PF-562271

Catalog No.S2890

PF-562271 Chemical Structure

Molecular Weight(MW): 507.49

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.

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In DMSO USD 350 In stock
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4 Customer Reviews

  • (F,G) The migration and invasion ability of 5637 and T24 cells transfected with PF-562271 and WT-METTL13 was examined. The number of cells was quantified and is shown in a column graph.

    Sci Rep, 2016, 6:19261. PF-562271 purchased from Selleck.

    D-F. T47D cells plated as in A were treated with vehicle (−) or the FAK Y397 inhibitor, PF-562271 (+), for 1 h prior to ± PRL (4 nM) for 15 min (representative immunoblots).

    Oncotarget, 2016, 7(30):48093-48106. PF-562271 purchased from Selleck.

  • OVISE cells were incubated for 24 hr at the indicated concentrations of the FAK inhibitors. Immunoblots were performed to assess inhibition of auto-phosphorylation by the FAK inhibitors.

    PLoS One 2014 9(2), e88587. PF-562271 purchased from Selleck.

    VEGF and P450 enzyme production in response to FAK or Src inhibitors. MCF10A cells were cultured in LD collagen gels ?vehicle control (V), 10 uM PF-562271 FAK ATP inhibitor (PF) or Src inhibitor PP2 for 24 h. Cells were also lysed and examined for CYP1A1 or CYP4B1 by immunoblot (B and C) and densitometry (D and E). VEGF data are displayed as the mean normalized to the vehicle control, N = 6, 盨EM, *p < 0.003 vs control (A). P450 data are displayed as the mean relative to GAPDH and normalized to the control. N > 4, ±SEM, *p < 0.05 vs respective control (D and E).

    Environ Toxicol Pharmacol 2014 39(1), 114-124. PF-562271 purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 binds in the ATP-binding cleft of FAK, forming two of the three “canonical” H-bonds between the inhibitor and main-chain atoms in the kinase hinge region. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with IC50 of 5 nM. PF-562271 (3.3 μM) results in G1 arrest of PC3-M cells. [1] PF-562271 (1 nM) blocks bFGF-stimulated blood vessel angiogenesis as performed in chicken chorioallantoic membrane assays. PF-262271 potently blocks blood vessel sprouting without detectable changes in vascular leakage. [2] PF-562271 (250 nM) results in complete inhibition of collective A431 cell invasion into collagen gels. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 NWLYTm9IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn[4TWM2OD1yLkK3OlYh|ryP MoHEV2FPT0WU
SW982 M3PEUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\GTWM2OD1yLkOyPFIh|ryP NXjhRppLW0GQR1XS
KM12 NWraRm86T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{C2[GlEPTB;MD6zPFU2PyEQvF2= MVzTRW5ITVJ?
COLO-205 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3CUZZKSzVyPUCuOFg3PThizszN NVmwNIltW0GQR1XS
COLO-829 NYP2[IhRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PmT2lEPTB;MD63OVE4PiEQvF2= M4TBZnNCVkeHUh?=
MG-63 Mkm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmnGTWM2OD1yLkiwOlM4KM7:TR?= M1\Ve3NCVkeHUh?=
IGROV-1 Mn[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHMR45KSzVyPUCuPFExOzhizszN NF\vdGdUSU6JRWK=
NCI-H650 NFLWV|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4W2TGlEPTB;MD64N|E2PCEQvF2= NIK2VoVUSU6JRWK=
RT-112 NHX6O3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTBwOUi0OkDPxE1? MnnpV2FPT0WU
BCPAP NUDGfpNiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4frXmlEPTB;MT6wNVI5QCEQvF2= M1LIUnNCVkeHUh?=
ALL-PO NF;zdVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHUZXlTUUN3ME2xMlAyPTh2IN88US=> M3jO[3NCVkeHUh?=
KYSE-270 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTFwMES3NVQh|ryP MY\TRW5ITVJ?
8305C NI\nUFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGn1Z3pKSzVyPUGuNFk6ODRizszN NI\ZWWlUSU6JRWK=
NCI-H810 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fNe2lEPTB;MT6xNFc4PiEQvF2= M{HVNXNCVkeHUh?=
CAL-33 Mn7nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTFwMUK5N|gh|ryP M1;3SXNCVkeHUh?=
AN3-CA M3T0Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\TTWM2OD1zLkKxPFY4KM7:TR?= M1r0bHNCVkeHUh?=
NKM-1 Mki4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\OfmlEPTB;MT6yO|UxPiEQvF2= MnfuV2FPT0WU
BPH-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M134SmlEPTB;MT6yPFc3PiEQvF2= MUfTRW5ITVJ?
MES-SA M4[xWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrtfFNKSzVyPUGuN|A3QDJizszN NH;JXFBUSU6JRWK=
CAL-62 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDMTWM2OD1zLkOxPVA6KM7:TR?= NY\m[oFkW0GQR1XS
Detroit562 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHsephKSzVyPUGuN|UzOjJizszN MV7TRW5ITVJ?
KYSE-150 MkjxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHiSVVKSzVyPUGuN|UzOzZizszN MkfMV2FPT0WU
SK-UT-1 NXvidpdnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7sWXFjUUN3ME2xMlQ1PjR5IN88US=> MkfuV2FPT0WU
HUTU-80 NUTTdZlFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTKTWM2OD1zLkS0PFg3KM7:TR?= MVzTRW5ITVJ?
SIG-M5 MmHnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYKxV4lSUUN3ME2xMlQ5Ozh5IN88US=> NXPyeIJ6W0GQR1XS
AGS NGDUO4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\pTWM2OD1zLkWyNVI1KM7:TR?= M3nDSHNCVkeHUh?=
ST486 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmC5TWM2OD1zLkWzNlc5KM7:TR?= MYjTRW5ITVJ?
HSC-2 NEXE[GdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnQZVlKSzVyPUGuOVM6PSEQvF2= NHPtSmFUSU6JRWK=
LCLC-103H NELofnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDXTIF7UUN3ME2xMlU2QDF6IN88US=> MVvTRW5ITVJ?
BC-1 NYrzSZU{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlr6TWM2OD1zLk[xOlY1KM7:TR?= NEfLUXRUSU6JRWK=
CGTH-W-1 MlHoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGi3NGVKSzVyPUGuOlE3PzlizszN NHHoOm5USU6JRWK=
MZ1-PC Mn\OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTFwNkKzNVIh|ryP NYe1eJlSW0GQR1XS
SW1710 MnvJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzGfotKSzVyPUGuOlI3OjhizszN MYLTRW5ITVJ?
EW-13 NFTvd|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;sTWM2OD1zLk[zOFY3KM7:TR?= NFHiW49USU6JRWK=
TE-11 M1HlXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4Ti[GlEPTB;MT62N|UzOiEQvF2= NVHZb2FoW0GQR1XS
BHY MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\4TGlEPTB;MT62OVY3OyEQvF2= MV3TRW5ITVJ?
ML-2 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPWTWM2OD1zLk[2PFAzKM7:TR?= NWTQcnhZW0GQR1XS
MEL-JUSO MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTFwN{K4PVEh|ryP NWTBR5VbW0GQR1XS
U251 M2DU[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTFwN{SwN|Eh|ryP M{DWTHNCVkeHUh?=
MHH-ES-1 NU\QNZpKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYTMb|NvUUN3ME2xMlc6PTZ6IN88US=> NWPwbZJkW0GQR1XS
CAL-85-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{D1dWlEPTB;MT64NFQ6KM7:TR?= MnvGV2FPT0WU
D-336MG M{e3Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;MTnhRUUN3ME2xMlg2PjF2IN88US=> MnzHV2FPT0WU
VA-ES-BJ MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnTOTWM2OD1zLkmwN|E2KM7:TR?= NH;0ZYNUSU6JRWK=
MDA-MB-468 M4LBbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHNTWM2OD1zLkmxNlczKM7:TR?= NEm5N3lUSU6JRWK=
MEL-HO NIjZOmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvXPJJKSzVyPUGuPVc6QTNizszN M3TqcXNCVkeHUh?=
SW954 M3vDcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGf6S4xKSzVyPUKuNFAyPTFizszN NHfw[mhUSU6JRWK=
LB831-BLC NHTwNWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rn[2lEPTB;Mj6wNFk5QCEQvF2= M3rucnNCVkeHUh?=
EW-16 NXj5SXZtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTJwMEO5PFMh|ryP MVzTRW5ITVJ?
NCI-H460 NGr4TVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XrN2lEPTB;Mj6wOFg{QSEQvF2= NWD4SFdEW0GQR1XS
CHP-212 NXLRSG1XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTJwMEm1N|kh|ryP MWnTRW5ITVJ?
MHH-NB-11 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTJwMUK4NVgh|ryP M3rHNXNCVkeHUh?=
DU-4475 MoPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3KTWM2OD1{LkG0O|U6KM7:TR?= NHflfYZUSU6JRWK=
ABC-1 M1G4OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTJwMkWxPVYh|ryP M3ruSnNCVkeHUh?=
LOUCY M3jYV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nOeGlEPTB;Mj6yOlEzQCEQvF2= M{LIN3NCVkeHUh?=
LC-2-ad NWr2SJRRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVq2VnlEUUN3ME2yMlI3QTZ4IN88US=> M1noUXNCVkeHUh?=
ETK-1 NUHRUYg5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHLnPYFKSzVyPUKuNlg1PiEQvF2= MVTTRW5ITVJ?
KYSE-180 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXMcFdKSzVyPUKuNlg5PzRizszN MoX3V2FPT0WU
HOP-92 NHfqToxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTYdocyUUN3ME2yMlMxPjd|IN88US=> MmH3V2FPT0WU
RCC10RGB MnT3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULZOnh{UUN3ME2yMlMzPTB5IN88US=> Mn3tV2FPT0WU
KASUMI-1 M2nLTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXFTWM2OD1{LkOyPFgh|ryP NHPxOnlUSU6JRWK=
ACN MoTJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTNb3hKSzVyPUKuN|Q4PDVizszN MljuV2FPT0WU
BxPC-3 NEnmNYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DFWmlEPTB;Mj6zOVQ{PiEQvF2= NGLMS29USU6JRWK=
MONO-MAC-6 MojNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWO0OodqUUN3ME2yMlQyOjR6IN88US=> MkfDV2FPT0WU
CAL-39 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHnTWM2OD1{LkSxOlI5KM7:TR?= NFXHR5ZUSU6JRWK=
CMK MlHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEW4R5NKSzVyPUKuOFE4QTVizszN MlnJV2FPT0WU
MFE-296 MkiyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoT6TWM2OD1{LkS3O|kzKM7:TR?= M2fMTnNCVkeHUh?=
DU-145 NXn0T|ZXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mkf4TWM2OD1{LkS5NVE5KM7:TR?= NWHJR|BXW0GQR1XS
MDA-MB-231 M3\ESmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFniNW1KSzVyPUKuOFk2PzJizszN MonnV2FPT0WU
EFO-27 NEf3cZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTHblJKSzVyPUKuOVE5QDhizszN NWXDPJJVW0GQR1XS
SNU-387 NGe0bJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PGNGlEPTB;Mj61NlgzKM7:TR?= M3zzSHNCVkeHUh?=
KYSE-410 NHzJOnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXmydlVjUUN3ME2yMlU3PTF5IN88US=> NH;DcI9USU6JRWK=
NCCIT NW[0N2hGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7xUXNqUUN3ME2yMlU4OjRizszN M3Xld3NCVkeHUh?=
LXF-289 M{jVR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjjTWM2OD1{LkW4Nlc{KM7:TR?= MlXkV2FPT0WU
HMV-II NX[zbWxPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\z[2lEPTB;Mj61PFk6PyEQvF2= NFXNUlBUSU6JRWK=
MOLT-4 NFTWc|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfFb2YyUUN3ME2yMlYxPzF{IN88US=> MYDTRW5ITVJ?
KOSC-2 M4K2emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzFTWM2OD1{Lk[zPFc6KM7:TR?= MXHTRW5ITVJ?
TUR NFvWcHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfONmpKUUN3ME2yMlY2OzZ7IN88US=> MkXaV2FPT0WU
NCI-H2087 Mni4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DXcWlEPTB;Mj63NVY1PiEQvF2= MnizV2FPT0WU
SCC-15 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPTTWM2OD1{Lke1NlUzKM7:TR?= NInpZ5JUSU6JRWK=
HSC-4 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1L4[GlEPTB;Mj63PFM5QSEQvF2= MWLTRW5ITVJ?
G-402 Ml7rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITaVVdKSzVyPUKuPFE1PDdizszN NGPnPZRUSU6JRWK=
GP5d NV7vSHJWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXYSFVDUUN3ME2yMlg{QDJ5IN88US=> M4DZ[HNCVkeHUh?=
IGR-1 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnPKTWM2OD1{LkizPVg4KM7:TR?= NFjRV|NUSU6JRWK=
SK-PN-DW NV2wOWw3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIn3fHhKSzVyPUKuPFYzODRizszN M2D5NHNCVkeHUh?=
OPM-2 NEe3VWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPqNVM5UUN3ME2yMlg6QTd6IN88US=> MX;TRW5ITVJ?
5637 NVrDOlZXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLSVpZxUUN3ME2yMlkzPDN7IN88US=> MUDTRW5ITVJ?
NCI-H2030 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjtTWM2OD1{Lkm4NFI6KM7:TR?= MWTTRW5ITVJ?
SK-LU-1 MlXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XM[GlEPTB;Mj65PFM{PCEQvF2= M2LqTnNCVkeHUh?=
EW-18 MnznS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fEbmlEPTB;Mz6wOVMzKM7:TR?= NH7DTXpUSU6JRWK=
786-0 MmnGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;D[W9NUUN3ME2zMlA2Pzh2IN88US=> NFXQTXNUSU6JRWK=
NCI-H1963 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2TFZWlEPTB;Mz6wPFgzPSEQvF2= Mlj2V2FPT0WU
A2780 M3;ZWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFy1VYpKSzVyPUOuNVA{PDFizszN NV3jZXJmW0GQR1XS
NCI-H526 Mo[3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrSfpRoUUN3ME2zMlE{QTZzIN88US=> MneyV2FPT0WU
BHT-101 NVnZU5V7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTNwMUW3PVgh|ryP NYrwd3NEW0GQR1XS
RPMI-7951 Ml\hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrYfGg6UUN3ME2zMlIxOTN5IN88US=> M1fOb3NCVkeHUh?=
HL-60 MoDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4G4fWlEPTB;Mz6yNVE1KM7:TR?= M4rWcnNCVkeHUh?=
CTV-1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnSbVJkUUN3ME2zMlI2Ozd5IN88US=> Mm\IV2FPT0WU
IST-SL1 NG\VcVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\1TWM2OD1|LkK5OFY2KM7:TR?= MWfTRW5ITVJ?
G-401 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTNwMkm4NFgh|ryP M2P4NHNCVkeHUh?=
OVCAR-5 NIDJNYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrTeldKSzVyPUOuN|Y{PTJizszN NXXDfG5PW0GQR1XS
EW-1 MmKwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2[1b2lEPTB;Mz60NFU1PCEQvF2= NFHMTIRUSU6JRWK=
H4 MljBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlz2TWM2OD1|LkS3OVY6KM7:TR?= NG\Lc3NUSU6JRWK=
EM-2 NIPh[VFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTNwNEi2O|Qh|ryP NIf2XGdUSU6JRWK=
RS4-11 Moj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTNwNEi3NVUh|ryP NI\2VodUSU6JRWK=
NOMO-1 NF\UXWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfIOYRKSzVyPUOuOFkzODRizszN MV3TRW5ITVJ?
HCT-116 M1G2Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnneIY3UUN3ME2zMlUxPzFzIN88US=> NUX0bnFZW0GQR1XS
CAL-120 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTNwNUezOUDPxE1? NXHGdHFQW0GQR1XS
CAL-51 M1z3Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHSXJFKSzVyPUOuOVc3OTJizszN NEG4[mFUSU6JRWK=
RVH-421 NHvLeZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PRV2lEPTB;Mz62NFMyOSEQvF2= M{KxRnNCVkeHUh?=
NCI-H2081 MmPsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInBcnBKSzVyPUOuOlUzQDhizszN NV:0T4NoW0GQR1XS
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MRK-nu-1 MnfRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnL3TWM2OD12ND63OFE1KM7:TR?= MlzPV2FPT0WU
EoL-1-cell MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLsTncxUUN3ME20OE46OTN4IN88US=> NYDtdm5WW0GQR1XS
NCI-H2342 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTlTWM2OD12NT6xNVE4KM7:TR?= M2n6XnNCVkeHUh?=
NCI-H1703 MnruS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTR4LkC1PVIh|ryP MULTRW5ITVJ?
NCI-H187 M2HrZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU\JR|UxRTR4LkG4Olkh|ryP NHLUcW1USU6JRWK=
CP66-MEL M4TqPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLGTWM2OD12Nj6yO|g6KM7:TR?= NWT1cWd[W0GQR1XS
HCC1569 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnCTWM2OD12Nj61PVE4KM7:TR?= NGricnJUSU6JRWK=
BL-70 NInoU5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHCU5dqUUN3ME20Ok44PzN5IN88US=> MlSxV2FPT0WU
Saos-2 NEXaNmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTR5LkOxNlMh|ryP MXLTRW5ITVJ?
LC-1F MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2SxemlEPTB;NEeuOlExPSEQvF2= MoDBV2FPT0WU
KY821 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LlcmlEPTB;NEeuOlQ{PCEQvF2= NXr0NGhnW0GQR1XS
PSN1 NEj4ZpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Moq4TWM2OD12OD6xOFAyKM7:TR?= NUfmSW53W0GQR1XS
RT4 MlfVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\4VplkUUN3ME20PE45Ozd5IN88US=> MWfTRW5ITVJ?
LS-1034 NEXwOFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTR6Lki5OVQh|ryP NYfX[ZROW0GQR1XS
NCI-H1395 NGTIdWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTR7LkC0PVIh|ryP M3XmeXNCVkeHUh?=
EKVX NETyOmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3fxXGlEPTB;NEmuOlc{OSEQvF2= MX\TRW5ITVJ?
IST-MES1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWe2fnFnUUN3ME20PU45QTR2IN88US=> MYfTRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo PF-562271 (< 33 mg/kg p.o.) inhibits FAK phosphorylation in tumors in a dose- and time-dependent manner in U87MG-bearing mice. PF-562271 (50 mg/kg p.o. bid) results in 86% tumor growth inhibition in BxPc3 xenografts mice and 45% tumor growth inhibition in PC3-M xenografts mice. PF-562271 (25 mg/kg, bid) results in 2-fold greater apoptosis in treated tumors in mice bearing H125 lung xenografts. [1] PF-562271 (33 mg/kg, p.o.) inhibits extensive movement of the tumor cells over 24 hours in mice. PF-562271 (33 mg/kg, p.o.) results in altered E-cadherin dynamics in mice, which correlates with reduced E-cadherin–dependent collective cell movement. [3] PF-562271 (25 mg/kg, p.o. bid) results in 62% tumor growth inhibition in PC3M-luc-C6 subcutaneuous local implant xenograft mouse model. [4] PF-562,271 (5 mg/kg, oral) results in significant and similar increases in osteocalcin and cancellous bone parameters and a decrease in tumor growth and signs of bone healing in rats implanted with MDA-MB-231 cells in the tibia. [5]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Athymic female mice bearing BxPc3 or PC3-M xenografts.
  • Formulation: 5% Gelucire
  • Dosages: 50 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (197.04 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.49
Formula

C21H20F3N7O3S

CAS No. 717907-75-0
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

FAK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID