Name: Acyclovir (Aciclovir)
Brand: Selleck Chemicals
SKU: S1807
Availability: InStock
Rating: 5 (20 reviews)

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Quality Control

Batch: Purity: 99.93%

99.93

Related Targets	Integrase Bacterial Antibiotics Anti-infection Fungal COVID-19 Parasite Reverse Transcriptase HIV HCV Protease
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Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Incubation Time	Activity Description
BSC-1 cells	Function assay		Antiviral activity of the compound was evaluated against the Herpes simplex virus type-1 in BSC-1 cells, IC50=2.6 μM
P3HR-1 cells	Function assay		Effective concentration for the inhibition of Epstein-Barr virus EBV-DNA synthesis in human lymphoblastoid P3HR-1 cells, EC50=6.75 μM
HFF cells	Function assay		Concentration for HSV-1 plaque reduction (VPR) by 50% in HFF cells, EC50=1.1 μM
HFF cells	Function assay		Inhibitory concentration required to reduce HSV-1 induced cytopathogenic effect (CPE) by 50 % in HFF cells, EC50=2.22 μM
human embryonic lung cells	Function assay		Antiviral activity was measured as effective concentration required to reduce Varicella Zoster virus (OKA)-induced plaque formation in human embryonic lung cells, EC50=1.1 μM
HEL cells	Function assay		Compound was tested for anti-viral activity against HSV-1(G) in HEL cells, EC50=1.3 μM
HEL cell	Function assay		Effective concentration required to inhibit Tyrosine kinase (TK+) Varicella-Zoster virus-induced cytopathicity by 50% in OKA strain HEL cell lines, EC50=4.53 μM
HSV-2 MS Vero cells	Function assay		Inhibition of viral cytopathic effect in infected human foreskin fibroblast cell monolayers of HSV-2 MS Vero cells by 50%, EC50=6.2 μM
HSV-1 E-377 Vero cells	Function assay		Inhibition of plaque formation in monolayers of HSV-1 E-377 Vero cells by 50%, EC50=4.4 μM
HSV-1 KOS Vero cells	Function assay		Inhibition of plaque formation in monolayers of HSV-1 KOS Vero cells by 50%, EC50=2.2 μM
HeLa cell	Function assay		Ability to inhibit cytopathogenicity of herpes simplex type 1 virus (G) in HeLa cell culture, IC50=0.19 μM
HEp-2 cells	Function assay		Minimum inhibitory concentration causing 25% inhibition of cytopathic effect induced by Herpes simplex virus-1 (K979) in HEp-2 cells
HeLa cells	Function assay		Inhibition of HSV-1 DNA synthesis in virus-infected HeLa cells, IC50=1.9 μM
human HFF cells	Function assay		Inhibitory concentration of the drug against the cytopathic effect for E-377 strain of herpes simplex virus-1 (HSV-1) in human HFF cells, EC50=0.04 μM
human HFF cells	Function assay		Inhibitory concentration of the drug against the cytopathic effect for MS strain of herpes simplex virus-2 (HSV-2) in human HFF cells, EC50=0.09 μM
MRC-5 cells	Function assay		Antiviral activity in plaque reduction assay was determined against herpes simplex virus type 2 (HSV-2) in MRC-5 cells, IC50=2.5 μM
human lung fibroblasts (MRC-5)	Function assay		Compound was tested for antiviral activity against Herpes Simplex virus Type-1(18189) in human lung fibroblasts (MRC-5)
Raji cells	Function assay		Inhibitory concentration of the drug against the antigen production against P3HR-1 strain of epstein barr virus-2 (EBV) in Raji cells, EC50=2.9 μM
Vero cells	Function assay		Inhibitory activity against herpes simplex virus type 2 (HSV 2) strain 186 in Vero cells, IC50=1.7 μM
BSC-1 cells	Function assay		Effective concentration required to inhibit herpes simplex virus 1 in BSC-1 cells in ELISA, EC50=1.5 μM
HFF cells	Function assay		Effective concentration required to inhibit varicella zoster virus replication in HFF cells, EC50=1.6 μM
HFF cells	Function assay		Effective concentration required to inhibit herpes simplex virus 1 in HFF cells in cytopathic effect (CPE) assay, EC50=0.9 μM
Daudi cells	Function assay		Inhibition of EBV replication in Daudi cells by viral capsid antigen-ELISA, EC50=0.33 μM
african green monkey Vero cells	Function assay	48 h	Antiviral activity against HSV2 strain 333 infected in african green monkey Vero cells assessed as inhibition of cytopathic effect after 48 hrs by plaque reduction assay, EC50=1.87 μM
WI-38 cell	Function assay		Anti viral activity against VZV(pplla strain) in WI-38 cell monolayers, ID50=4 μM
HEL cells	Function assay	4 days	Antiviral activity against HSV1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathogenicity after 4 days, EC50=0.2 μM
HEL cells	Function assay		Antiviral activity against HSV1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.14 μM
African green monkey Vero 76 cells	Cytotoxicity assay	48-96 h	Cytotoxicity against African green monkey Vero 76 cells assessed as cell viability after 48 to 96 hrs by crystal violet staining, CC50=13 μM
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 45 mg/mL (199.82 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.500mg/ml (11.10mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.500mg/ml (11.10mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	225.2	Formula	C₈H₁₁N₅O₃	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	59277-89-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Acycloguanosine, ACV, NSC 645011,BW 248U			Smiles	C1=NC2=C(N1COCCO)N=C(NC2=O)N

Mechanism of Action

Targets/IC₅₀/Ki	HSV
In vitro	In a plaque-reduction assay in Vero cells, the Acyclovir (Aciclovir) sensitivity of herpes simplex virus isolates is determined. IC50 Values are consistently 2-3 fold lower in B2 compared with the H strain of Vero cells. HSV Type 2 strains are 2-10-fold less sensitive than Type 1 strains.
In vivo	low-dose oral acyclovir (aciclovir) may be effective in the prevention of HSV infection during OKT3 treatment of seropositive patients. Continuation of this compound for two to four weeks following the conclusion of OKT3 therapy may prevent occurrence of delayed infections.
References	[1] https://pubmed.ncbi.nlm.nih.gov/3913459/ [2] https://pubmed.ncbi.nlm.nih.gov/3025149/ [3] https://pubmed.ncbi.nlm.nih.gov/2652575/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06228430	Not yet recruiting	Healthy Volunteer	International Bio service	February 12 2024	Phase 1
NCT05589688	Not yet recruiting	Obesity	University Hospital Toulouse	January 2024	Phase 1
NCT06058858	Not yet recruiting	Cytomegalovirus Infections\|Acute Leukemia\|B Cell Lymphoma	Assistance Publique - Hôpitaux de Paris	October 1 2023	--
NCT05468619	Recruiting	Herpes Simplex	National Institute of Allergy and Infectious Diseases (NIAID)	September 23 2022	Phase 1

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Acyclovir (Aciclovir) Antiviral chemical

Chemical Structure

Molecular Weight: 225.2