Choose Your Country or Region

Axitinib

Name: Axitinib
Brand: Selleck Chemicals
SKU: S1005
Rating: 5 (185 reviews)

Synonyms: AG 013736

Catalog No.S1005 Purity:99.96%

Axitinib is a multi-target inhibitor of VEGFR1, VEGFR2, VEGFR3, PDGFRβ and c-Kit with IC50 of 0.1 nM, 0.2 nM, 0.1-0.3 nM, 1.6 nM and 1.7 nM in Porcine aorta endothelial cells, respectively.

Axitinib Chemical Structure

CAS No. 319460-85-0

Purity & Quality Control

Batch: Purity: 99.96%

99.96

Axitinib Related Products

Related Targets	VEGFR1 VEGFR2 VEGFR3	Click to Expand
Related Products	Cediranib (AZD2171) Vatalanib (PTK787) 2HCl Linifanib (ABT-869) Anlotinib (AL3818) dihydrochloride SAR131675 Apatinib Apatinib (YN968D1) mesylate Ki8751 ZM 323881 HCl Semaxanib (SU5416) SU5402 Cediranib Maleate Motesanib Diphosphate (AMG-706) Brivanib (BMS-540215) Motesanib (AMG-706) ZM 306416 KRN 633 Telatinib Fruquintinib SKLB1002 Taxifolin (Dihydroquercetin) BAW2881 (NVP-BAW2881) SU14813 SU1498 SU5614 SKLB 610 Brivanib Alaninate (BMS-582664) SU5408 SU5204	Click to Expand
Related Compound Libraries	Kinase Inhibitor Library Tyrosine Kinase Inhibitor Library PI3K/Akt Inhibitor Library Cell Cycle compound library Angiogenesis Related compound Library	Click to Expand

Signaling Pathway

VEGFR Signaling Pathway

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
EoL-1-cell	Growth Inhibition Assay		IC50=0.0000513 μM	SANGER
NCI-H1703	Growth Inhibition Assay		IC50=0.000902 μM	SANGER
KASUMI-1	Growth Inhibition Assay		IC50=0.00682 μM	SANGER
CGTH-W-1	Growth Inhibition Assay		IC50=0.00722 μM	SANGER
A204	Growth Inhibition Assay		IC50=0.00992 μM	SANGER
HOP-62	Growth Inhibition Assay		IC50=0.10836 μM	SANGER
H-EMC-SS	Growth Inhibition Assay		IC50=0.11005 μM	SANGER
KU812	Growth Inhibition Assay		IC50=0.16577 μM	SANGER
EM-2	Growth Inhibition Assay		IC50=0.16816 μM	SANGER
LAMA-84	Growth Inhibition Assay		IC50=0.17665 μM	SANGER
JAR	Growth Inhibition Assay		IC50=0.23989 μM	SANGER
G-361	Growth Inhibition Assay		IC50=0.32325 μM	SANGER
KG-1	Growth Inhibition Assay		IC50=0.37642 μM	SANGER
BV-173	Growth Inhibition Assay		IC50=0.3932 μM	SANGER
K5	Growth Inhibition Assay		IC50=0.42127 μM	SANGER
MEG-01	Growth Inhibition Assay		IC50=0.42361 μM	SANGER
MFM-223	Growth Inhibition Assay		IC50=0.44776 μM	SANGER
BE-13	Growth Inhibition Assay		IC50=0.51008 μM	SANGER
NEC8	Growth Inhibition Assay		IC50=0.72113 μM	SANGER
SW756	Growth Inhibition Assay		IC50=0.99846 μM	SANGER
A2780	Growth Inhibition Assay		IC50=1.01146 μM	SANGER
NB14	Growth Inhibition Assay		IC50=1.01801 μM	SANGER
H4	Growth Inhibition Assay		IC50=1.06422 μM	SANGER
SK-OV-3	Growth Inhibition Assay		IC50=1.06633 μM	SANGER
AN3-CA	Growth Inhibition Assay		IC50=1.08389 μM	SANGER
A427	Growth Inhibition Assay		IC50=1.10381 μM	SANGER
ES7	Growth Inhibition Assay		IC50=1.11334 μM	SANGER
AGS	Growth Inhibition Assay		IC50=1.11395 μM	SANGER
G-402	Growth Inhibition Assay		IC50=1.14694 μM	SANGER
ES5	Growth Inhibition Assay		IC50=1.17248 μM	SANGER
DEL	Growth Inhibition Assay		IC50=1.25457 μM	SANGER
NB10	Growth Inhibition Assay		IC50=1.32557 μM	SANGER
NCI-H1581	Growth Inhibition Assay		IC50=1.39042 μM	SANGER
D-566MG	Growth Inhibition Assay		IC50=1.40683 μM	SANGER
LXF-289	Growth Inhibition Assay		IC50=1.43996 μM	SANGER
BT-549	Growth Inhibition Assay		IC50=1.56909 μM	SANGER
NKM-1	Growth Inhibition Assay		IC50=1.60556 μM	SANGER
SW780	Growth Inhibition Assay		IC50=1.65158 μM	SANGER
NCI-H292	Growth Inhibition Assay		IC50=1.66383 μM	SANGER
HMV-II	Growth Inhibition Assay		IC50=1.70488 μM	SANGER
ALL-PO	Growth Inhibition Assay		IC50=1.80015 μM	SANGER
UACC-257	Growth Inhibition Assay		IC50=1.82163 μM	SANGER
PA-1	Growth Inhibition Assay		IC50=1.82725 μM	SANGER
HD-MY-Z	Growth Inhibition Assay		IC50=1.86281 μM	SANGER
HSC-4	Growth Inhibition Assay		IC50=1.93839 μM	SANGER
GCT	Growth Inhibition Assay		IC50=2.00916 μM	SANGER
RT-112	Growth Inhibition Assay		IC50=2.13424 μM	SANGER
A172	Growth Inhibition Assay		IC50=2.13606 μM	SANGER
HCE-T	Growth Inhibition Assay		IC50=2.20598 μM	SANGER
YH-13	Growth Inhibition Assay		IC50=2.21671 μM	SANGER
DK-MG	Growth Inhibition Assay		IC50=2.23834 μM	SANGER
ACN	Growth Inhibition Assay		IC50=2.23875 μM	SANGER
VA-ES-BJ	Growth Inhibition Assay		IC50=2.24957 μM	SANGER
L-363	Growth Inhibition Assay		IC50=2.28061 μM	SANGER
HuH-7	Growth Inhibition Assay		IC50=2.42165 μM	SANGER
A4-Fuk	Growth Inhibition Assay		IC50=2.47168 μM	SANGER
T-24	Growth Inhibition Assay		IC50=2.48037 μM	SANGER
GOTO	Growth Inhibition Assay		IC50=2.53013 μM	SANGER
MV-4-11	Growth Inhibition Assay		IC50=2.59169 μM	SANGER
DMS-114	Growth Inhibition Assay		IC50=2.66345 μM	SANGER
MHH-NB-11	Growth Inhibition Assay		IC50=2.70299 μM	SANGER
CHP-212	Growth Inhibition Assay		IC50=2.82091 μM	SANGER
DMS-273	Growth Inhibition Assay		IC50=2.90207 μM	SANGER
SF295	Growth Inhibition Assay		IC50=3.02597 μM	SANGER
NCI-H1563	Growth Inhibition Assay		IC50=3.15005 μM	SANGER
NCI-H446	Growth Inhibition Assay		IC50=3.22805 μM	SANGER
HCC1806	Growth Inhibition Assay		IC50=3.27657 μM	SANGER
SF126	Growth Inhibition Assay		IC50=3.30017 μM	SANGER
SW982	Growth Inhibition Assay		IC50=3.33875 μM	SANGER
ES8	Growth Inhibition Assay		IC50=3.34999 μM	SANGER
SCC-4	Growth Inhibition Assay		IC50=3.50396 μM	SANGER
RPMI-8226	Growth Inhibition Assay		IC50=3.62616 μM	SANGER
EW-11	Growth Inhibition Assay		IC50=3.63022 μM	SANGER
COR-L105	Growth Inhibition Assay		IC50=3.63334 μM	SANGER
ES1	Growth Inhibition Assay		IC50=3.83094 μM	SANGER
KMOE-2	Growth Inhibition Assay		IC50=3.91808 μM	SANGER
ABC-1	Growth Inhibition Assay		IC50=3.93911 μM	SANGER
NCI-H526	Growth Inhibition Assay		IC50=3.99126 μM	SANGER
HCC1395	Growth Inhibition Assay		IC50=3.99484 μM	SANGER
DU-145	Growth Inhibition Assay		IC50=4.12825 μM	SANGER
JEG-3	Growth Inhibition Assay		IC50=4.15916 μM	SANGER
HCC1187	Growth Inhibition Assay		IC50=4.21597 μM	SANGER
LC-2-ad	Growth Inhibition Assay		IC50=4.22177 μM	SANGER
ONS-76	Growth Inhibition Assay		IC50=4.24192 μM	SANGER
CAL-27	Growth Inhibition Assay		IC50=4.24344 μM	SANGER
8-MG-BA	Growth Inhibition Assay		IC50=4.26658 μM	SANGER
HGC-27	Growth Inhibition Assay		IC50=4.2966 μM	SANGER
Hs-578-T	Growth Inhibition Assay		IC50=4.31468 μM	SANGER
EW-1	Growth Inhibition Assay		IC50=4.53014 μM	SANGER
SW1573	Growth Inhibition Assay		IC50=4.55163 μM	SANGER
SNU-423	Growth Inhibition Assay		IC50=4.6079 μM	SANGER
HOS	Growth Inhibition Assay		IC50=4.6977 μM	SANGER
LB1047-RCC	Growth Inhibition Assay		IC50=4.81418 μM	SANGER
ChaGo-K-1	Growth Inhibition Assay		IC50=4.89048 μM	SANGER
A3-KAW	Growth Inhibition Assay		IC50=4.97352 μM	SANGER
CAS-1	Growth Inhibition Assay		IC50=4.99908 μM	SANGER
NBsusSR	Growth Inhibition Assay		IC50=5.03514 μM	SANGER
KM12	Growth Inhibition Assay		IC50=5.29827 μM	SANGER
NCI-H1155	Growth Inhibition Assay		IC50=5.38185 μM	SANGER
EFM-19	Growth Inhibition Assay		IC50=5.41737 μM	SANGER
D-392MG	Growth Inhibition Assay		IC50=5.57849 μM	SANGER
JVM-3	Growth Inhibition Assay		IC50=5.72325 μM	SANGER
EW-16	Growth Inhibition Assay		IC50=5.75583 μM	SANGER
KARPAS-45	Growth Inhibition Assay		IC50=5.84325 μM	SANGER
NCI-H28	Growth Inhibition Assay		IC50=5.87918 μM	SANGER
COLO-829	Growth Inhibition Assay		IC50=5.91504 μM	SANGER
KM-H2	Growth Inhibition Assay		IC50=5.92395 μM	SANGER
NCI-H82	Growth Inhibition Assay		IC50=5.92771 μM	SANGER
OAW-42	Growth Inhibition Assay		IC50=5.98821 μM	SANGER
A704	Growth Inhibition Assay		IC50=6.10574 μM	SANGER
NCI-H1048	Growth Inhibition Assay		IC50=6.10599 μM	SANGER
LOXIMVI	Growth Inhibition Assay		IC50=6.11248 μM	SANGER
MKN45	Growth Inhibition Assay		IC50=6.26016 μM	SANGER
D-502MG	Growth Inhibition Assay		IC50=6.28857 μM	SANGER
HUTU-80	Growth Inhibition Assay		IC50=6.41688 μM	SANGER
S-117	Growth Inhibition Assay		IC50=6.50267 μM	SANGER
HCC1569	Growth Inhibition Assay		IC50=6.53737 μM	SANGER
J-RT3-T3-5	Growth Inhibition Assay		IC50=6.54572 μM	SANGER
OC-314	Growth Inhibition Assay		IC50=6.91159 μM	SANGER
SNU-449	Growth Inhibition Assay		IC50=7.01072 μM	SANGER
NCI-H720	Growth Inhibition Assay		IC50=7.19345 μM	SANGER
KP-N-YS	Growth Inhibition Assay		IC50=7.2072 μM	SANGER
IGROV-1	Growth Inhibition Assay		IC50=7.32386 μM	SANGER
SK-PN-DW	Growth Inhibition Assay		IC50=7.4815 μM	SANGER
HCC1419	Growth Inhibition Assay		IC50=7.53 μM	SANGER
HAL-01	Growth Inhibition Assay		IC50=7.60644 μM	SANGER
HCC2998	Growth Inhibition Assay		IC50=7.60743 μM	SANGER
SK-N-FI	Growth Inhibition Assay		IC50=7.63033 μM	SANGER
GI-ME-N	Growth Inhibition Assay		IC50=7.64934 μM	SANGER
SW1088	Growth Inhibition Assay		IC50=7.65826 μM	SANGER
IA-LM	Growth Inhibition Assay		IC50=7.68613 μM	SANGER
SK-NEP-1	Growth Inhibition Assay		IC50=7.6961 μM	SANGER
MDA-MB-415	Growth Inhibition Assay		IC50=7.89186 μM	SANGER
COLO-800	Growth Inhibition Assay		IC50=7.94492 μM	SANGER
NCI-H2228	Growth Inhibition Assay		IC50=8.15783 μM	SANGER
D-423MG	Growth Inhibition Assay		IC50=8.2172 μM	SANGER
TE-1	Growth Inhibition Assay		IC50=8.44316 μM	SANGER
NOS-1	Growth Inhibition Assay		IC50=8.51534 μM	SANGER
8505C	Growth Inhibition Assay		IC50=8.64824 μM	SANGER
HEC-1	Growth Inhibition Assay		IC50=8.78439 μM	SANGER
TE-11	Growth Inhibition Assay		IC50=8.99551 μM	SANGER
CTB-1	Growth Inhibition Assay		IC50=9.01433 μM	SANGER
TGBC11TKB	Growth Inhibition Assay		IC50=9.02241 μM	SANGER
NB17	Growth Inhibition Assay		IC50=9.1887 μM	SANGER
Becker	Growth Inhibition Assay		IC50=9.41944 μM	SANGER
SN12C	Growth Inhibition Assay		IC50=9.45234 μM	SANGER
COLO-320-HSR	Growth Inhibition Assay		IC50=9.60237 μM	SANGER
D-283MED	Growth Inhibition Assay		IC50=9.63072 μM	SANGER
D-263MG	Growth Inhibition Assay		IC50=9.83384 μM	SANGER
MEL-JUSO	Growth Inhibition Assay		IC50=9.90127 μM	SANGER
T98G	Growth Inhibition Assay		IC50=9.90203 μM	SANGER
HLE	Growth Inhibition Assay		IC50=9.90909 μM	SANGER
Ca9-22	Growth Inhibition Assay		IC50=10.0665 μM	SANGER
OS-RC-2	Growth Inhibition Assay		IC50=10.1045 μM	SANGER
T47D	Growth Inhibition Assay		IC50=10.155 μM	SANGER
GI-1	Growth Inhibition Assay		IC50=10.3533 μM	SANGER
NUGC-3	Growth Inhibition Assay		IC50=10.4402 μM	SANGER
MDA-MB-361	Growth Inhibition Assay		IC50=10.4432 μM	SANGER
SCC-15	Growth Inhibition Assay		IC50=10.4718 μM	SANGER
KS-1	Growth Inhibition Assay		IC50=10.6301 μM	SANGER
CAL-12T	Growth Inhibition Assay		IC50=10.6361 μM	SANGER
OVCAR-4	Growth Inhibition Assay		IC50=10.7068 μM	SANGER
HuP-T4	Growth Inhibition Assay		IC50=11.0328 μM	SANGER
NCI-H358	Growth Inhibition Assay		IC50=11.2657 μM	SANGER
HO-1-N-1	Growth Inhibition Assay		IC50=11.3398 μM	SANGER
NH-12	Growth Inhibition Assay		IC50=11.5378 μM	SANGER
MOLT-4	Growth Inhibition Assay		IC50=11.5985 μM	SANGER
K-562	Growth Inhibition Assay		IC50=11.7248 μM	SANGER
ES6	Growth Inhibition Assay		IC50=11.8581 μM	SANGER
RO82-W-1	Growth Inhibition Assay		IC50=11.9064 μM	SANGER
Ramos-2G6-4C10	Growth Inhibition Assay		IC50=11.932 μM	SANGER
23132-87	Growth Inhibition Assay		IC50=12.0821 μM	SANGER
A549	Growth Inhibition Assay		IC50=12.3285 μM	SANGER
NCI-H23	Growth Inhibition Assay		IC50=12.5026 μM	SANGER
H9	Growth Inhibition Assay		IC50=12.5577 μM	SANGER
LB771-HNC	Growth Inhibition Assay		IC50=12.765 μM	SANGER
QIMR-WIL	Growth Inhibition Assay		IC50=12.8258 μM	SANGER
HSC-3	Growth Inhibition Assay		IC50=12.9276 μM	SANGER
PFSK-1	Growth Inhibition Assay		IC50=12.9507 μM	SANGER
ETK-1	Growth Inhibition Assay		IC50=13.0779 μM	SANGER
SW1710	Growth Inhibition Assay		IC50=13.3641 μM	SANGER
COLO-684	Growth Inhibition Assay		IC50=13.4541 μM	SANGER
RPMI-7951	Growth Inhibition Assay		IC50=13.5136 μM	SANGER
A101D	Growth Inhibition Assay		IC50=13.5349 μM	SANGER
KE-37	Growth Inhibition Assay		IC50=13.5887 μM	SANGER
SiHa	Growth Inhibition Assay		IC50=13.8346 μM	SANGER
NCI-H226	Growth Inhibition Assay		IC50=13.8808 μM	SANGER
DB	Growth Inhibition Assay		IC50=13.9928 μM	SANGER
HT-1197	Growth Inhibition Assay		IC50=14.0809 μM	SANGER
SBC-5	Growth Inhibition Assay		IC50=14.1362 μM	SANGER
VMRC-RCZ	Growth Inhibition Assay		IC50=14.5774 μM	SANGER
697	Growth Inhibition Assay		IC50=14.627 μM	SANGER
OMC-1	Growth Inhibition Assay		IC50=14.7888 μM	SANGER
SKG-IIIa	Growth Inhibition Assay		IC50=14.8001 μM	SANGER
DOK	Growth Inhibition Assay		IC50=14.9933 μM	SANGER
NCI-H2029	Growth Inhibition Assay		IC50=15.3602 μM	SANGER
NCI-H2009	Growth Inhibition Assay		IC50=15.5095 μM	SANGER
LK-2	Growth Inhibition Assay		IC50=15.6449 μM	SANGER
NCI-H661	Growth Inhibition Assay		IC50=15.9075 μM	SANGER
GT3TKB	Growth Inhibition Assay		IC50=16.0676 μM	SANGER
GP5d	Growth Inhibition Assay		IC50=16.342 μM	SANGER
SK-MEL-2	Growth Inhibition Assay		IC50=16.4485 μM	SANGER
SK-UT-1	Growth Inhibition Assay		IC50=16.565 μM	SANGER
NB7	Growth Inhibition Assay		IC50=16.6971 μM	SANGER
NCI-H460	Growth Inhibition Assay		IC50=16.7326 μM	SANGER
8305C	Growth Inhibition Assay		IC50=16.7877 μM	SANGER
CaR-1	Growth Inhibition Assay		IC50=16.8111 μM	SANGER
D-247MG	Growth Inhibition Assay		IC50=16.8625 μM	SANGER
LoVo	Growth Inhibition Assay		IC50=16.9488 μM	SANGER
NCI-H2405	Growth Inhibition Assay		IC50=17.1908 μM	SANGER
AU565	Growth Inhibition Assay		IC50=17.225 μM	SANGER
OCI-AML2	Growth Inhibition Assay		IC50=17.5317 μM	SANGER
22RV1	Growth Inhibition Assay		IC50=17.5884 μM	SANGER
HT-144	Growth Inhibition Assay		IC50=17.6598 μM	SANGER
HuO9	Growth Inhibition Assay		IC50=17.7031 μM	SANGER
Daoy	Growth Inhibition Assay		IC50=17.7184 μM	SANGER
SJRH30	Growth Inhibition Assay		IC50=17.8489 μM	SANGER
CHL-1	Growth Inhibition Assay		IC50=17.9249 μM	SANGER
J82	Growth Inhibition Assay		IC50=17.9635 μM	SANGER
COR-L23	Growth Inhibition Assay		IC50=18.0011 μM	SANGER
SNU-C2B	Growth Inhibition Assay		IC50=18.2276 μM	SANGER
NCI-H1770	Growth Inhibition Assay		IC50=18.4615 μM	SANGER
MHH-PREB-1	Growth Inhibition Assay		IC50=18.5697 μM	SANGER
ES3	Growth Inhibition Assay		IC50=18.5883 μM	SANGER
MDA-MB-231	Growth Inhibition Assay		IC50=18.6502 μM	SANGER
MN-60	Growth Inhibition Assay		IC50=19.0592 μM	SANGER
EPLC-272H	Growth Inhibition Assay		IC50=19.3708 μM	SANGER
SW948	Growth Inhibition Assay		IC50=19.3934 μM	SANGER
MOLT-13	Growth Inhibition Assay		IC50=19.4546 μM	SANGER
HL-60	Growth Inhibition Assay		IC50=20.2141 μM	SANGER
CP50-MEL-B	Growth Inhibition Assay		IC50=20.4748 μM	SANGER
NTERA-S-cl-D1	Growth Inhibition Assay		IC50=20.4978 μM	SANGER
KINGS-1	Growth Inhibition Assay		IC50=20.7967 μM	SANGER
DOHH-2	Growth Inhibition Assay		IC50=20.906 μM	SANGER
BB65-RCC	Growth Inhibition Assay		IC50=20.9285 μM	SANGER
NB12	Growth Inhibition Assay		IC50=21.0394 μM	SANGER
KY821	Growth Inhibition Assay		IC50=21.582 μM	SANGER
PSN1	Growth Inhibition Assay		IC50=21.6453 μM	SANGER
EGI-1	Growth Inhibition Assay		IC50=21.7454 μM	SANGER
CTV-1	Growth Inhibition Assay		IC50=22.3031 μM	SANGER
TI-73	Growth Inhibition Assay		IC50=22.3498 μM	SANGER
LCLC-103H	Growth Inhibition Assay		IC50=22.4752 μM	SANGER
D-542MG	Growth Inhibition Assay		IC50=22.5558 μM	SANGER
ATN-1	Growth Inhibition Assay		IC50=22.6439 μM	SANGER
SK-MEL-1	Growth Inhibition Assay		IC50=22.8368 μM	SANGER
HDLM-2	Growth Inhibition Assay		IC50=23.1478 μM	SANGER
UM-UC-3	Growth Inhibition Assay		IC50=23.1944 μM	SANGER
NCI-H1573	Growth Inhibition Assay		IC50=23.4681 μM	SANGER
NCI-H520	Growth Inhibition Assay		IC50=23.4948 μM	SANGER
ESS-1	Growth Inhibition Assay		IC50=23.8059 μM	SANGER
COR-L88	Growth Inhibition Assay		IC50=23.9475 μM	SANGER
TGBC24TKB	Growth Inhibition Assay		IC50=24.0312 μM	SANGER
HCC1937	Growth Inhibition Assay		IC50=24.1 μM	SANGER
RS4-11	Growth Inhibition Assay		IC50=24.142 μM	SANGER
HCC38	Growth Inhibition Assay		IC50=24.2394 μM	SANGER
RPMI-2650	Growth Inhibition Assay		IC50=24.6162 μM	SANGER
P12-ICHIKAWA	Growth Inhibition Assay		IC50=24.6258 μM	SANGER
YAPC	Growth Inhibition Assay		IC50=24.8214 μM	SANGER
NB13	Growth Inhibition Assay		IC50=25.2611 μM	SANGER
SK-N-AS	Growth Inhibition Assay		IC50=25.8584 μM	SANGER
SK-N-DZ	Growth Inhibition Assay		IC50=26.049 μM	SANGER
LS-411N	Growth Inhibition Assay		IC50=26.2038 μM	SANGER
NCI-H810	Growth Inhibition Assay		IC50=26.3112 μM	SANGER
NCI-SNU-1	Growth Inhibition Assay		IC50=26.5454 μM	SANGER
HH	Growth Inhibition Assay		IC50=26.5529 μM	SANGER
U-2-OS	Growth Inhibition Assay		IC50=26.7382 μM	SANGER
SF539	Growth Inhibition Assay		IC50=26.8018 μM	SANGER
NCI-H2052	Growth Inhibition Assay		IC50=27.086 μM	SANGER
A673	Growth Inhibition Assay		IC50=27.2102 μM	SANGER
WM-115	Growth Inhibition Assay		IC50=27.7767 μM	SANGER
SW48	Growth Inhibition Assay		IC50=27.8005 μM	SANGER
NOMO-1	Growth Inhibition Assay		IC50=27.8532 μM	SANGER
PC-3	Growth Inhibition Assay		IC50=27.8941 μM	SANGER
UMC-11	Growth Inhibition Assay		IC50=27.9343 μM	SANGER
U-118-MG	Growth Inhibition Assay		IC50=28.0123 μM	SANGER
NCI-H2452	Growth Inhibition Assay		IC50=28.0822 μM	SANGER
CAMA-1	Growth Inhibition Assay		IC50=28.8564 μM	SANGER
MC-IXC	Growth Inhibition Assay		IC50=29.2366 μM	SANGER
ES4	Growth Inhibition Assay		IC50=29.3172 μM	SANGER
BHT-101	Growth Inhibition Assay		IC50=29.321 μM	SANGER
KP-4	Growth Inhibition Assay		IC50=29.516 μM	SANGER
CAL-54	Growth Inhibition Assay		IC50=29.5445 μM	SANGER
5637	Growth Inhibition Assay		IC50=29.6421 μM	SANGER
MOLT-16	Growth Inhibition Assay		IC50=29.7269 μM	SANGER
Ca-Ski	Growth Inhibition Assay		IC50=29.946 μM	SANGER
AsPC-1	Growth Inhibition Assay		IC50=30.0212 μM	SANGER
MSTO-211H	Growth Inhibition Assay		IC50=30.15 μM	SANGER
L-428	Growth Inhibition Assay		IC50=30.405 μM	SANGER
SW1463	Growth Inhibition Assay		IC50=30.5383 μM	SANGER
NCI-H1648	Growth Inhibition Assay		IC50=30.5574 μM	SANGER
CAKI-1	Growth Inhibition Assay		IC50=30.7702 μM	SANGER
YKG-1	Growth Inhibition Assay		IC50=31.0263 μM	SANGER
A2058	Growth Inhibition Assay		IC50=31.1164 μM	SANGER
A375	Growth Inhibition Assay		IC50=31.1696 μM	SANGER
SNB75	Growth Inhibition Assay		IC50=31.2435 μM	SANGER
SK-HEP-1	Growth Inhibition Assay		IC50=31.4271 μM	SANGER
ME-180	Growth Inhibition Assay		IC50=31.6542 μM	SANGER
NCI-H209	Growth Inhibition Assay		IC50=31.8247 μM	SANGER
HC-1	Growth Inhibition Assay		IC50=32.1446 μM	SANGER
LB373-MEL-D	Growth Inhibition Assay		IC50=32.1971 μM	SANGER
SNU-387	Growth Inhibition Assay		IC50=32.3191 μM	SANGER
C32	Growth Inhibition Assay		IC50=32.3353 μM	SANGER
EW-13	Growth Inhibition Assay		IC50=32.9408 μM	SANGER
BFTC-905	Growth Inhibition Assay		IC50=33.5136 μM	SANGER
NCI-H1299	Growth Inhibition Assay		IC50=33.5621 μM	SANGER
LU-135	Growth Inhibition Assay		IC50=33.801 μM	SANGER
NCI-H2122	Growth Inhibition Assay		IC50=33.9966 μM	SANGER
SK-LMS-1	Growth Inhibition Assay		IC50=34.4107 μM	SANGER
LNCaP-Clone-FGC	Growth Inhibition Assay		IC50=34.8515 μM	SANGER
NCI-H1092	Growth Inhibition Assay		IC50=35.2747 μM	SANGER
MS-1	Growth Inhibition Assay		IC50=35.3038 μM	SANGER
KYSE-510	Growth Inhibition Assay		IC50=35.5042 μM	SANGER
NCI-H1793	Growth Inhibition Assay		IC50=35.6545 μM	SANGER
MIA-PaCa-2	Growth Inhibition Assay		IC50=36.0496 μM	SANGER
EW-22	Growth Inhibition Assay		IC50=36.4072 μM	SANGER
IGR-1	Growth Inhibition Assay		IC50=36.8184 μM	SANGER
HT-1080	Growth Inhibition Assay		IC50=37.125 μM	SANGER
M14	Growth Inhibition Assay		IC50=37.1642 μM	SANGER
786-0	Growth Inhibition Assay		IC50=37.2794 μM	SANGER
MZ2-MEL	Growth Inhibition Assay		IC50=37.4501 μM	SANGER
NCI-H510A	Growth Inhibition Assay		IC50=37.9412 μM	SANGER
LAN-6	Growth Inhibition Assay		IC50=37.9582 μM	SANGER
SW620	Growth Inhibition Assay		IC50=38.4974 μM	SANGER
LB2241-RCC	Growth Inhibition Assay		IC50=39.8205 μM	SANGER
Detroit562	Growth Inhibition Assay		IC50=40.1268 μM	SANGER
HN	Growth Inhibition Assay		IC50=40.1782 μM	SANGER
HCT-15	Growth Inhibition Assay		IC50=40.5907 μM	SANGER
C2BBe1	Growth Inhibition Assay		IC50=40.9157 μM	SANGER
A498	Growth Inhibition Assay		IC50=41.3015 μM	SANGER
SK-MEL-24	Growth Inhibition Assay		IC50=41.4725 μM	SANGER
OVCAR-5	Growth Inhibition Assay		IC50=41.7767 μM	SANGER
NCI-H1792	Growth Inhibition Assay		IC50=41.9821 μM	SANGER
KOSC-2	Growth Inhibition Assay		IC50=42.2699 μM	SANGER
Mo-T	Growth Inhibition Assay		IC50=42.8958 μM	SANGER
CFPAC-1	Growth Inhibition Assay		IC50=43.4944 μM	SANGER
CAL-51	Growth Inhibition Assay		IC50=43.5605 μM	SANGER
RH-18	Growth Inhibition Assay		IC50=43.805 μM	SANGER
EC-GI-10	Growth Inhibition Assay		IC50=43.8347 μM	SANGER
HSC-2	Growth Inhibition Assay		IC50=44.009 μM	SANGER
ML-2	Growth Inhibition Assay		IC50=45.2621 μM	SANGER
KNS-81-FD	Growth Inhibition Assay		IC50=45.7365 μM	SANGER
NB6	Growth Inhibition Assay		IC50=46.111 μM	SANGER
MCF7	Growth Inhibition Assay		IC50=46.5533 μM	SANGER
P30-OHK	Growth Inhibition Assay		IC50=46.8117 μM	SANGER
BPH-1	Growth Inhibition Assay		IC50=46.9805 μM	SANGER
U251	Growth Inhibition Assay		IC50=46.994 μM	SANGER
MKN1	Growth Inhibition Assay		IC50=47.5137 μM	SANGER
A431	Growth Inhibition Assay		IC50=47.8338 μM	SANGER
C8166	Growth Inhibition Assay		IC50=49.2039 μM	SANGER
HEL	Growth Inhibition Assay		IC50=49.4064 μM	SANGER
RMG-I	Growth Inhibition Assay		IC50=49.4444 μM	SANGER
CAL-72	Growth Inhibition Assay		IC50=49.6075 μM	SANGER
SW962	Growth Inhibition Assay		IC50=49.9332 μM	SANGER
MCF7	Antiproliferative assay	5 days	Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 5 days by SRB assay, GI50 = 0.97 μM.	23829549
MDA-MB-468	Antiproliferative assay	5 days	Antiproliferative activity against human MDA-MB-468 cells assessed as growth inhibition after 5 days by SRB assay, GI50 = 1.3 μM.	23829549
HCC1954	Antiproliferative assay	5 days	Antiproliferative activity against human HCC1954 cells assessed as growth inhibition after 5 days by SRB assay, GI50 = 2.7 μM.	23829549
SKBR3	Antiproliferative assay	5 days	Antiproliferative activity against human SKBR3 cells assessed as growth inhibition after 5 days by SRB assay, GI50 = 3.8 μM.	23829549
MDA-MB-231	Antiproliferative assay	5 days	Antiproliferative activity against human MDA-MB-231 cells assessed as growth inhibition after 5 days by SRB assay, GI50 = 7.3 μM.	23829549
MCF7	Cytotoxicity assay	5 days	Cytotoxicity against human MCF7 cells after 5 days by SRB assay, GI50 = 2.3 μM.	24867403
MDA-MB-468	Cytotoxicity assay	5 days	Cytotoxicity against human MDA-MB-468 cells after 5 days by SRB assay, GI50 = 2.8 μM.	24867403
SKBR3	Cytotoxicity assay	5 days	Cytotoxicity against human SKBR3 cells after 5 days by SRB assay, GI50 = 4.6 μM.	24867403
MDA-MB-231	Cytotoxicity assay	5 days	Cytotoxicity against human MDA-MB-231 cells after 5 days by SRB assay, GI50 = 11 μM.	24867403
Saos-2	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells	29435139
BT-12	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells	29435139
OHS-50	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells	29435139
SJ-GBM2	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells	29435139
SK-N-MC	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
NB-EBc1	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells	29435139
Rh18	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh18 cells	29435139
HUVEC	Antiproliferative assay	1 hr	Antiproliferative activity against VEGF-stimulated HUVEC cells assessed as reduction in cell viability preincubated for 1 hr followed by addition of VEGF measured after 72 hrs by MTT assay, IC50 = 0.0005 μM.	ChEMBL
HUVEC	Antiproliferative assay	1 hr	Antiproliferative activity against VEGF-stimulated HUVEC cells assessed as reduction in cell viability preincubated for 1 hr followed by addition of VEGF measured after 72 hrs in presence of albumin by MTT assay, IC50 = 0.0027 μM.	ChEMBL
A549	Antiproliferative assay	48 hrs	Antiproliferative activity against VEGF-stimulated human A549 cells after 48 hrs by CCK8 assay, IC50 = 22.4 μM.	ChEMBL
HepG2	Antiproliferative assay	48 hrs	Antiproliferative activity against VEGF-stimulated human HepG2 cells after 48 hrs by CCK8 assay, IC50 = 38.7 μM.	ChEMBL
Click to View More Cell Line Experimental Data

Biological Activity

Description

Features

Superior as second-line therapy relative to sorafenib (current standard-of-care).

Targets

VEGFR1/FLT1 ^[1] (Porcine aorta endothelial cells)	VEGFR2/Flk1 ^[1] (Porcine aorta endothelial cells)	VEGFR2/KDR ^[1] (Porcine aorta endothelial cells)	VEGFR3 ^[1] (Porcine aorta endothelial cells)	PDGFRβ ^[1] (Porcine aorta endothelial cells)	Click to View More Targets
0.1 nM	0.18 nM	0.2 nM	0.1 nM-0.3 nM	1.6 nM	Click to View More Targets

In vitro
In vitro	Axitinib could block the cellular autophosphorylation of VEGFR and VEGF-mediated endothelial cell viability, tube formation, and downstream signaling. Axitinib inhibits the proliferation of variable cell lines with IC50 of >10,000 nM (IGR-N91), 849 nM (IGR-NB8), 274 nM (SH-SY5Y) and 573 nM (non-VEGF stimulated HUVEC). ^[2]
Kinase Assay	Cellular receptor kinase phosphorylation assay
Kinase Assay	Porcine aorta endothelial (PAE) cells, which overexpress full-length VEGFR2, PDGFRβ, Kit, and NIH-3T3, which overexpress murine VEGFR2 (Flk-1) or PDGFRα, are generated. The 96-well plates are coated with 100 μL/well of 2.5 μg/mL anti-VEGFR2 antibody, 0.75 μg/mL anti-PDGFRβ antibody, 0.25 μg/mL anti-PDGFRα antibody, 0.5 μg/mL anti-KIT antibody, or 1.20 μg/mL anti-Flk-1 antibody to prepare ELISA capture plates. Then phosphorylation of RTK is measured by ELISA.
Cell Research	Cell lines	HUVEC, SH-SY5Y, IGR-N91 and IGR-NB8 cells
	Concentrations	1 nM - 10 μM
	Incubation Time	72 hours
	Method	Cells are seeded in a 96-well plate at a density of 5 × 10⁴ and cultured for 24 hours. Axitinib is added to the cells at concentrations ranging from 1 nM to 10 μM. Cell viability is measured after 72 hours by MTS tetrazolium substrate and IC50 values are calculated.
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	p-c-kit / c-kit / p-AKT / AKT / p-ERK / ERK / p-STAT3 / STAT3 / p-STAT5 / STAT5 / p-S6K / S6K / p-S6 / S6 PARP / cleaved PARP / caspase-3 / cleaved caspase-3		31205508
	Immunofluorescence	alpha-tubulin		26474283
	Growth inhibition assay	Cell viability		26474283

In Vivo
In vivo	Axitinib exhibits primary inhibition to orthotopically transplanted models such as M24met (melanoma), HCT-116 (colorectal cancer), and SN12C (renal cell carcinoma). ^[1] Axitinib delays the tumor growth with 11.4 days compared to the controls (p.o. 30 mg/kg) and decreases the Mean Vessels Density (MVD) to 21, compared to 49 in controls, in IGR-N91 ﬂank xenografts. ^[2] Axitinib significantly inhibits growth and disrupts tumor microvasculature in BT474 breast cancer model at 10-100 mg/kg. ^[3] Axitinib has shown single-agent activity in variable tumors, including renal cell carcinoma, thyroid cancer, non-small cell lung cancer, and melanoma.
Animal Research	Animal Models	BT474 breast cancer cells are implanted subcutaneously into Immune-deficient female mice (Nu/nu; age 8-12 weeks).
	Dosages	10, 30 or 100 mg/kg
	Administration	Oral daily

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT06161558	Not yet recruiting	Neoplasms	National Cancer Institute (NCI)\|National Institutes of Health Clinical Center (CC)	May 15 2024	Phase 1
NCT05949632	Recruiting	Advanced Solid Tumors	Incyte Corporation	April 16 2024	Phase 1\|Phase 2
NCT05969496	Recruiting	Renal Cancer\|Kidney Cancer\|Renal Cell Carcinoma\|Inferior Vena Cava Thrombosis	University of Colorado Denver\|Cancer League of Colorado	December 4 2023	Phase 2
NCT05904730	Enrolling by invitation	Breast Cancer\|Ovarian Cancer\|Breast Neoplasms\|Ovarian Neoplasms\|BRCA Mutation\|HER2-positive Breast Cancer\|HER2-negative Breast Cancer\|HER-2 Protein Overexpression	Lynkcell Europe	July 11 2023	Phase 1
NCT05817903	Recruiting	Metastatic Renal Cell Carcinoma	Consorzio Oncotech\|Pfizer	April 18 2023	Phase 2
NCT05263609	Withdrawn	Renal Cell Cancer	University of Oklahoma\|Merck Sharp & Dohme LLC\|Pfizer	June 2022	Phase 2

References

+ Expand

Chemical Information & Solubility

Molecular Weight	386.47	Formula	C₂₂H₁₈N₄OS
CAS No.	319460-85-0	SDF	Download Axitinib SDF
Smiles	CNC(=O)C1=CC=CC=C1SC2=CC3=C(C=C2)C(=NN3)C=CC4=CC=CC=N4
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 26 mg/mL ( (67.27 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5% DMSO 1 40% PEG 300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.850mg/ml (4.79mM)	Taking the 1 mL working solution as an example, add 50 μL of 37 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.200mg/ml (0.52mM)	Taking the 1 mL working solution as an example, add 50 μL of 4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Frequently Asked Questions

Question 1:
We would like to store at -80℃ after it dissolved in CMC. Do you have any infomation about the suggested concentration in CMC?

Answer:
Axitinib in 0.5% CMC at up to 30mg/ml is a suspension for oral gavage administration only. We don't suggest customer to freeze down the solution but to make fresh if possible. Customer can store it for 1-2 weeks at 4 degree for continuous use.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):