JNJ-26854165 (Serdemetan)

Name: JNJ-26854165 (Serdemetan)
Brand: Selleck Chemicals
SKU: S1172
Rating: 5 (11 reviews)

For research use only.

Catalog No.S1172

11 publications

JNJ-26854165 (Serdemetan) Chemical Structure

CAS No. 881202-45-5

JNJ-26854165 (Serdemetan) acts as a HDM2 ubiquitin ligase antagonist and also induces early apoptosis in p53 wild-type cells, inhibits cellular proliferation followed by delayed apoptosis in the absence of functional p53. Phase 1.

Selleck's JNJ-26854165 (Serdemetan) has been cited by 11 publications

Cell, 2019, 36(2):179-193

PubMed: 28162770 ( click the link to review the publication )

Cancer Cell, 2019, 36(2):179-193

PubMed: 31378681 ( click the link to review the publication )

Cancer Res, 2020, canres.3499.2019

PubMed: 33372039 ( click the link to review the publication )

Circulation, 2020, 10.1161/CIRCULATIONAHA.120.048191

PubMed: 32755395 ( click the link to review the publication )

BMC Pharmacol Toxicol, 2017, 18(1):40

PubMed: 28577368 ( click the link to review the publication )

Oncotarget, 2017, 8(5):7777-7790

PubMed: 27999193 ( click the link to review the publication )

Sci Signal, 2016, 9(448):ra98

PubMed: 27703031 ( click the link to review the publication )

Mol Ther, 2015, 23(5):857-65

PubMed: 25648263 ( click the link to review the publication )

Sci Rep, 2014, 4:4664

PubMed: 24722541 ( click the link to review the publication )

Head Neck, 2014, 10.1002/hed.23822

PubMed: 24986420 ( click the link to review the publication )

Assay Drug Dev Technol, 2014, 12(9-10):514-26

PubMed: 25506801 ( click the link to review the publication )

6 Customer Reviews

Mol Pharmacol 2014 85(3), 408-19. JNJ-26854165 (Serdemetan) purchased from Selleck.

Sci Rep 2014 4, 4663. JNJ-26854165 (Serdemetan) purchased from Selleck.
Sci Rep 2014 4, 4663. JNJ-26854165 (Serdemetan) purchased from Selleck.

Sci Rep 2014 4, 4663. JNJ-26854165 (Serdemetan) purchased from Selleck.
J Nat Med 2013 10.1007/s11418-014-0825-0. JNJ-26854165 (Serdemetan) purchased from Selleck.

Curr Protoc Chem Biol 2013 5(3), 195-212. JNJ-26854165 (Serdemetan) purchased from Selleck.

Purity & Quality Control

Choose Selective p53 Inhibitors

Biological Activity

Description

Targets

p53 ^[1]
(Cell-free assay)

HDM2 ^[1]
(Cell-free assay)

Mdm2 ^[2]
(Cell-free assay)

In vitro

JNJ 26854165 is a novel tryptamine derivative which activates p53 and acts as a HDM2 ubiquitin ligase antagonist. JNJ 26854165 inhibits cell growth and induces apoptosis in leukemia cell lines with IC50 values of 0.24, 0.33, 0.32 and 0.44 μM at 72 hours for OCI-AML-3, MOLM-13, NALM-6 and REH cells, respectively. In addition, JNJ 26854165 accelerates proteasome-mediated degradation of p21 and antagonizes the transcriptional induction of p21 by p53. It also induces S-phase delay and upregulates E2F1 expression in p53 mutant cells, resulting in preferential apoptosis of S-phase cells. ^[1] JNJ 26854165 is an oral Mdm2 inhibitor which can inhibit the interaction of Mdm2-p53 complex with the proteasome and increase p53 levels by binding to RING domain of Mdm2. ^[2] A recent study shows that JNJ 26854165 inhibits clonogenic survival in four human cancer cell lines: H460, A549, p53-WT-HCT116, and p53-null-HCT116. ^[3]

Cell Data

Cell Lines	Assay Type	Activity Description	PMID
KU-19-19 cell	M{\lb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NGXKfJFKdmirYnn0bY9vKG:oIHj1cYFvKEuXLUG5MVE6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC57M{KzOkDPxE1?	NEXYfoRUSU6JRWK=
H4 cell	NIXVU5dIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	MkX0TY5pcWKrdHnvckBw\iCqdX3hckBJPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTFwME[3NFch\|ryP	M4XXO3NCVkeHUh?=
KGN cell	MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NEnPdGlKdmirYnn0bY9vKG:oIHj1cYFvKEuJTjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUGuOlIxPSEQvF2=	NWHrSWxDW0GQR1XS
786-0 cell	NX7pdlZGT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	MVHJcohq[mm2aX;uJI9nKGi3bXHuJFc5Pi1yIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;MT63Nlk5QSEQvF2=	NHOyRmRUSU6JRWK=
769-P cell	NIjpUIhIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NFXr[4tKdmirYnn0bY9vKG:oIHj1cYFvKDd4OT3QJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU45OjJ{NDFOwG0>	M{XoVHNCVkeHUh?=
K5 cell	NHzSTpNIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NHTvNGJKdmirYnn0bY9vKG:oIHj1cYFvKEt3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;Mj6wNVYzPCEQvF2=	NH3JXHJUSU6JRWK=
MLMA cell	M{PpWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NUXNcmVlUW6qaXLpeIlwdiCxZjDoeY1idiCNT2PDMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjB\|MkG3JO69VQ>?	NGW5ZpJUSU6JRWK=
MLMA cell	MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	MXHJcohq[mm2aX;uJI9nKGi3bXHuJG1NVUFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{LkK4O\|A{KM7:TR?=	MVfTRW5ITVJ?
EW-7 cell	MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	MmnOTY5pcWKrdHnvckBw\iCqdX3hckBGXy15IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;Mj6zNlY6PSEQvF2=	NF3EWpNUSU6JRWK=
SW1088 cell	NEjVeJRIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NYHWfFJqUW6qaXLpeIlwdiCxZjDoeY1idiCVV{GwPFgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjhyNkWxJO69VQ>?	NWfhdmZHW0GQR1XS
MC-IXC cell	MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NXrnfVR6UW6qaXLpeIlwdiCxZjDoeY1idiCPQz3JXGMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjh5N{myJO69VQ>?	NEfU[5hUSU6JRWK=
NCI-H2052 cell	MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	M3rM[GlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFUzKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5yOEG1O{DPxE1?	M1TkXHNCVkeHUh?=
IGR-1 cell	MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NV3qVldHUW6qaXLpeIlwdiCxZjDoeY1idiCLR2KtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvOTJyMkig{txO	MVzTRW5ITVJ?
DSH1 cell	NUPlU\|dJT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	Ml[1TY5pcWKrdHnvckBw\iCqdX3hckBFW0hzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;Mz6yOFI6PiEQvF2=	NILzXmVUSU6JRWK=
PA-1 cell	MoezS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	MkDaTY5pcWKrdHnvckBw\iCqdX3hckBRSS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;Mz6zNFA2Qc7:TR?=	MWrTRW5ITVJ?
SK-MEL-3 cell	M3vrO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7	MkTaTY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvOzF{MEeg{txO	NH;KXppUSU6JRWK=
SW900 cell	NFP1cHhIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	Moe1TY5pcWKrdHnvckBw\iCqdX3hckBUXzlyMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUOuOFgzPTVizszN	MkXKV2FPT0WU
CAKI-1 cell	MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	Mki4TY5pcWKrdHnvckBw\iCqdX3hckBESUuLLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlUzPzN6IN88US=>	MmTrV2FPT0WU
ES1 cell	NH7PelZIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NHP5d\|FKdmirYnn0bY9vKG:oIHj1cYFvKEWVMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUOuOVI5QTdizszN	NWrJW5BLW0GQR1XS
SK-N-DZ cell	MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NFLTSnBKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSHoh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjZ{NUm1JO69VQ>?	NY\UVmZVW0GQR1XS
RH-1 cell	M1njVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	MoHKTY5pcWKrdHnvckBw\iCqdX3hckBTUC1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;Mz62PFQ3OyEQvF2=	MVnTRW5ITVJ?
ES8 cell	MnXjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	NXLLc3hrUW6qaXLpeIlwdiCxZjDoeY1idiCHU{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlY6PzB{IN88US=>	NYLrfXBYW0GQR1XS
NEC8 cell	NFG2NYdIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	MYTJcohq[mm2aX;uJI9nKGi3bXHuJG5GSzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1\|Lke2PVgzKM7:TR?=	NHPte21USU6JRWK=
LNCaP-Clone-FGC cell	NVPJNFIzT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	M{XJb2lvcGmkaYTpc44hd2ZiaIXtZY4hVE6FYWCtR4xwdmVvRlfDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{45OzV\|NjFOwG0>	NUfkOJdbW0GQR1XS
HCE-4 cell	NX;6cmdTT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	MVnJcohq[mm2aX;uJI9nKGi3bXHuJGhETS12IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;Mz65PFA4KM7:TR?=	MlHOV2FPT0WU
U-118-MG cell	NITFfHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	MmfuTY5pcWKrdHnvckBw\iCqdX3hckBWNTFzOD3NS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvODFyNk[g{txO	NVuxcGQzW0GQR1XS
GI-ME-N cell	NXn5e4tkT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	NXfNSYtkUW6qaXLpeIlwdiCxZjDoeY1idiCJST3NSU1PKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC5yOEe5OkDPxE1?	M1zBfHNCVkeHUh?=
LB1047-RCC cell	MnX3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	MlnETY5pcWKrdHnvckBw\iCqdX3hckBNSjFyNEetVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC5zNkG0N{DPxE1?	NULUZWNrW0GQR1XS
HT-1080 cell	NEe5d41Iem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NY\LRVhLUW6qaXLpeIlwdiCxZjDoeY1idiCKVD2xNFgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC5zOUmyNUDPxE1?	MUjTRW5ITVJ?
NB69 cell	NIjZdVhIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	M{XndWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJ4OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUSuN\|UxPDdizszN	MlnKV2FPT0WU
NCI-H1693 cell	NFO5TpZIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NGjmTHZKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVY6OyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTRwNES4PVUh\|ryP	M3\ENHNCVkeHUh?=
HSC-3 cell	MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	MmTQTY5pcWKrdHnvckBw\iCqdX3hckBJW0NvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUSuOFk6OzNizszN	M3jkWnNCVkeHUh?=
MDA-MB-231 cell	M1X1PGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NH\kWYpKdmirYnn0bY9vKG:oIHj1cYFvKE2GQT3NRk0zOzFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12LkW1PFk6KM7:TR?=	M1LjfHNCVkeHUh?=
HOS cell	MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	MWjJcohq[mm2aX;uJI9nKGi3bXHuJGhQWyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTRwN{i2N{DPxE1?	Ml7OV2FPT0WU
BT-549 cell	M4nLemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NVvWclQzUW6qaXLpeIlwdiCxZjDoeY1idiCEVD21OFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01Njd6OEeg{txO	NYnmRmhHW0GQR1XS
NB17 cell	M{O2Nmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NXjj[JF1UW6qaXLpeIlwdiCxZjDoeY1idiCQQkG3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE44QDl5NjFOwG0>	MmnrV2FPT0WU
5637 cell	MoHlS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	M2D3emlvcGmkaYTpc44hd2ZiaIXtZY4hPTZ\|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUSuO\|k3PTJizszN	MoHyV2FPT0WU
OVCAR-8 cell	MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	MXvJcohq[mm2aX;uJI9nKGi3bXHuJG9XS0GULUigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlkzPDN4IN88US=>	MorrV2FPT0WU
G-402 cell	NVrRSoxrT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGcuPDB{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;ND65N\|A{PSEQvF2=	NVW3bYp1W0GQR1XS
BB30-HNC cell	NEDoS5JIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	MXvJcohq[mm2aX;uJI9nKGi3bXHuJGJDOzBvSF7DJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4xPzJzODFOwG0>	MV;TRW5ITVJ?
HCC1806 cell	MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	MXPJcohq[mm2aX;uJI9nKGi3bXHuJGhESzF6ME[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlA5OzN7IN88US=>	M1HKeXNCVkeHUh?=
COLO-800 cell	MoqzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	M4j4fmlvcGmkaYTpc44hd2ZiaIXtZY4hS0:OTz24NFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjF{NkSg{txO	MVvTRW5ITVJ?
FADU cell	MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NX7mVllIUW6qaXLpeIlwdiCxZjDoeY1idiCIQVTVJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4{PjV3NzFOwG0>	NGDjTpVUSU6JRWK=
NCI-H1651 cell	M4DycGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	MlzUTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG2OVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjN7NEC5JO69VQ>?	MnPsV2FPT0WU
AGS cell	NGexflNIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NHXhR3NKdmirYnn0bY9vKG:oIHj1cYFvKEGJUzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUWuOFc4OTFizszN	NHfGUmZUSU6JRWK=
CHP-212 cell	NV7MSldET3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	M2TOSWlvcGmkaYTpc44hd2ZiaIXtZY4hS0iSLUKxNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvOzl2MEmg{txO	NXPFNZpwW0GQR1XS
YAPC cell	MnKxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	NIGxU49KdmirYnn0bY9vKG:oIHj1cYFvKFmDUFOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlQ4PzFzIN88US=>	NVrPc2RWW0GQR1XS
GOTO cell	MoqyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	M4npSWlvcGmkaYTpc44hd2ZiaIXtZY4hT0:WTzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUWuOFk2PzZizszN	NI[2NHdUSU6JRWK=
KYSE-510 cell	MmSxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	MYnJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvNUGwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU42OTFizszN	MWLTRW5ITVJ?
NCI-H2342 cell	M2P1dWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	M3zTZmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyN\|QzKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS53M{K3PEDPxE1?	M2jJUHNCVkeHUh?=
BFTC-905 cell	M4Lv[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7	MYHJcohq[mm2aX;uJI9nKGi3bXHuJGJHXENvOUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU43Ojh\|NTFOwG0>	NVjYcpBMW0GQR1XS
EW-16 cell	NYftT4d7T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	Mn\UTY5pcWKrdHnvckBw\iCqdX3hckBGXy1zNjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUWuO\|IxQTNizszN	MUfTRW5ITVJ?
SK-MEL-30 cell	NFWyWWJIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	Mn\wTY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytN\|Ah[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njd2NUSzJO69VQ>?	NF7PZpNUSU6JRWK=
HLE cell	NWqzc5BjT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	MlnUTY5pcWKrdHnvckBw\iCqdX3hckBJVEViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lke3OFg6KM7:TR?=	NX7MZYx6W0GQR1XS
T98G cell	NG[zVodIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	MoHtTY5pcWKrdHnvckBw\iCqdX3hckBVQTiJIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;NT63PVY{PSEQvF2=	MnrlV2FPT0WU
HUTU-80 cell	M2i2OGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NW\he4hvUW6qaXLpeIlwdiCxZjDoeY1idiCKVWTVMVgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS56NEKyPEDPxE1?	MmDuV2FPT0WU
NOS-1 cell	NX\k[olzT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	NITWWm9KdmirYnn0bY9vKG:oIHj1cYFvKE6RUz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46PDd4NjFOwG0>	NIPT[GtUSU6JRWK=
SW780 cell	MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NEXwc45KdmirYnn0bY9vKG:oIHj1cYFvKFOZN{iwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46Pjl2OTFOwG0>	M4PxUXNCVkeHUh?=
KYSE-180 cell	MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NGnwb25KdmirYnn0bY9vKG:oIHj1cYFvKEu\U1WtNVgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57N{[1OEDPxE1?	NILJTYpUSU6JRWK=
MDA-MB-361 cell	MknQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	NUDLXWkxUW6qaXLpeIlwdiCxZjDoeY1idiCPRFGtUWIuOzZzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;NT65PFY1PyEQvF2=	MWXTRW5ITVJ?
SNU-C2B cell	MmjlS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	M1TtV2lvcGmkaYTpc44hd2ZiaIXtZY4hW06XLVOyRkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvODFzM{ig{txO	MX7TRW5ITVJ?
NCI-H661 cell	NXzZWndxT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	NIjW[5BKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOlYyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi5yNkm0JO69VQ>?	MUfTRW5ITVJ?
OE33 cell	NWD4W4VyT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	M{PyfmlvcGmkaYTpc44hd2ZiaIXtZY4hV0V\|MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPU[uNVQ{QDlizszN	MV3TRW5ITVJ?
TYK-nu cell	M3m3PGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NEnZNFhKdmirYnn0bY9vKG:oIHj1cYFvKFS\Sz3ueUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvOjF6NkOg{txO	MVTTRW5ITVJ?
COLO-792 cell	MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	MV;Jcohq[mm2aX;uJI9nKGi3bXHuJGNQVE9vN{myJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4zPTJzOTFOwG0>	MV;TRW5ITVJ?
HEL cell	MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	Mn[yTY5pcWKrdHnvckBw\iCqdX3hckBJTUxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14LkK2O\|Yh\|ryP	M3rBfnNCVkeHUh?=
D-566MG cell	MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	M{f2eWlvcGmkaYTpc44hd2ZiaIXtZY4hTC13Nk\NS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvOzN2OUGg{txO	NGfsWI5USU6JRWK=
U031 cell	MojnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	MVnJcohq[mm2aX;uJI9nKGi3bXHuJHUxOzFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14LkSxPFE3KM7:TR?=	NIWzZYRUSU6JRWK=
COR-L23 cell	M1G0TWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	M33mR2lvcGmkaYTpc44hd2ZiaIXtZY4hS0:ULVyyN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvPDN\|NE[g{txO	NGXVW2ZUSU6JRWK=
NCI-H2452 cell	MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NFnIcYNKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlQ2OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTZwNUKyPFEh\|ryP	NG[1fYFUSU6JRWK=
BB65-RCC cell	NGPCcm1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?=	MXzJcohq[mm2aX;uJI9nKGi3bXHuJGJDPjVvUlPDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok43PDV7IN88US=>	NXLqSYV6W0GQR1XS
CAL-33 cell	NWPhSVN3T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	M1jiSmlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUOzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok43PTZ4NTFOwG0>	M1jWZXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p53 / HDM2 / HDMX / p21 / Noxa / Puma ; PubMed: 20736344 Mol Cancer Ther OCI-AML-3 and MOLM-13 cells were incubated with a range of concentrations of JNJ-26854165 for 18 hours. ABCA1 / ABCG1 / NPC1 / NPC2 / HMGcoR ; PubMed: 23820125 J Pharmacol Exp Ther U266 and JeKo-1 cells were treated with JNJ-26854165 and cells harvested at the indicated time points. MDM2 / p-MDM2 / p53 ; PubMed: 27999193 Oncotarget K562 cells was harvested at 48 h after treatment with various JNJ-165 concentrations, and then nuclear and cytoplasmic extracts were prepared to check the levels of p53, MDM2 and p-MDM2 by Western blotting. Actin and LaminB were used as loading control respectively. Bcr-abl / p-Bcr-abl / p-CrkL / STAT5 / p-STAT5 / STAT3 / p-STAT3 ; PubMed: 27999193 Oncotarget CML cell lines were treated with JNJ-165 at the indicated doses for 48 h. Whole-cell lysates were extracted to assess the levels of BCR-ABL and phosphorylated (p)-BCR-ABL (Tyr177), and then were analyzed for BCR-ABL downstream signaling mediators p-CrkL (Try207), total Stat5, p-Stat5 (Tyr694), total Stat3, and p-Stat3 (Ser727) expression by Western blot analysis. The expression of β-actin was used as loading control. The data are representative of three determinations with identical results.	20736344 23820125 27999193
Growth inhibition assay	Cell viability; PubMed: 23820125 J Pharmacol Exp Ther MCL (B) and MM (C) cell lines were seeded in 96-well plates for viability analyses using WST-1 and treated with JNJ-26854165 for 72 hours. Results are expressed as the percentage of cell viability in relation to the vehicle-treated sample for each cell line, which was arbitrarily set at 100%	23820125
Immunofluorescence	p53; PubMed: 27999193 Oncotarget Two-color fluorescence microscope analysis of antibody against p53 protein (green) and nuclei (DAPI staining, blue) showed nuclear accumulation of p53 in K562 cells with treatment of 1 μM JNJ-165, but not in control-treated cells after 48 h. Images were taken on a fluorescence microscopy (100×).	27999193

In vivo

JNJ 26854165 leads to significant differences in EFS distribution in 17 of the 36 (47%) evaluable solid tumor xenografts and in 5 of 7 (71%) of the evaluable ALL xenografts using a dose of 20 mg/kg administered via oral gavage daily for 5 days, repeated for 6 weeks. ^[4]

Protocol

Cell Research:^[1]

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Cell lines: OCI-AML-3, MOLM-13, NB4 and U937 cells
Concentrations: 0-10 μM
Incubation Time: 72 hours
Method: Cell lines are maintained in RPMI 1640 medium containing 10% heat-inactivated fetal calf serum (FCS). OCI-AML-3, MOLM-13, NB4 and U937 cells are derived from acute myelogenous leukemia (AML) patients, K562 from a chronic myelogenous leukemia (CML) patient in blast crisis, and NALM-6, REH, P12-ICHIK
(Only for Reference)

Animal Research:^[4]

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Animal Models: CB17SC scid^-/- female mice.
Dosages: ≤20 mg/kg
Administration: Administered via p.o.
(Only for Reference)

References

[4] Smith MA, et al. Pediatr Blood Cancer, 2012, 59(2), 329-332.

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Solubility (25°C)

In vitro	DMSO	66 mg/mL (200.96 mM)
	Ethanol	2 mg/mL (6.08 mM)
	Water	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 1% DMSO+30% polyethylene glycol+1% Tween 80 For best results, use promptly after mixing.	30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download JNJ-26854165 (Serdemetan) SDF

Molecular Weight	328.41
Formula	C₂₁H₂₀N₄
CAS No.	881202-45-5
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	C1=CC=C2C(=C1)C(=CN2)CCNC3=CC=C(C=C3)NC4=CC=NC=C4

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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mg/kg

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Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
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Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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