PF-562271

Catalog No.S2890

PF-562271 Chemical Structure

Molecular Weight(MW): 507.49

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.

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In DMSO USD 350 In stock
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4 Customer Reviews

  • (F,G) The migration and invasion ability of 5637 and T24 cells transfected with PF-562271 and WT-METTL13 was examined. The number of cells was quantified and is shown in a column graph.

    Sci Rep, 2016, 6:19261. PF-562271 purchased from Selleck.

    D-F. T47D cells plated as in A were treated with vehicle (−) or the FAK Y397 inhibitor, PF-562271 (+), for 1 h prior to ± PRL (4 nM) for 15 min (representative immunoblots).

    Oncotarget, 2016, 7(30):48093-48106. PF-562271 purchased from Selleck.

  • OVISE cells were incubated for 24 hr at the indicated concentrations of the FAK inhibitors. Immunoblots were performed to assess inhibition of auto-phosphorylation by the FAK inhibitors.

    PLoS One 2014 9(2), e88587. PF-562271 purchased from Selleck.

    VEGF and P450 enzyme production in response to FAK or Src inhibitors. MCF10A cells were cultured in LD collagen gels ?vehicle control (V), 10 uM PF-562271 FAK ATP inhibitor (PF) or Src inhibitor PP2 for 24 h. Cells were also lysed and examined for CYP1A1 or CYP4B1 by immunoblot (B and C) and densitometry (D and E). VEGF data are displayed as the mean normalized to the vehicle control, N = 6, 盨EM, *p < 0.003 vs control (A). P450 data are displayed as the mean relative to GAPDH and normalized to the control. N > 4, ±SEM, *p < 0.05 vs respective control (D and E).

    Environ Toxicol Pharmacol 2014 39(1), 114-124. PF-562271 purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 binds in the ATP-binding cleft of FAK, forming two of the three “canonical” H-bonds between the inhibitor and main-chain atoms in the kinase hinge region. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with IC50 of 5 nM. PF-562271 (3.3 μM) results in G1 arrest of PC3-M cells. [1] PF-562271 (1 nM) blocks bFGF-stimulated blood vessel angiogenesis as performed in chicken chorioallantoic membrane assays. PF-262271 potently blocks blood vessel sprouting without detectable changes in vascular leakage. [2] PF-562271 (250 nM) results in complete inhibition of collective A431 cell invasion into collagen gels. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 NYLsNVJOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\zZmlEPTB;MD6yO|Y3KM7:TR?= M{LGRXNCVkeHUh?=
SW982 NV3hfHlbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkK2TWM2OD1yLkOyPFIh|ryP M4fSNXNCVkeHUh?=
KM12 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofXTWM2OD1yLkO4OVU4KM7:TR?= MWfTRW5ITVJ?
COLO-205 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1GxVmlEPTB;MD60PFY2QCEQvF2= NHXW[ZFUSU6JRWK=
COLO-829 M3rSOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH22VYtKSzVyPUCuO|UyPzZizszN NViwdmdzW0GQR1XS
MG-63 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zyOmlEPTB;MD64NFY{PyEQvF2= NX3GOpoxW0GQR1XS
IGROV-1 M4X2O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\NcGZnUUN3ME2wMlgyODN6IN88US=> NWO5O3NUW0GQR1XS
NCI-H650 MnT3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGWxNY1KSzVyPUCuPFMyPTRizszN M2XLZnNCVkeHUh?=
RT-112 NXjkNFFbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTBwOUi0OkDPxE1? NH\WcWNUSU6JRWK=
BCPAP MkPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnyOnVIUUN3ME2xMlAyOjh6IN88US=> NGHEeFRUSU6JRWK=
ALL-PO NWXXV|VmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF[3[Y9KSzVyPUGuNFE2QDRizszN NUiw[Go3W0GQR1XS
KYSE-270 NX24UIg1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zuSmlEPTB;MT6wOFcyPCEQvF2= NHjhZY9USU6JRWK=
8305C NWfD[GVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3j5R2lEPTB;MT6wPVkxPCEQvF2= NHe4Om1USU6JRWK=
NCI-H810 NWH6WY9bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUG1dnZnUUN3ME2xMlExPzd4IN88US=> NIKwXnVUSU6JRWK=
CAL-33 NI\GdFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYq3OJd[UUN3ME2xMlEzQTN6IN88US=> NHWwfnJUSU6JRWK=
AN3-CA MoG2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjhd3lXUUN3ME2xMlIyQDZ5IN88US=> Mmf1V2FPT0WU
NKM-1 NVfGV5M2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX22dYdxUUN3ME2xMlI4PTB4IN88US=> NXrhS5F2W0GQR1XS
BPH-1 NET2epdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjYU5ZKSzVyPUGuNlg4PjZizszN NXnyOYRMW0GQR1XS
MES-SA MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkLkTWM2OD1zLkOwOlgzKM7:TR?= MWfTRW5ITVJ?
CAL-62 Ml3VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4n3fGlEPTB;MT6zNVkxQSEQvF2= NYnJdmtvW0GQR1XS
Detroit562 M3r6Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnITWM2OD1zLkO1NlIzKM7:TR?= MWDTRW5ITVJ?
KYSE-150 NUD3TGJmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTFwM{WyN|Yh|ryP NEjkO5RUSU6JRWK=
SK-UT-1 NFXWfXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTFwNES2OFch|ryP MVjTRW5ITVJ?
HUTU-80 NEPUTmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\x[m9KSzVyPUGuOFQ5QDZizszN NIHDXWFUSU6JRWK=
SIG-M5 NXjKS25mT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\MXFZ6UUN3ME2xMlQ5Ozh5IN88US=> MnTPV2FPT0WU
AGS NHrrXJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTFwNUKxNlQh|ryP NFXNR5lUSU6JRWK=
ST486 NFTEeWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTiOGtxUUN3ME2xMlU{Ojd6IN88US=> NF\l[GtUSU6JRWK=
HSC-2 M1fEOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nLW2lEPTB;MT61N|k2KM7:TR?= MYnTRW5ITVJ?
LCLC-103H NVH0O3gxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTFwNUW4NVgh|ryP NInDNo5USU6JRWK=
BC-1 NHK3NnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjFUmpqUUN3ME2xMlYyPjZ2IN88US=> NI\UNHZUSU6JRWK=
CGTH-W-1 MmnzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fQVGlEPTB;MT62NVY4QSEQvF2= M2f0fXNCVkeHUh?=
MZ1-PC MonoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2DDNmlEPTB;MT62NlMyOiEQvF2= M3fkdnNCVkeHUh?=
SW1710 NYjyXW11T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTFwNkK2Nlgh|ryP MWfTRW5ITVJ?
EW-13 NV;ze4VYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;pd4pKSzVyPUGuOlM1PjZizszN M17adXNCVkeHUh?=
TE-11 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPBOodKSzVyPUGuOlM2OjJizszN NHO2dWZUSU6JRWK=
BHY NH3FV2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XNS2lEPTB;MT62OVY3OyEQvF2= MVzTRW5ITVJ?
ML-2 NHvobmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXQUJdKSzVyPUGuOlY5ODJizszN NELX[ZFUSU6JRWK=
MEL-JUSO NYrXVVJuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrwfFBzUUN3ME2xMlczQDlzIN88US=> M3TERXNCVkeHUh?=
U251 M1rtVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XSXmlEPTB;MT63OFA{OSEQvF2= MkGzV2FPT0WU
MHH-ES-1 M4LtRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTFwN{m1Olgh|ryP MWfTRW5ITVJ?
CAL-85-1 Mn;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjoPWJlUUN3ME2xMlgxPDlizszN M1;EOHNCVkeHUh?=
D-336MG MnTIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnT6TWM2OD1zLki1OlE1KM7:TR?= M1TRN3NCVkeHUh?=
VA-ES-BJ MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPCPJdWUUN3ME2xMlkxOzF3IN88US=> NF;jVXRUSU6JRWK=
MDA-MB-468 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYKwOmh7UUN3ME2xMlkyOjd{IN88US=> MV;TRW5ITVJ?
MEL-HO M4DvPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3H6OmlEPTB;MT65O|k6OyEQvF2= Ml\GV2FPT0WU
SW954 M2Tn[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTJwMECxOVEh|ryP NWXMNVVxW0GQR1XS
LB831-BLC NFXSUW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTJwMEC5PFgh|ryP NUm3T3NVW0GQR1XS
EW-16 M37lWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX21RWVRUUN3ME2yMlA{QTh|IN88US=> NH;VRXlUSU6JRWK=
NCI-H460 MoflS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\HZXNKSzVyPUKuNFQ5OzlizszN NX:1[Io6W0GQR1XS
CHP-212 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mme2TWM2OD1{LkC5OVM6KM7:TR?= NYS5UHp6W0GQR1XS
MHH-NB-11 NFPpUoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PxNmlEPTB;Mj6xNlgyQCEQvF2= MY\TRW5ITVJ?
DU-4475 MoTJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEmzNZJKSzVyPUKuNVQ4PTlizszN MXTTRW5ITVJ?
ABC-1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTJwMkWxPVYh|ryP NF;FcGVUSU6JRWK=
LOUCY MonnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITMO|dKSzVyPUKuNlYyOjhizszN MXjTRW5ITVJ?
LC-2-ad MkjWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTw[4VvUUN3ME2yMlI3QTZ4IN88US=> NFLxXG1USU6JRWK=
ETK-1 M2LQeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlTSTWM2OD1{LkK4OFYh|ryP MlnxV2FPT0WU
KYSE-180 MnqyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfoWWVKSzVyPUKuNlg5PzRizszN NHTEenBUSU6JRWK=
HOP-92 M{njeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTJwM{C2O|Mh|ryP MWLTRW5ITVJ?
RCC10RGB MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFu1epNKSzVyPUKuN|I2ODdizszN MkPpV2FPT0WU
KASUMI-1 NWDWT4p5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7kdldzUUN3ME2yMlMzQDhizszN NHG3TY9USU6JRWK=
ACN Mki5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTJwM{S3OFUh|ryP NWXnTHE3W0GQR1XS
BxPC-3 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vtN2lEPTB;Mj6zOVQ{PiEQvF2= M3LwZXNCVkeHUh?=
MONO-MAC-6 NYfTcml{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTJwNEGyOFgh|ryP NED5TIlUSU6JRWK=
CAL-39 M1:xfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTJwNEG2Nlgh|ryP NYTM[WI2W0GQR1XS
CMK MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzQcWc2UUN3ME2yMlQyPzl3IN88US=> MU\TRW5ITVJ?
MFE-296 Ml3rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXf2T3Y{UUN3ME2yMlQ4Pzl{IN88US=> M1nBeHNCVkeHUh?=
DU-145 MonJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoraTWM2OD1{LkS5NVE5KM7:TR?= M{\FU3NCVkeHUh?=
MDA-MB-231 M1v3cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nFTWlEPTB;Mj60PVU4OiEQvF2= MX\TRW5ITVJ?
EFO-27 NVT6V3Z6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjyco53UUN3ME2yMlUyQDh6IN88US=> MnvKV2FPT0WU
SNU-387 M1;pc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfucpN{UUN3ME2yMlUzQDJizszN M2fYW3NCVkeHUh?=
KYSE-410 NU\5bVN[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTJwNU[1NVch|ryP NV;lT|JtW0GQR1XS
NCCIT MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XHS2lEPTB;Mj61O|I1KM7:TR?= MljsV2FPT0WU
LXF-289 NX;4SHN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jPUWlEPTB;Mj61PFI4OyEQvF2= NYTCO3JrW0GQR1XS
HMV-II MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTCTWM2OD1{LkW4PVk4KM7:TR?= MXjTRW5ITVJ?
MOLT-4 MmXRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTJwNkC3NVIh|ryP NHriW4lUSU6JRWK=
KOSC-2 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHjTWM2OD1{Lk[zPFc6KM7:TR?= MWnTRW5ITVJ?
TUR NYrIbHY1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHQTWM2OD1{Lk[1N|Y6KM7:TR?= NVvR[JZEW0GQR1XS
NCI-H2087 NXPubXR6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3PQNGlEPTB;Mj63NVY1PiEQvF2= M3XIRnNCVkeHUh?=
SCC-15 M4fZcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{ntSGlEPTB;Mj63OVI2OiEQvF2= MoL2V2FPT0WU
HSC-4 NUniVVA1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkmyTWM2OD1{Lke4N|g6KM7:TR?= NWDvd|NYW0GQR1XS
G-402 NIKyS2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXxTWM2OD1{LkixOFQ4KM7:TR?= MkO2V2FPT0WU
GP5d MkTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nIXmlEPTB;Mj64N|gzPyEQvF2= MnzJV2FPT0WU
IGR-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDLTWM2OD1{LkizPVg4KM7:TR?= M{D1OXNCVkeHUh?=
SK-PN-DW NF;z[JdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTESWxWUUN3ME2yMlg3OjB2IN88US=> MorMV2FPT0WU
OPM-2 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTJwOEm5O|gh|ryP NYD1TG44W0GQR1XS
5637 NUH5eYd4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfRTWM2OD1{LkmyOFM6KM7:TR?= MnnXV2FPT0WU
NCI-H2030 M2PoXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnS4TWM2OD1{Lkm4NFI6KM7:TR?= NVHWelY6W0GQR1XS
SK-LU-1 NXm0Z4NWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M16x[mlEPTB;Mj65PFM{PCEQvF2= MUXTRW5ITVJ?
EW-18 M3;xN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFOydoVKSzVyPUOuNFU{OiEQvF2= MUPTRW5ITVJ?
786-0 MofxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\HbZZKSzVyPUOuNFU4QDRizszN M2j1bXNCVkeHUh?=
NCI-H1963 Ml:wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmPjTWM2OD1|LkC4PFI2KM7:TR?= NYfhWXFzW0GQR1XS
A2780 NWjnW|ZST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\QTWM2OD1|LkGwN|QyKM7:TR?= MnvVV2FPT0WU
NCI-H526 M4TqW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHv0cXdKSzVyPUOuNVM6PjFizszN M{e3cXNCVkeHUh?=
BHT-101 MkXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDESFVKSzVyPUOuNVU4QThizszN NXfqUpoyW0GQR1XS
RPMI-7951 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2n5eWlEPTB;Mz6yNFE{PyEQvF2= MYDTRW5ITVJ?
HL-60 MljHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGjsNZVKSzVyPUOuNlEyPCEQvF2= NYHpXG94W0GQR1XS
CTV-1 MnLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXO2S|dOUUN3ME2zMlI2Ozd5IN88US=> NFn0dYRUSU6JRWK=
IST-SL1 M3nLfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXsTWM2OD1|LkK5OFY2KM7:TR?= NEH4NIhUSU6JRWK=
G-401 M4ew[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\Ce3FGUUN3ME2zMlI6QDB6IN88US=> Mn3FV2FPT0WU
OVCAR-5 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTNwM{[zOVIh|ryP NVjHNow{W0GQR1XS
EW-1 NGjndotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTNwNEC1OFQh|ryP NX\z[lE1W0GQR1XS
H4 NYrLSHNMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrGWW1KSzVyPUOuOFc2PjlizszN M2i3cHNCVkeHUh?=
EM-2 NUPvPJRJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTNwNEi2O|Qh|ryP NUTTTZNlW0GQR1XS
RS4-11 NVPMZ|RNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTNwNEi3NVUh|ryP Mkf0V2FPT0WU
NOMO-1 NGDVS2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYD3UmU{UUN3ME2zMlQ6OjB2IN88US=> NX[wOGRvW0GQR1XS
HCT-116 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3znUWlEPTB;Mz61NFcyOSEQvF2= M4L5[XNCVkeHUh?=
CAL-120 MnrnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTNwNUezOUDPxE1? MWXTRW5ITVJ?
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PF-382 Mo[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\FbWlEPTB;NECuO|Q5PyEQvF2= M4P4OnNCVkeHUh?=
OAW-42 NEfz[21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWexSY5[UUN3ME20Nk4zQTR{IN88US=> NE\IXo1USU6JRWK=
LB996-RCC MknrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTR{LkiwNFgh|ryP Moe3V2FPT0WU
SAS MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfzXXNoUUN3ME20N{41Ojh6IN88US=> MmnJV2FPT0WU
MRK-nu-1 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofJTWM2OD12ND63OFE1KM7:TR?= NYe3Z|g{W0GQR1XS
EoL-1-cell MkX6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn:2TWM2OD12ND65NVM3KM7:TR?= MlfLV2FPT0WU
NCI-H2342 M{PBUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTR3LkGxNVch|ryP MU\TRW5ITVJ?
NCI-H1703 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PLZWlEPTB;NE[uNFU6OiEQvF2= MWLTRW5ITVJ?
NCI-H187 NGewb5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoG2TWM2OD12Nj6xPFY6KM7:TR?= MnvRV2FPT0WU
CP66-MEL NGjCVnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrsfotKSzVyPUS2MlI4QDlizszN NH3PcG9USU6JRWK=
HCC1569 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoH2TWM2OD12Nj61PVE4KM7:TR?= MUXTRW5ITVJ?
BL-70 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITwUo9KSzVyPUS2Mlc4OzdizszN NH\mb|ZUSU6JRWK=
Saos-2 NVPJOJpvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHe0ellKSzVyPUS3MlMyOjNizszN MVTTRW5ITVJ?
LC-1F NY\uN25[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTR5Lk[xNFUh|ryP MXjTRW5ITVJ?
KY821 NVTtNVBMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTR5Lk[0N|Qh|ryP NF[3WpdUSU6JRWK=
PSN1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFrrTVRKSzVyPUS4MlE1ODFizszN NYT0VYVlW0GQR1XS
RT4 NVrKbZNmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\QXIxKSzVyPUS4Mlg{PzdizszN NXSyZ2x[W0GQR1XS
LS-1034 NF\XS|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUnJR|UxRTR6Lki5OVQh|ryP MlqyV2FPT0WU
NCI-H1395 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljaTWM2OD12OT6wOFkzKM7:TR?= NVvkfXZrW0GQR1XS
EKVX M3jS[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXT1NmxQUUN3ME20PU43PzNzIN88US=> NUjjbo5lW0GQR1XS
IST-MES1 M2n1Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYK2cnpbUUN3ME20PU45QTR2IN88US=> M2XrcXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo PF-562271 (< 33 mg/kg p.o.) inhibits FAK phosphorylation in tumors in a dose- and time-dependent manner in U87MG-bearing mice. PF-562271 (50 mg/kg p.o. bid) results in 86% tumor growth inhibition in BxPc3 xenografts mice and 45% tumor growth inhibition in PC3-M xenografts mice. PF-562271 (25 mg/kg, bid) results in 2-fold greater apoptosis in treated tumors in mice bearing H125 lung xenografts. [1] PF-562271 (33 mg/kg, p.o.) inhibits extensive movement of the tumor cells over 24 hours in mice. PF-562271 (33 mg/kg, p.o.) results in altered E-cadherin dynamics in mice, which correlates with reduced E-cadherin–dependent collective cell movement. [3] PF-562271 (25 mg/kg, p.o. bid) results in 62% tumor growth inhibition in PC3M-luc-C6 subcutaneuous local implant xenograft mouse model. [4] PF-562,271 (5 mg/kg, oral) results in significant and similar increases in osteocalcin and cancellous bone parameters and a decrease in tumor growth and signs of bone healing in rats implanted with MDA-MB-231 cells in the tibia. [5]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Athymic female mice bearing BxPc3 or PC3-M xenografts.
  • Formulation: 5% Gelucire
  • Dosages: 50 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (197.04 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.49
Formula

C21H20F3N7O3S

CAS No. 717907-75-0
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    What’s the difference between S2890 and S2672?

  • Answer:

    S2890 is free base and S2672 is the besylate salt, S2672 has better solubility than S2890.

FAK Signaling Pathway Map

Related FAK Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID