For research use only.
Catalog No.S2358 Synonyms: Silybin B
CAS No. 65666-07-1
Silymarin (Silybin B), a polyphenolic flavonoid extracted from the seeds of Silybum marianum or milk thistle, is used in the prevention and treatment of liver diseases and primary liver cancer.
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Silymarin improved HFD-induced hepatic injury. Mice were fed with NCD or HFD for 14 weeks, then treated with silymarin for 3 weeks, followed by 12 h fasting. (a) ALT and AST levels in serum were determined. (b) Hematoxylin and eosin staining of liver (400×) was showed. Values are means ± SD (n = 8). *p < 0.05 and **p < 0.01 compared to HFD group.
Pharm Biol, 2016, 8:1-6.. Silymarin purchased from Selleck.
Purity & Quality Control
|Description||Silymarin (Silybin B), a polyphenolic flavonoid extracted from the seeds of Silybum marianum or milk thistle, is used in the prevention and treatment of liver diseases and primary liver cancer.|
Silymarin (Silybin B), a polyphenolic flavonoid extracted from the seeds of Silybum marianum or milk thistle, is used in the prevention and treatment of liver diseases and primary liver cancer.  The mechanisms of action of silymarin involve different biochemical events, such as the stimulation of the synthetic rate of ribosomal RNA (rRNA) species through stimulation of polymerase I and rRNA transcription, protecting the cell membrane from radical-induced damage and blockage of the uptake of toxins such as alpha-amanitin.  Silymarin can also provide substantial protection against different stages of UVB-induced carcinogenesis, possibly via its strong antioxidant properties. sup>
|In vivo||LD50: Mice 391.4mg/kg (i.v.); Rats 370.1mg/kg (i.v.); Rabbits 142.3mg/kg (i.v.) sup>|
|In vitro||DMSO||96 mg/mL warmed (198.98 mM)|
|Ethanol||1 mg/mL (2.07 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation ()|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04434404||Completed||Drug: L-Carnitine 500Mg Oral Tablet||Breast Cancer||Horus University||September 10 2018||Phase 4|
|NCT03216668||Unknown status||Drug: TONKA|Drug: LEGALON||Liver Function Failure||Nhat Nhat Pharmaceutical Company||June 15 2017||Phase 3|
|NCT02006498||Completed||Drug: Sillymarin|Drug: Placebo||Non-alcoholic Fatty Liver Disease||University of Malaya|Rottapharm||June 2012||Phase 2|
|NCT01018615||Completed||Drug: silymarin|Dietary Supplement: green tea extract (EGCG)||Chronic Hepatitis C|Oxidative Stress||University of North Carolina Chapel Hill|National Center for Complementary and Integrative Health (NCCIH)|National Institutes of Health (NIH)||November 2009||Phase 1|
|NCT00055718||Completed||Dietary Supplement: silymarin||Drug/Agent Toxicity by Tissue/Organ|Leukemia||Herbert Irving Comprehensive Cancer Center|National Cancer Institute (NCI)||November 2001||Phase 2|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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