PF-562271

Catalog No.S2890

PF-562271 Chemical Structure

Molecular Weight(MW): 507.49

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.

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In DMSO USD 350 In stock
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USD 270 In stock
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4 Customer Reviews

  • (F,G) The migration and invasion ability of 5637 and T24 cells transfected with PF-562271 and WT-METTL13 was examined. The number of cells was quantified and is shown in a column graph.

    Sci Rep, 2016, 6:19261. PF-562271 purchased from Selleck.

    D-F. T47D cells plated as in A were treated with vehicle (−) or the FAK Y397 inhibitor, PF-562271 (+), for 1 h prior to ± PRL (4 nM) for 15 min (representative immunoblots).

    Oncotarget, 2016, 7(30):48093-48106. PF-562271 purchased from Selleck.

  • OVISE cells were incubated for 24 hr at the indicated concentrations of the FAK inhibitors. Immunoblots were performed to assess inhibition of auto-phosphorylation by the FAK inhibitors.

    PLoS One 2014 9(2), e88587. PF-562271 purchased from Selleck.

    VEGF and P450 enzyme production in response to FAK or Src inhibitors. MCF10A cells were cultured in LD collagen gels ?vehicle control (V), 10 uM PF-562271 FAK ATP inhibitor (PF) or Src inhibitor PP2 for 24 h. Cells were also lysed and examined for CYP1A1 or CYP4B1 by immunoblot (B and C) and densitometry (D and E). VEGF data are displayed as the mean normalized to the vehicle control, N = 6, 盨EM, *p < 0.003 vs control (A). P450 data are displayed as the mean relative to GAPDH and normalized to the control. N > 4, ±SEM, *p < 0.05 vs respective control (D and E).

    Environ Toxicol Pharmacol 2014 39(1), 114-124. PF-562271 purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 binds in the ATP-binding cleft of FAK, forming two of the three “canonical” H-bonds between the inhibitor and main-chain atoms in the kinase hinge region. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with IC50 of 5 nM. PF-562271 (3.3 μM) results in G1 arrest of PC3-M cells. [1] PF-562271 (1 nM) blocks bFGF-stimulated blood vessel angiogenesis as performed in chicken chorioallantoic membrane assays. PF-262271 potently blocks blood vessel sprouting without detectable changes in vascular leakage. [2] PF-562271 (250 nM) results in complete inhibition of collective A431 cell invasion into collagen gels. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 NUmzOVFtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPMNFZKSzVyPUCuNlc3PiEQvF2= NY\vW5FCW0GQR1XS
SW982 NWPLfJlTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTBwM{K4NkDPxE1? M1P2UXNCVkeHUh?=
KM12 NUfLR3hNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfyfmFKSzVyPUCuN|g2PTdizszN Ml3nV2FPT0WU
COLO-205 NVXwcoQ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXHJR|UxRTBwNEi2OVgh|ryP MVXTRW5ITVJ?
COLO-829 M2DzUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\PRWxKSzVyPUCuO|UyPzZizszN NESx[YdUSU6JRWK=
MG-63 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX23UnVJUUN3ME2wMlgxPjN5IN88US=> MWXTRW5ITVJ?
IGROV-1 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\xbmlEPTB;MD64NVA{QCEQvF2= NYr4R5FSW0GQR1XS
NCI-H650 NUm1O3NnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonITWM2OD1yLkizNVU1KM7:TR?= NGXvTnJUSU6JRWK=
RT-112 Mn;TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTBwOUi0OkDPxE1? MorrV2FPT0WU
BCPAP MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7NbnpKSzVyPUGuNFEzQDhizszN MlHoV2FPT0WU
ALL-PO NX3NRVBLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlOzTWM2OD1zLkCxOVg1KM7:TR?= M2DjeHNCVkeHUh?=
KYSE-270 NWP3OG82T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHL4NGZKSzVyPUGuNFQ4OTRizszN MYrTRW5ITVJ?
8305C M4Pxcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXmTWM2OD1zLkC5PVA1KM7:TR?= MlXXV2FPT0WU
NCI-H810 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTFwMUC3O|Yh|ryP MV3TRW5ITVJ?
CAL-33 NF60V4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEiyeZVKSzVyPUGuNVI6OzhizszN M2\PXnNCVkeHUh?=
AN3-CA M{XxO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LCcmlEPTB;MT6yNVg3PyEQvF2= NYK0WlBWW0GQR1XS
NKM-1 NEXHOGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn30TWM2OD1zLkK3OVA3KM7:TR?= M3qz[nNCVkeHUh?=
BPH-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnmT3FUUUN3ME2xMlI5PzZ4IN88US=> NUjGfWN1W0GQR1XS
MES-SA NXPGXnFTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnN[FRNUUN3ME2xMlMxPjh{IN88US=> NIrtO2tUSU6JRWK=
CAL-62 M2izcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTFwM{G5NFkh|ryP NWjMOWczW0GQR1XS
Detroit562 NGTRclFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PmVGlEPTB;MT6zOVIzOiEQvF2= NUHXfVExW0GQR1XS
KYSE-150 NVPkNWIyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDOPFdiUUN3ME2xMlM2OjN4IN88US=> NFHVToxUSU6JRWK=
SK-UT-1 M3PJUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\6TVhKSzVyPUGuOFQ3PDdizszN NYO2foZ7W0GQR1XS
HUTU-80 NFTMWHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjIXW1KSzVyPUGuOFQ5QDZizszN M1rYdXNCVkeHUh?=
SIG-M5 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTFwNEizPFch|ryP M3\HbnNCVkeHUh?=
AGS M4nzUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1z4eGlEPTB;MT61NlEzPCEQvF2= NFnoO5ZUSU6JRWK=
ST486 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoW1TWM2OD1zLkWzNlc5KM7:TR?= Ml\BV2FPT0WU
HSC-2 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX:3RppjUUN3ME2xMlU{QTVizszN NVfvcJhyW0GQR1XS
LCLC-103H NFXve2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIDJcIZKSzVyPUGuOVU5OThizszN MljyV2FPT0WU
BC-1 NVzxR2tJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17HO2lEPTB;MT62NVY3PCEQvF2= M3e5T3NCVkeHUh?=
CGTH-W-1 NUjHUo1LT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTFwNkG2O|kh|ryP MYTTRW5ITVJ?
MZ1-PC MnrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXS1WWMzUUN3ME2xMlYzOzF{IN88US=> MV7TRW5ITVJ?
SW1710 MkC0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;EWpBlUUN3ME2xMlYzPjJ6IN88US=> M{\US3NCVkeHUh?=
EW-13 NHnjOHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4S2RmlEPTB;MT62N|Q3PiEQvF2= Mk\oV2FPT0WU
TE-11 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnieIhSUUN3ME2xMlY{PTJ{IN88US=> MVHTRW5ITVJ?
BHY NHu1RoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;EV2lEPTB;MT62OVY3OyEQvF2= M1vvTXNCVkeHUh?=
ML-2 NFXZZmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXFU2pHUUN3ME2xMlY3QDB{IN88US=> MoP0V2FPT0WU
MEL-JUSO NUXpbI9jT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVTOO5BnUUN3ME2xMlczQDlzIN88US=> NGTaOZVUSU6JRWK=
U251 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlG5TWM2OD1zLke0NFMyKM7:TR?= NFr3dYNUSU6JRWK=
MHH-ES-1 NYXrZ41vT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfpPVFGUUN3ME2xMlc6PTZ6IN88US=> MljDV2FPT0WU
CAL-85-1 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTFwOEC0PUDPxE1? M4PBZ3NCVkeHUh?=
D-336MG MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPzZpNKSzVyPUGuPFU3OTRizszN M4H4fnNCVkeHUh?=
VA-ES-BJ MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIC4RYlKSzVyPUGuPVA{OTVizszN NEX3eHpUSU6JRWK=
MDA-MB-468 NVywOWE4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITxRXFKSzVyPUGuPVEzPzJizszN MUDTRW5ITVJ?
MEL-HO MojLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfRTWM2OD1zLkm3PVk{KM7:TR?= M170SnNCVkeHUh?=
SW954 M1PTc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13sZWlEPTB;Mj6wNFE2OSEQvF2= Mle3V2FPT0WU
LB831-BLC NFfFfWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zVNmlEPTB;Mj6wNFk5QCEQvF2= NXziZ4J4W0GQR1XS
EW-16 MonTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPiVGNKSzVyPUKuNFM6QDNizszN NVLOcGd4W0GQR1XS
NCI-H460 NGLyepVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTJwMES4N|kh|ryP MXHTRW5ITVJ?
CHP-212 M3TVVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrCTWM2OD1{LkC5OVM6KM7:TR?= M{\HV3NCVkeHUh?=
MHH-NB-11 M3PCVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XXcWlEPTB;Mj6xNlgyQCEQvF2= MkTMV2FPT0WU
DU-4475 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTJwMUS3OVkh|ryP M2W0SHNCVkeHUh?=
ABC-1 NFXQbXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{HHXmlEPTB;Mj6yOVE6PiEQvF2= MofpV2FPT0WU
LOUCY M16yfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXaRZpIUUN3ME2yMlI3OTJ6IN88US=> MYfTRW5ITVJ?
LC-2-ad M4\wfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzDe2t[UUN3ME2yMlI3QTZ4IN88US=> NIqyTmZUSU6JRWK=
ETK-1 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjhPHhKSzVyPUKuNlg1PiEQvF2= MX3TRW5ITVJ?
KYSE-180 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHjMSXBKSzVyPUKuNlg5PzRizszN MWLTRW5ITVJ?
HOP-92 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\aRWlEPTB;Mj6zNFY4OyEQvF2= NVG2b|NTW0GQR1XS
RCC10RGB NH\zOHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XWVWlEPTB;Mj6zNlUxPyEQvF2= M4LCTnNCVkeHUh?=
KASUMI-1 NHrONW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TQRWlEPTB;Mj6zNlg5KM7:TR?= M2XiSHNCVkeHUh?=
ACN NFjMb2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnTTmV5UUN3ME2yMlM1PzR3IN88US=> NU\VU2xsW0GQR1XS
BxPC-3 NYjLNoRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVm4UHdNUUN3ME2yMlM2PDN4IN88US=> MlzSV2FPT0WU
MONO-MAC-6 NYrSVVhMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUKzVGI3UUN3ME2yMlQyOjR6IN88US=> MnX0V2FPT0WU
CAL-39 Mlu1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTJwNEG2Nlgh|ryP MX\TRW5ITVJ?
CMK MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHKUHRKSzVyPUKuOFE4QTVizszN M2nGe3NCVkeHUh?=
MFE-296 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTJwNEe3PVIh|ryP M3PreHNCVkeHUh?=
DU-145 MoHVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTJwNEmxNVgh|ryP NYjVRmdzW0GQR1XS
MDA-MB-231 NYK0clJGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPRclFKSzVyPUKuOFk2PzJizszN MWnTRW5ITVJ?
EFO-27 NVvJcnJyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PiZ2lEPTB;Mj61NVg5QCEQvF2= MU\TRW5ITVJ?
SNU-387 MlrBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DGXmlEPTB;Mj61NlgzKM7:TR?= MYPTRW5ITVJ?
KYSE-410 MkOyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3S0VmlEPTB;Mj61OlUyPyEQvF2= MoTCV2FPT0WU
NCCIT NWXzcFFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTJwNUeyOEDPxE1? NF21XJZUSU6JRWK=
LXF-289 NUDuVW0yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRTJwNUiyO|Mh|ryP MUXTRW5ITVJ?
HMV-II MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\CcGlEPTB;Mj61PFk6PyEQvF2= NFWzc29USU6JRWK=
MOLT-4 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M370d2lEPTB;Mj62NFcyOiEQvF2= M{XiUHNCVkeHUh?=
KOSC-2 NX3SSZJVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHaTWM2OD1{Lk[zPFc6KM7:TR?= MoHyV2FPT0WU
TUR NGTWV4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;uTWM2OD1{Lk[1N|Y6KM7:TR?= NHjtdnJUSU6JRWK=
NCI-H2087 NFezRm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTJwN{G2OFYh|ryP NIe0VHVUSU6JRWK=
SCC-15 M2D3fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjkTWM2OD1{Lke1NlUzKM7:TR?= M4Kxd3NCVkeHUh?=
HSC-4 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXLYbJZVUUN3ME2yMlc5Ozh7IN88US=> NIHQOWJUSU6JRWK=
G-402 MmLjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIq3So5KSzVyPUKuPFE1PDdizszN M3\KbnNCVkeHUh?=
GP5d M{PuZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{i5fWlEPTB;Mj64N|gzPyEQvF2= MWnTRW5ITVJ?
IGR-1 M4\UVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrKPFl6UUN3ME2yMlg{QTh5IN88US=> M3nKUnNCVkeHUh?=
SK-PN-DW MnrBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTJwOE[yNFQh|ryP MWfTRW5ITVJ?
OPM-2 MmDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTJwOEm5O|gh|ryP Mo\wV2FPT0WU
5637 M1S3T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTJwOUK0N|kh|ryP M174TXNCVkeHUh?=
NCI-H2030 NUnOcnFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\le4RKSzVyPUKuPVgxOjlizszN NY\GToprW0GQR1XS
SK-LU-1 MnnSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jnT2lEPTB;Mj65PFM{PCEQvF2= NWi5UVJxW0GQR1XS
EW-18 NHT2VHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTNwMEWzNkDPxE1? M1;ZZ3NCVkeHUh?=
786-0 NEXBSZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTNwMEW3PFQh|ryP MWjTRW5ITVJ?
NCI-H1963 NF;DfVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\E[mlEPTB;Mz6wPFgzPSEQvF2= MWTTRW5ITVJ?
A2780 Mn7PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PDXmlEPTB;Mz6xNFM1OSEQvF2= NGK4RYRUSU6JRWK=
NCI-H526 MlzHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXTTWM2OD1|LkGzPVYyKM7:TR?= MnPzV2FPT0WU
BHT-101 NVrzZVA2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{iwS2lEPTB;Mz6xOVc6QCEQvF2= MV;TRW5ITVJ?
RPMI-7951 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWOzO2c5UUN3ME2zMlIxOTN5IN88US=> MlS3V2FPT0WU
HL-60 NVu3[21QT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LmNWlEPTB;Mz6yNVE1KM7:TR?= M1mwUHNCVkeHUh?=
CTV-1 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVexcXN1UUN3ME2zMlI2Ozd5IN88US=> NXTOZ3UxW0GQR1XS
IST-SL1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4Duc2lEPTB;Mz6yPVQ3PSEQvF2= MVTTRW5ITVJ?
G-401 MnnjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTNwMkm4NFgh|ryP NXnw[IhuW0GQR1XS
OVCAR-5 MkDZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrVXWdzUUN3ME2zMlM3OzV{IN88US=> MX\TRW5ITVJ?
EW-1 MmTFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTNwNEC1OFQh|ryP MWnTRW5ITVJ?
H4 NE[x[ZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHCS4Q1UUN3ME2zMlQ4PTZ7IN88US=> MX3TRW5ITVJ?
EM-2 NUi5RmNFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTNwNEi2O|Qh|ryP MmTVV2FPT0WU
RS4-11 NIfEO2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DTPWlEPTB;Mz60PFcyPSEQvF2= MmjhV2FPT0WU
NOMO-1 M4HNXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTNwNEmyNFQh|ryP MVjTRW5ITVJ?
HCT-116 NUDPUVNUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfpRlVqUUN3ME2zMlUxPzFzIN88US=> MojoV2FPT0WU
CAL-120 NIPze5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HCPGlEPTB;Mz61O|M2KM7:TR?= NWmxVXk6W0GQR1XS
CAL-51 M{nj[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorPTWM2OD1|LkW3OlEzKM7:TR?= MXPTRW5ITVJ?
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NCI-H2081 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH3ZOVJKSzVyPUOuOlUzQDhizszN M1nIfnNCVkeHUh?=
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NCI-H1437 Mk\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3RTWM2OD1zMj6xOFQ4KM7:TR?= MlnYV2FPT0WU
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NCI-H1792 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;kWmROUUN3ME2xNk4{QDVzIN88US=> MljMV2FPT0WU
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SW948 MmrJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlPETWM2OD1zMz6wNVMh|ryP NEfEV4dUSU6JRWK=
LB2241-RCC NHHsdVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTF|LkG0Olkh|ryP NVnTZVFyW0GQR1XS
CCF-STTG1 NHTVbo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHTbHhCUUN3ME2xN{41ODN7IN88US=> MWDTRW5ITVJ?
RPMI-8402 NHPYNmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnf6TWM2OD1zMz61NFMyKM7:TR?= MYXTRW5ITVJ?
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CAL-72 M4XKeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnQdldxUUN3ME2xOE4yQTB4IN88US=> NWe2enhCW0GQR1XS
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DB M2LVeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkWyTWM2OD1zNT62PVQyKM7:TR?= NV\FfIxrW0GQR1XS
Becker NWfDV49RT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHmOow2UUN3ME2xOU45QTJ4IN88US=> MXzTRW5ITVJ?
HT-144 NWnmXFdWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXINGJGUUN3ME2xOU46PDl5IN88US=> NFn3NXVUSU6JRWK=
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TE-8 M2fZWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjX[2hXUUN3ME2xPE4xPjZ7IN88US=> NIr5fJdUSU6JRWK=
UACC-257 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTF6LkKwOlIh|ryP M4mze3NCVkeHUh?=
NCI-H226 NIXib5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnLCTWM2OD1zOD64Nlk6KM7:TR?= M1rqeHNCVkeHUh?=
NCI-H748 M2O0Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTF6Lki1PFUh|ryP M1zvdHNCVkeHUh?=
NCI-H1299 NYDQc4xlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DoZWlEPTB;MUiuPFkyQCEQvF2= NHrnZlBUSU6JRWK=
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HCC1187 Mn;XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTF7LkG3PVYh|ryP NHXs[ppUSU6JRWK=
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SK-MEL-2 NEnkb4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4G0WmlEPTB;MUmuNlE2PSEQvF2= NFz1R|RUSU6JRWK=
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LB996-RCC Mn3pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TsUGlEPTB;NEKuPFAxQCEQvF2= NXn5TGVYW0GQR1XS
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MRK-nu-1 NXzOXGY6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTR2Lke0NVQh|ryP MWXTRW5ITVJ?
EoL-1-cell NU\HbFBkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXSxTFk2UUN3ME20OE46OTN4IN88US=> NVjEd3B7W0GQR1XS
NCI-H2342 NYfKNFVyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjjTGk3UUN3ME20OU4yOTF5IN88US=> NI\IS3hUSU6JRWK=
NCI-H1703 MofyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHGyOYlKSzVyPUS2MlA2QTJizszN MoToV2FPT0WU
NCI-H187 NULqcYhWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIGyR2lKSzVyPUS2MlE5PjlizszN NVr0SYVkW0GQR1XS
CP66-MEL NV62dodRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVSxZ494UUN3ME20Ok4zPzh7IN88US=> MoXnV2FPT0WU
HCC1569 NVPwbIhoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\QdoU6UUN3ME20Ok42QTF5IN88US=> NWXEOHlwW0GQR1XS
BL-70 NVjHSVVFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTR4Lke3N|ch|ryP NEntXYtUSU6JRWK=
Saos-2 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXP0VGRzUUN3ME20O{4{OTJ|IN88US=> NFuzN4ZUSU6JRWK=
LC-1F NEfXbHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXFUmZKSzVyPUS3MlYyODVizszN NVriVZRlW0GQR1XS
KY821 M360Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PGc2lEPTB;NEeuOlQ{PCEQvF2= M2HobXNCVkeHUh?=
PSN1 NIjZNnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX3WUGJ5UUN3ME20PE4yPDBzIN88US=> NHTBPXRUSU6JRWK=
RT4 MmDCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTR6LkizO|ch|ryP MnvkV2FPT0WU
LS-1034 MoC4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\z[|NKSzVyPUS4Mlg6PTRizszN NHLxVY5USU6JRWK=
NCI-H1395 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTR7LkC0PVIh|ryP NIj3SJpUSU6JRWK=
EKVX M1XK[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1XMbWlEPTB;NEmuOlc{OSEQvF2= NUnCOIc2W0GQR1XS
IST-MES1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnjTWM2OD12OT64PVQ1KM7:TR?= NV:0W|lSW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo PF-562271 (< 33 mg/kg p.o.) inhibits FAK phosphorylation in tumors in a dose- and time-dependent manner in U87MG-bearing mice. PF-562271 (50 mg/kg p.o. bid) results in 86% tumor growth inhibition in BxPc3 xenografts mice and 45% tumor growth inhibition in PC3-M xenografts mice. PF-562271 (25 mg/kg, bid) results in 2-fold greater apoptosis in treated tumors in mice bearing H125 lung xenografts. [1] PF-562271 (33 mg/kg, p.o.) inhibits extensive movement of the tumor cells over 24 hours in mice. PF-562271 (33 mg/kg, p.o.) results in altered E-cadherin dynamics in mice, which correlates with reduced E-cadherin–dependent collective cell movement. [3] PF-562271 (25 mg/kg, p.o. bid) results in 62% tumor growth inhibition in PC3M-luc-C6 subcutaneuous local implant xenograft mouse model. [4] PF-562,271 (5 mg/kg, oral) results in significant and similar increases in osteocalcin and cancellous bone parameters and a decrease in tumor growth and signs of bone healing in rats implanted with MDA-MB-231 cells in the tibia. [5]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Athymic female mice bearing BxPc3 or PC3-M xenografts.
  • Formulation: 5% Gelucire
  • Dosages: 50 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (197.04 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.49
Formula

C21H20F3N7O3S

CAS No. 717907-75-0
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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FAK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID