gossypol-Acetic acid

Catalog No.S2303 Synonyms: Pogosin, AT101

gossypol-Acetic acid Chemical Structure

Molecular Weight(MW): 578.61

Gossypol Acetate is a polyphenolic aldehyde that permeates cells and acts as an inhibitor for several dehydrogenase enzymes such as lactate dehydrogenase, NAD-linked enzymes.

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Cited by 9 Publications

5 Customer Reviews

  • 16h after seeding HMLE RAS cells were exposed to 5 uM or 10 uM Gossypol in the presence or absence of Cisplatin for 16h. Afterwards, cells were harvested and lysates were analyzed for apoptosis by western blotting. Actin staining was used as loading control, while LC3a/b staining was used as indicator for autophagy. Accumulation of LC3a/b II indicates autophagy. Non-treated and DMSO-treated cells were used as control. Arrows are indicating the intended staining. For Bcl-xL: * indicates deamidated protein, # indicates unmodified protein. n=1.

    Georg August University Gottingen 2014 Western blotting. gossypol-Acetic acid purchased from Selleck.

    Weight gain of Spodoptera littoralis larvae on combined Cry proteins and gossypol. Weight gain (mg ± SE) of 2nd instar S. littoralis after feeding for one week on diet containing different concentrations of Cry proteins, gossypol, or a combination of both. Control = diet with added PBST; C1 = Cry1Ac 0.05 + Cry2Ab2 1.6 pmol/g; C3 = Cry1Ac 0.52 + Cry2Ab2 16.4 pmol/g; C4 = Cry1Ac 1.7 + Cry2Ab2 54.5 pmol/g; G3 = 1.3 μmol/g gossypol. Asterisks indicate significant difference to the control diet using a GLM with gamma distribution followed by Dunnett`s Test (n = 53-60). *** p ≤ 0.001.

    Pest Manag Sci, 2016, 73(8):1670-1678. gossypol-Acetic acid purchased from Selleck.

  • H9c2 cells were incubated with indicated bioactive compounds for 2 hours prior to protein harvest. Cells were lysed and acetyl-lysine (Ac-Lysine), acetyl-histone 3 (Ac-H3 K9/14) and total histone 3 (Total-H3) were assessed via immunoblot. Acetylation of lysine and Ac-H3 (K9/14) increased for gossypol. Acetyl proteins were normalized to total H3 and quantitation performed via Image J software.

    Mol Nutr Food Res, 2017, 61(4). gossypol-Acetic acid purchased from Selleck.

    Western blot analysis of apoptotic markers of H1975 cells after treatment of gossypol for 24 h.

    Front Pharmacol, 2018, 9:728. gossypol-Acetic acid purchased from Selleck.

  • Effect of AT-101 (AT) on MM cell lines survival. The survival of human (MM-B1, H-Meso-1, and MM-F1) and mouse (#40a) MM cell lines were assessed by the SRB assay after 24, 48, and 72 h of treatment with DMSO or AT-101. The percentage of surviving cells treated with the compound was calculated by normalizing the OD value to that of the control cultures (DMSO). The results are expressed as the means ± SD of three independent experiments performed in triplicate (xp ≤ 0.05, ∗p ≤ 0.01, #p ≤ 0.001 compared with the cultures treated with DMSO).

    Front Pharmacol, 2018, 9:1269. gossypol-Acetic acid purchased from Selleck.

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Biological Activity

Description Gossypol Acetate is a polyphenolic aldehyde that permeates cells and acts as an inhibitor for several dehydrogenase enzymes such as lactate dehydrogenase, NAD-linked enzymes.
Targets
Dehydrogenase [1] Bcl2 [5]
In vitro

Gossypol, a known antispermatogenic agent from the cotton plant genus Gossypium, was found to inhibit yellow perch sperm motility in vitro and lactate dehydrogenase activity in spermatozoa when used in a dose-dependent manner[1]. Gossypol has been approved to have antiproliferative and apoptosis-inducing effects on some kinds of cancer cell lines in vitro[2].

In vivo Gossypol acetate is able to inhibit tumor growth in Wus1-bearing mice, but the survival of mice is not prolonged, and tumor grows rapidly after short inhibition. Gossypol has now been found to have inhibitory effects on proliferation or to induce apoptosis in ovarian cancer, endometrial cancer, adrenal cortical tumor, thyroid cancer, lung cancer, colon carcinoma, leukemia, pancreatic cancer, melanoma and lymphoma. In addition, gossypol can increase the sensitivity of drug-resistant tumor cells to chemotherapy and radiotherapy. Some clinical trials showed gossypol is well-tolerated, and partial responses are observed in some patients[4].

Protocol

Cell Research:

[4]

+ Expand
  • Cell lines: Human MM cell lines U266 and Wus1
  • Concentrations: 1, 5, 10, 25 and 50 µmol/l
  • Incubation Time: 0, 24, 48 and 72 h
  • Method:

    MM(Multiple myeloma) cells are plated in 24-cell culture clusters at a density of 1x105 viable cells/l per well. Triplicate wells are treated with 1, 5, 10, 25 and 50 µmol/l gossypol acetate, and the negative control group is supplemented with 0.1% DMSO. Then, cell numbers at different treatment time points (0, 24, 48 and 72 h) are determined by using a hemocytometer and the trypan blue dye-exclusion method. The trypan blue dye-exclusion method is used to evaluate the cell viability. The cells are examined in a counting chamber under a light microscope. Only viable cells are recorded.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (172.82 mM)
Ethanol 10 mg/mL (17.28 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 578.61
Formula

C32H34O10

CAS No. 12542-36-8
Storage powder
in solvent
Synonyms Pogosin, AT101

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Frequently Asked Questions

  • Question 1:

    S2812 and S2303 share same structures. However, S2812 is described as ‘R’ enantiomer. What’s the difference between S2812 and S2303?

  • Answer:

    S2303 is the mixture of S2812 and its enantiomer.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID