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Tenovin-1 p53 activator

Name: Tenovin-1
Brand: Selleck Chemicals
SKU: S8000
Availability: InStock
Rating: 5 (5 reviews)

Cat.No.S8000

Tenovin-1 protects against MDM2-mediated p53 degradation, which involves ubiquitination, and acts through inhibition of protein-deacetylating activities of SirT1 and SirT2. This compound is also an inhibitor of dihydroorotate dehydrogenase (DHODH).

Chemical Structure

Molecular Weight: 369.48

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Quality Control

Batch: S800001 DMSO]250 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.82%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS
Datasheet

99.82

Related Targets	Bcl-2 Caspase PD-1/PD-L1 Ferroptosis Apoptosis related Synthetic Lethality STAT TNF-alpha Ras KRas
Other p53 Inhibitors	Eprenetapopt (APR-246) CBL0137 Hydrochloride Pifithrin-α (PFTα) Hhydrobromide Pifithrin-μ Serdemetan (JNJ-26854165) RITA PRIMA-1 NSC348884 NSC 319726 (ZMC1) Tenovin-6

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
MCF7 cells	Function assay	10 μM	6 h		Inhibition of SirT1 assessed as increase in p53 expression in human MCF7 cells at 10 uM after 6 hrs by Western blotting
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 250 mg/mL (676.62 mM) Ultrasonicated;
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	15% Captisol Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	10.000mg/ml (27.07mM)	Taking the 1 mL working solution as an example, take 10 mg of this product, add it to 1 ml of 15% Captisol clear solution, and mix evenly to form a uniform suspension. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	369.48	Formula	C₂₀H₂₃N₃O₂S	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	380315-80-0	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC(=O)NC1=CC=C(C=C1)NC(=S)NC(=O)C2=CC=C(C=C2)C(C)(C)C

Mechanism of Action

Targets/IC₅₀/Ki	p53 Mdm2 DHODH
In vitro	Tenovin-1 is a p53 activator and elevates the amount of p53 protein within 2 hr of treatment. This compound treatment does not alter p53 mRNA levels. It (10μM) protects p53 from mdm2-mediated degradation with little effect on p53 synthesis. This chemical represses cell growth, and induces apoptosis in a panel of tumor cells expressing p53. It acts through inhibition of the protein-deacetylating activities of SirT1 and SirT2, two important members of the sirtuin family.
In vivo	Initial in vivo experiments indicated that tenovin-1 impairs the growth of BL2-derived tumor xenografts.
References	[1] https://pubmed.ncbi.nlm.nih.gov/18455128/ [2] https://pubmed.ncbi.nlm.nih.gov/32900849/

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