites|Ovarian Cancer|Hemodynamic Instability Charite University Berlin Germany August 1 2017 Not Applicable

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID