Actinomycin D (Dactinomycin)
For research use only.
Catalog No.S8964 Synonyms: Act D, RASP-101
Molecular Weight(MW): 1255.42
Actinomycin D (Dactinomycin) is a significant polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. Actinomycin D (Dactinomycin) inhibits DNA repair and rests the cell cycle at G1 phase with IC50 of 0.42 μM and 0.4 nM, respectively. Actinomycin D is an autophagy activator, induces p53-independent cell death and prolongs survival in high-risk chronic lymphocytic leukemia.
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|Description||Actinomycin D (Dactinomycin) is a significant polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. Actinomycin D (Dactinomycin) inhibits DNA repair and rests the cell cycle at G1 phase with IC50 of 0.42 μM and 0.4 nM, respectively. Actinomycin D is an autophagy activator, induces p53-independent cell death and prolongs survival in high-risk chronic lymphocytic leukemia.|
Actinomycin D markedly reduces the SMC proliferation via the inhibition of BrdU incorporation at 80 nM. This is further supported by the G1-phase arrest using a flowcytometric analysis. The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf are all suppressed by actinomycin D. Extracellular signalregulated kinases (Erk) involved in cell-cycle arrest are found to be increased by actinomycin D.
Actinomycin D targets survival proteins TOSO, BCL2 and MCL1 and is active in two different mouse models that are characterized by either unmutated B-cell receptor or inactive p53 function, both of which are known negative prognostic factors in CLL.
|In vitro||DMSO||100 mg/mL (79.65 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
|Synonyms||Act D, RASP-101|
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04323189||Suspended||Drug: Sitagliptin 100mg|Drug: Placebo Oral Tablet||Genetics Disease|Type2 Diabetes|Heart Failure||University of Pennsylvania||March 1 2020||Phase 4|
|NCT04158648||Active not recruiting||Drug: Emicizumab||Mild Hereditary Factor VIII Deficiency Disease Without Inhibitor|Moderate Hereditary Factor VIII Deficiency Disease Without Inhibitor|Hemophilia A||Hoffmann-La Roche||February 10 2020||Phase 3|
|NCT03078907||Completed||Drug: Selexipag|Drug: Placebo||Pulmonary Arterial Hypertension||Actelion||November 8 2017||Phase 4|
|NCT04200807||Recruiting||Device: Ultrasound Cardiac Output Monitor (USCOM 1A)||Pre-Term|Neonatal Infection||Vilnius University||September 12 2017||--|
|NCT04032600||Recruiting||Procedure: Paracentesis||Malignant Ascites|Ovarian Cancer|Hemodynamic Instability||Charite University Berlin Germany||August 1 2017||Not Applicable|
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