PF-562271

Catalog No.S2890

PF-562271 Chemical Structure

Molecular Weight(MW): 507.49

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.

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In DMSO USD 350 In stock
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USD 270 In stock
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4 Customer Reviews

  • (F,G) The migration and invasion ability of 5637 and T24 cells transfected with PF-562271 and WT-METTL13 was examined. The number of cells was quantified and is shown in a column graph.

    Sci Rep, 2016, 6:19261. PF-562271 purchased from Selleck.

    D-F. T47D cells plated as in A were treated with vehicle (−) or the FAK Y397 inhibitor, PF-562271 (+), for 1 h prior to ± PRL (4 nM) for 15 min (representative immunoblots).

    Oncotarget, 2016, 7(30):48093-48106. PF-562271 purchased from Selleck.

  • OVISE cells were incubated for 24 hr at the indicated concentrations of the FAK inhibitors. Immunoblots were performed to assess inhibition of auto-phosphorylation by the FAK inhibitors.

    PLoS One 2014 9(2), e88587. PF-562271 purchased from Selleck.

    VEGF and P450 enzyme production in response to FAK or Src inhibitors. MCF10A cells were cultured in LD collagen gels ?vehicle control (V), 10 uM PF-562271 FAK ATP inhibitor (PF) or Src inhibitor PP2 for 24 h. Cells were also lysed and examined for CYP1A1 or CYP4B1 by immunoblot (B and C) and densitometry (D and E). VEGF data are displayed as the mean normalized to the vehicle control, N = 6, 盨EM, *p < 0.003 vs control (A). P450 data are displayed as the mean relative to GAPDH and normalized to the control. N > 4, ±SEM, *p < 0.05 vs respective control (D and E).

    Environ Toxicol Pharmacol 2014 39(1), 114-124. PF-562271 purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 binds in the ATP-binding cleft of FAK, forming two of the three “canonical” H-bonds between the inhibitor and main-chain atoms in the kinase hinge region. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with IC50 of 5 nM. PF-562271 (3.3 μM) results in G1 arrest of PC3-M cells. [1] PF-562271 (1 nM) blocks bFGF-stimulated blood vessel angiogenesis as performed in chicken chorioallantoic membrane assays. PF-262271 potently blocks blood vessel sprouting without detectable changes in vascular leakage. [2] PF-562271 (250 nM) results in complete inhibition of collective A431 cell invasion into collagen gels. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 NWXKVFE3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTBwMke2OkDPxE1? M4\MbXNCVkeHUh?=
SW982 NHnOZ5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;ZTWM2OD1yLkOyPFIh|ryP NW\LTmFvW0GQR1XS
KM12 M2C5c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTBwM{i1OVch|ryP Ml:4V2FPT0WU
COLO-205 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4TMRmlEPTB;MD60PFY2QCEQvF2= MXjTRW5ITVJ?
COLO-829 NUTt[3ZCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXq1VnNmUUN3ME2wMlc2OTd4IN88US=> MWXTRW5ITVJ?
MG-63 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLrRYt2UUN3ME2wMlgxPjN5IN88US=> NITXOWhUSU6JRWK=
IGROV-1 NVTNeYhNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnXd2xKSzVyPUCuPFExOzhizszN MmXNV2FPT0WU
NCI-H650 MnjHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTBwOEOxOVQh|ryP MkPjV2FPT0WU
RT-112 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXXTWM2OD1yLkm4OFYh|ryP MlPTV2FPT0WU
BCPAP NHL4d4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDETWM2OD1zLkCxNlg5KM7:TR?= MY\TRW5ITVJ?
ALL-PO NWj6RXp1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTFwMEG1PFQh|ryP MYnTRW5ITVJ?
KYSE-270 MkjCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDPNG5KSzVyPUGuNFQ4OTRizszN MnKwV2FPT0WU
8305C M1v0UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTFwMEm5NFQh|ryP MkHoV2FPT0WU
NCI-H810 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGqzOYZKSzVyPUGuNVA4PzZizszN NHnj[IRUSU6JRWK=
CAL-33 NYjWWWZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWmycpRnUUN3ME2xMlEzQTN6IN88US=> MlH2V2FPT0WU
AN3-CA M1PyV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTFwMkG4Olch|ryP NIC3V41USU6JRWK=
NKM-1 NXO0SYlNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEDSUJBKSzVyPUGuNlc2ODZizszN MXfTRW5ITVJ?
BPH-1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTFwMki3OlYh|ryP MlK3V2FPT0WU
MES-SA NVrlc2E6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DZUWlEPTB;MT6zNFY5OiEQvF2= MYrTRW5ITVJ?
CAL-62 NFHPTYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTFwM{G5NFkh|ryP NIXiVnRUSU6JRWK=
Detroit562 M4jEWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfwT|RKSzVyPUGuN|UzOjJizszN MXHTRW5ITVJ?
KYSE-150 MoTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEj3ZW9KSzVyPUGuN|UzOzZizszN M4fsNXNCVkeHUh?=
SK-UT-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDVcJdLUUN3ME2xMlQ1PjR5IN88US=> NHLWZopUSU6JRWK=
HUTU-80 M3zKe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TDbWlEPTB;MT60OFg5PiEQvF2= NWD5N4dDW0GQR1XS
SIG-M5 M1vYdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jKbmlEPTB;MT60PFM5PyEQvF2= NETsenlUSU6JRWK=
AGS MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LKVmlEPTB;MT61NlEzPCEQvF2= NIfMTpRUSU6JRWK=
ST486 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnBTWM2OD1zLkWzNlc5KM7:TR?= Mlr5V2FPT0WU
HSC-2 MkjJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XwWWlEPTB;MT61N|k2KM7:TR?= MoH4V2FPT0WU
LCLC-103H MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTFwNUW4NVgh|ryP MYnTRW5ITVJ?
BC-1 NWL4ZnpyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnXJTWM2OD1zLk[xOlY1KM7:TR?= NXi3Vmw6W0GQR1XS
CGTH-W-1 M{exOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvQO|VFUUN3ME2xMlYyPjd7IN88US=> NXnUOYh3W0GQR1XS
MZ1-PC M3j0eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIrzb5hKSzVyPUGuOlI{OTJizszN NWPDZ2syW0GQR1XS
SW1710 MlHlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4mydWlEPTB;MT62NlYzQCEQvF2= NWnRbGlpW0GQR1XS
EW-13 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{Kz[GlEPTB;MT62N|Q3PiEQvF2= MXvTRW5ITVJ?
TE-11 NVTnSVBnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NESxfllKSzVyPUGuOlM2OjJizszN MVPTRW5ITVJ?
BHY NFfGb29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4LW[mlEPTB;MT62OVY3OyEQvF2= MXrTRW5ITVJ?
ML-2 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIOyVZJKSzVyPUGuOlY5ODJizszN NFfYRWFUSU6JRWK=
MEL-JUSO NUDnflZ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnCTWM2OD1zLkeyPFkyKM7:TR?= MmDrV2FPT0WU
U251 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLNbIw2UUN3ME2xMlc1ODNzIN88US=> MoHiV2FPT0WU
MHH-ES-1 M1LYOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzufHV2UUN3ME2xMlc6PTZ6IN88US=> MlnhV2FPT0WU
CAL-85-1 M1jiRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUSzSY9sUUN3ME2xMlgxPDlizszN Ml62V2FPT0WU
D-336MG Ml3vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTmTWM2OD1zLki1OlE1KM7:TR?= MXnTRW5ITVJ?
VA-ES-BJ MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LGWWlEPTB;MT65NFMyPSEQvF2= Mm\vV2FPT0WU
MDA-MB-468 M4rpU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYL3NFRDUUN3ME2xMlkyOjd{IN88US=> NILoS49USU6JRWK=
MEL-HO M13BUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTFwOUe5PVMh|ryP NHP4XXJUSU6JRWK=
SW954 M{HKXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDKV4NFUUN3ME2yMlAxOTVzIN88US=> NHnZXJRUSU6JRWK=
LB831-BLC NUSxVG9tT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTmPHRKSzVyPUKuNFA6QDhizszN MlKxV2FPT0WU
EW-16 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGXxRXdKSzVyPUKuNFM6QDNizszN M{PIdnNCVkeHUh?=
NCI-H460 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnITWM2OD1{LkC0PFM6KM7:TR?= M{PlenNCVkeHUh?=
CHP-212 M4rNTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHBTWM2OD1{LkC5OVM6KM7:TR?= NGTlbZZUSU6JRWK=
MHH-NB-11 MkXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLjS|hHUUN3ME2yMlEzQDF6IN88US=> M3jF[nNCVkeHUh?=
DU-4475 NIDGOoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXsTWM2OD1{LkG0O|U6KM7:TR?= Mo[yV2FPT0WU
ABC-1 NYfwR3ZZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3fpUmlEPTB;Mj6yOVE6PiEQvF2= NWPDW4pJW0GQR1XS
LOUCY NHfO[HFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\qfmlEPTB;Mj6yOlEzQCEQvF2= M3;URXNCVkeHUh?=
LC-2-ad NGP6fYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\qfmlEPTB;Mj6yOlk3PiEQvF2= NWC2VodqW0GQR1XS
ETK-1 NGXTPHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTJwMki0OkDPxE1? MlHJV2FPT0WU
KYSE-180 MnPaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjEUIRKSzVyPUKuNlg5PzRizszN NH:wZpBUSU6JRWK=
HOP-92 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTJwM{C2O|Mh|ryP MoS2V2FPT0WU
RCC10RGB NY[xblR[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTJwM{K1NFch|ryP MXLTRW5ITVJ?
KASUMI-1 MnvtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTJwM{K4PEDPxE1? MUPTRW5ITVJ?
ACN NXS0fnBiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTJwM{S3OFUh|ryP M{XB[3NCVkeHUh?=
BxPC-3 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTJwM{W0N|Yh|ryP NH;iSYpUSU6JRWK=
MONO-MAC-6 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DmNmlEPTB;Mj60NVI1QCEQvF2= NIj2ZYhUSU6JRWK=
CAL-39 M2Pyd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXqR3h3UUN3ME2yMlQyPjJ6IN88US=> NYPHdXZ{W0GQR1XS
CMK NFTyRoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTJwNEG3PVUh|ryP MoH3V2FPT0WU
MFE-296 Mkm4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIPtPWtKSzVyPUKuOFc4QTJizszN NGn0cI5USU6JRWK=
DU-145 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1v2fmlEPTB;Mj60PVEyQCEQvF2= NVPnUXEyW0GQR1XS
MDA-MB-231 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HnUWlEPTB;Mj60PVU4OiEQvF2= NEPIcZFUSU6JRWK=
EFO-27 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTJwNUG4PFgh|ryP MlTNV2FPT0WU
SNU-387 MmHHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\nVpVKSzVyPUKuOVI5OiEQvF2= NHvKS|VUSU6JRWK=
KYSE-410 NW\CNJhIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fSUmlEPTB;Mj61OlUyPyEQvF2= NHLXWW5USU6JRWK=
NCCIT M3;rPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;lZoRKSzVyPUKuOVczPCEQvF2= Mn3GV2FPT0WU
LXF-289 NFvEN4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnWzTWM2OD1{LkW4Nlc{KM7:TR?= Mn7iV2FPT0WU
HMV-II M4f5ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPQOotKSzVyPUKuOVg6QTdizszN NGfoeHZUSU6JRWK=
MOLT-4 M3PDOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjlcnZrUUN3ME2yMlYxPzF{IN88US=> NHH5ZZRUSU6JRWK=
KOSC-2 NWPz[nNET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnLqTWM2OD1{Lk[zPFc6KM7:TR?= NWCxRoZlW0GQR1XS
TUR NV72S4ZYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2TucmlEPTB;Mj62OVM3QSEQvF2= NECxcnRUSU6JRWK=
NCI-H2087 NWDySFhVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoH5TWM2OD1{LkexOlQ3KM7:TR?= MXHTRW5ITVJ?
SCC-15 NUDLRYJ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\5ZpZKSzVyPUKuO|UzPTJizszN MXHTRW5ITVJ?
HSC-4 NWTOV5ZLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnNTFBSUUN3ME2yMlc5Ozh7IN88US=> NWrpUJlmW0GQR1XS
G-402 MnnVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX\yTlRuUUN3ME2yMlgyPDR5IN88US=> MXHTRW5ITVJ?
GP5d NFLDWnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTJwOEO4Nlch|ryP MVnTRW5ITVJ?
IGR-1 NW\GUlVGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnmwTWM2OD1{LkizPVg4KM7:TR?= MULTRW5ITVJ?
SK-PN-DW NUHRSmRFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUn6OpdzUUN3ME2yMlg3OjB2IN88US=> Mk\sV2FPT0WU
OPM-2 Mn76S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTJwOEm5O|gh|ryP M1z2S3NCVkeHUh?=
5637 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PBdGlEPTB;Mj65NlQ{QSEQvF2= NHj0TnBUSU6JRWK=
NCI-H2030 NHH0U2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33aNWlEPTB;Mj65PFAzQSEQvF2= M{XheXNCVkeHUh?=
SK-LU-1 NHzR[pFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkThTWM2OD1{Lkm4N|M1KM7:TR?= M{HDbHNCVkeHUh?=
EW-18 NInzfpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3pVZpKSzVyPUOuNFU{OiEQvF2= MYTTRW5ITVJ?
786-0 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDhcXZKSzVyPUOuNFU4QDRizszN NVSyS2NLW0GQR1XS
NCI-H1963 NELSPJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTNwMEi4NlUh|ryP M1L4enNCVkeHUh?=
A2780 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHZTnJOUUN3ME2zMlExOzRzIN88US=> MUfTRW5ITVJ?
NCI-H526 Mmf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTNwMUO5OlEh|ryP M37XSnNCVkeHUh?=
BHT-101 NXvRfXFUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3m4V2lEPTB;Mz6xOVc6QCEQvF2= M2[zVnNCVkeHUh?=
RPMI-7951 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LMeWlEPTB;Mz6yNFE{PyEQvF2= M1vPSXNCVkeHUh?=
HL-60 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjOeGZ3UUN3ME2zMlIyOTRizszN NUfQe2Q{W0GQR1XS
CTV-1 NWjUXlR{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\4UI9KSzVyPUOuNlU{PzdizszN NIjzTlJUSU6JRWK=
IST-SL1 NXfwenlFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nLcmlEPTB;Mz6yPVQ3PSEQvF2= M3[ySHNCVkeHUh?=
G-401 NYXmbm17T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlz5TWM2OD1|LkK5PFA5KM7:TR?= MYnTRW5ITVJ?
OVCAR-5 NEfIN|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTNwM{[zOVIh|ryP MnLMV2FPT0WU
EW-1 MmL0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2W3WGlEPTB;Mz60NFU1PCEQvF2= NYjj[JRxW0GQR1XS
H4 M2rBU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEOzSo1KSzVyPUOuOFc2PjlizszN NIW3VI9USU6JRWK=
EM-2 NYTYcHJ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnOzTWM2OD1|LkS4Olc1KM7:TR?= M2HQ[HNCVkeHUh?=
RS4-11 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XZTWlEPTB;Mz60PFcyPSEQvF2= M37XZnNCVkeHUh?=
NOMO-1 MlPqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrJR|UxRTNwNEmyNFQh|ryP NF73[XdUSU6JRWK=
HCT-116 MmLDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTNwNUC3NVEh|ryP M3[0PXNCVkeHUh?=
CAL-120 MoW3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7NTWM2OD1|LkW3N|Uh|ryP MlzDV2FPT0WU
CAL-51 Ml3tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3[yRWlEPTB;Mz61O|YyOiEQvF2= M2XUd3NCVkeHUh?=
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PF-382 NWTZcWllT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfIZ4hKSzVyPUSwMlc1QDdizszN MmHqV2FPT0WU
OAW-42 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUO4[W9XUUN3ME20Nk4zQTR{IN88US=> MmqwV2FPT0WU
LB996-RCC MknuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4S1S2lEPTB;NEKuPFAxQCEQvF2= MlvDV2FPT0WU
SAS NILnNGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUHJR|UxRTR|LkSyPFgh|ryP NEXBVHRUSU6JRWK=
MRK-nu-1 M{j5bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTR2Lke0NVQh|ryP NHPENWdUSU6JRWK=
EoL-1-cell MnzMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFq3flFKSzVyPUS0MlkyOzZizszN MXnTRW5ITVJ?
NCI-H2342 NEj6SpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTR3LkGxNVch|ryP NV7pcVE1W0GQR1XS
NCI-H1703 NYKxeYxoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mke1TWM2OD12Nj6wOVkzKM7:TR?= MljxV2FPT0WU
NCI-H187 Mmn5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nSOWlEPTB;NE[uNVg3QSEQvF2= NHTndnZUSU6JRWK=
CP66-MEL M{\rWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTR4LkK3PFkh|ryP MY\TRW5ITVJ?
HCC1569 NWrScZB5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLvTWM2OD12Nj61PVE4KM7:TR?= M1[5W3NCVkeHUh?=
BL-70 NHzlVWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTR4Lke3N|ch|ryP NFnjXlRUSU6JRWK=
Saos-2 M3\WNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkL4TWM2OD12Nz6zNVI{KM7:TR?= NVvaenVCW0GQR1XS
LC-1F NX\k[GFRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnG3TWM2OD12Nz62NVA2KM7:TR?= NETYepZUSU6JRWK=
KY821 MkT0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIDCNmdKSzVyPUS3MlY1OzRizszN M{PmVXNCVkeHUh?=
PSN1 Mk\yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGe1fYRKSzVyPUS4MlE1ODFizszN MYjTRW5ITVJ?
RT4 MknUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTR6LkizO|ch|ryP Ml\TV2FPT0WU
LS-1034 NFrEWWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHVTWM2OD12OD64PVU1KM7:TR?= NInOWotUSU6JRWK=
NCI-H1395 NV22OGZyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;ESJRKSzVyPUS5MlA1QTJizszN NYC3UZE5W0GQR1XS
EKVX NH\DSFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTR7Lk[3N|Eh|ryP M{jnfnNCVkeHUh?=
IST-MES1 Ml;5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTR7Lki5OFQh|ryP Mn7EV2FPT0WU

... Click to View More Cell Line Experimental Data

In vivo PF-562271 (< 33 mg/kg p.o.) inhibits FAK phosphorylation in tumors in a dose- and time-dependent manner in U87MG-bearing mice. PF-562271 (50 mg/kg p.o. bid) results in 86% tumor growth inhibition in BxPc3 xenografts mice and 45% tumor growth inhibition in PC3-M xenografts mice. PF-562271 (25 mg/kg, bid) results in 2-fold greater apoptosis in treated tumors in mice bearing H125 lung xenografts. [1] PF-562271 (33 mg/kg, p.o.) inhibits extensive movement of the tumor cells over 24 hours in mice. PF-562271 (33 mg/kg, p.o.) results in altered E-cadherin dynamics in mice, which correlates with reduced E-cadherin–dependent collective cell movement. [3] PF-562271 (25 mg/kg, p.o. bid) results in 62% tumor growth inhibition in PC3M-luc-C6 subcutaneuous local implant xenograft mouse model. [4] PF-562,271 (5 mg/kg, oral) results in significant and similar increases in osteocalcin and cancellous bone parameters and a decrease in tumor growth and signs of bone healing in rats implanted with MDA-MB-231 cells in the tibia. [5]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Athymic female mice bearing BxPc3 or PC3-M xenografts.
  • Formulation: 5% Gelucire
  • Dosages: 50 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (197.04 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.49
Formula

C21H20F3N7O3S

CAS No. 717907-75-0
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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FAK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID