PF-562271

Catalog No.S2890

PF-562271 Chemical Structure

Molecular Weight(MW): 507.49

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.

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In DMSO USD 350 In stock
USD 170 In stock
USD 270 In stock
USD 670 In stock
USD 970 In stock
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5 Customer Reviews

  • Effect of matrix stiffness on the susceptibility of primary HDFs to apoptosis induced by inhibition of mechanotransduction pathways. HDFs (primary human dermal fibroblasts) were cultured on collagen-coated polyacrylamide hydrogels that recapitulated the stiffness of normal (4 kPa) or densely fibrotic (50 kPa) skin for 24 hours and treated with or without the agents indicated for an additional 48 hours. Apoptosis was assessed by annexin V staining. Data are means ± SD from three independent experiments. P value was determined by Student’s t test.

    Science, 2017, 9(420), doi: 10.1126/scitranslmed.aal3765. PF-562271 purchased from Selleck.

    (F,G) The migration and invasion ability of 5637 and T24 cells transfected with PF-562271 and WT-METTL13 was examined. The number of cells was quantified and is shown in a column graph.

    Sci Rep, 2016, 6:19261. PF-562271 purchased from Selleck.

  • D-F. T47D cells plated as in A were treated with vehicle (−) or the FAK Y397 inhibitor, PF-562271 (+), for 1 h prior to ± PRL (4 nM) for 15 min (representative immunoblots).

    Oncotarget, 2016, 7(30):48093-48106. PF-562271 purchased from Selleck.

    OVISE cells were incubated for 24 hr at the indicated concentrations of the FAK inhibitors. Immunoblots were performed to assess inhibition of auto-phosphorylation by the FAK inhibitors.

    PLoS One 2014 9(2), e88587. PF-562271 purchased from Selleck.

  • VEGF and P450 enzyme production in response to FAK or Src inhibitors. MCF10A cells were cultured in LD collagen gels ?vehicle control (V), 10 uM PF-562271 FAK ATP inhibitor (PF) or Src inhibitor PP2 for 24 h. Cells were also lysed and examined for CYP1A1 or CYP4B1 by immunoblot (B and C) and densitometry (D and E). VEGF data are displayed as the mean normalized to the vehicle control, N = 6, 盨EM, *p < 0.003 vs control (A). P450 data are displayed as the mean relative to GAPDH and normalized to the control. N > 4, ±SEM, *p < 0.05 vs respective control (D and E).

    Environ Toxicol Pharmacol 2014 39(1), 114-124. PF-562271 purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 binds in the ATP-binding cleft of FAK, forming two of the three “canonical” H-bonds between the inhibitor and main-chain atoms in the kinase hinge region. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with IC50 of 5 nM. PF-562271 (3.3 μM) results in G1 arrest of PC3-M cells. [1] PF-562271 (1 nM) blocks bFGF-stimulated blood vessel angiogenesis as performed in chicken chorioallantoic membrane assays. PF-262271 potently blocks blood vessel sprouting without detectable changes in vascular leakage. [2] PF-562271 (250 nM) results in complete inhibition of collective A431 cell invasion into collagen gels. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 NFnxZZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjyPXNKSzVyPUCuNlc3PiEQvF2= M{PpUnNCVkeHUh?=
SW982 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfiSnpKSzVyPUCuN|I5OiEQvF2= M4PPWXNCVkeHUh?=
KM12 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHv5dXpKSzVyPUCuN|g2PTdizszN MkSyV2FPT0WU
COLO-205 NFrWT3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TMR2lEPTB;MD60PFY2QCEQvF2= MVzTRW5ITVJ?
COLO-829 NIjxNnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XCVWlEPTB;MD63OVE4PiEQvF2= NGXW[|NUSU6JRWK=
MG-63 NI\qcGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXe3O4JHUUN3ME2wMlgxPjN5IN88US=> MWDTRW5ITVJ?
IGROV-1 Mk\qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojHTWM2OD1yLkixNFM5KM7:TR?= MYDTRW5ITVJ?
NCI-H650 MoH2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\sW4VKSzVyPUCuPFMyPTRizszN NXzkT5k{W0GQR1XS
RT-112 NIWzVmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWLqbGVYUUN3ME2wMlk5PDZizszN NYDhdlI6W0GQR1XS
BCPAP MkH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTFwMEGyPFgh|ryP MojSV2FPT0WU
ALL-PO M4DHOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rTd2lEPTB;MT6wNVU5PCEQvF2= MWfTRW5ITVJ?
KYSE-270 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4jnTmlEPTB;MT6wOFcyPCEQvF2= M1jiPXNCVkeHUh?=
8305C NGXh[m1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jyd2lEPTB;MT6wPVkxPCEQvF2= NG\hOItUSU6JRWK=
NCI-H810 NID0OHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLlfG5{UUN3ME2xMlExPzd4IN88US=> MUPTRW5ITVJ?
CAL-33 NI[3cHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTFwMUK5N|gh|ryP MYLTRW5ITVJ?
AN3-CA MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTFwMkG4Olch|ryP MlPCV2FPT0WU
NKM-1 NIfGenRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\aS2lEPTB;MT6yO|UxPiEQvF2= MlTCV2FPT0WU
BPH-1 NELrPZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHRZ|RDUUN3ME2xMlI5PzZ4IN88US=> NELMV4pUSU6JRWK=
MES-SA NXTWZY4xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrjSFd[UUN3ME2xMlMxPjh{IN88US=> NX3l[nZwW0GQR1XS
CAL-62 NFO1e|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXqZnpWUUN3ME2xMlMyQTB7IN88US=> M4LURnNCVkeHUh?=
Detroit562 NFPvcZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkH2TWM2OD1zLkO1NlIzKM7:TR?= NWH3RW9sW0GQR1XS
KYSE-150 M{XRemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnf5TWM2OD1zLkO1NlM3KM7:TR?= M1u4b3NCVkeHUh?=
SK-UT-1 M2XFd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PINmlEPTB;MT60OFY1PyEQvF2= NET0OIhUSU6JRWK=
HUTU-80 NU\nNpVLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{i1c2lEPTB;MT60OFg5PiEQvF2= NYrS[oZQW0GQR1XS
SIG-M5 NGr6c3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4CyPWlEPTB;MT60PFM5PyEQvF2= M4[1enNCVkeHUh?=
AGS MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml24TWM2OD1zLkWyNVI1KM7:TR?= NIfBRmZUSU6JRWK=
ST486 MkfXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjybGpKUUN3ME2xMlU{Ojd6IN88US=> MnvqV2FPT0WU
HSC-2 NHfiTJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLQUYVoUUN3ME2xMlU{QTVizszN NIS2[WdUSU6JRWK=
LCLC-103H MmLyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7rZ|RKSzVyPUGuOVU5OThizszN MWrTRW5ITVJ?
BC-1 NVTWb5dCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M176NmlEPTB;MT62NVY3PCEQvF2= NEnH[|FUSU6JRWK=
CGTH-W-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTFwNkG2O|kh|ryP NWPGcphjW0GQR1XS
MZ1-PC NWS3PJhuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rq[WlEPTB;MT62NlMyOiEQvF2= NITHcZZUSU6JRWK=
SW1710 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjkUYtXUUN3ME2xMlYzPjJ6IN88US=> NETCfYRUSU6JRWK=
EW-13 NV3OfFBMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHP3eWZKSzVyPUGuOlM1PjZizszN Mlr4V2FPT0WU
TE-11 M1SxWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTFwNkO1NlIh|ryP M4rvXnNCVkeHUh?=
BHY MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jzbGlEPTB;MT62OVY3OyEQvF2= MVjTRW5ITVJ?
ML-2 MmT2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml6zTWM2OD1zLk[2PFAzKM7:TR?= MUPTRW5ITVJ?
MEL-JUSO NFXMfm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1S0NWlEPTB;MT63Nlg6OSEQvF2= Ml;GV2FPT0WU
U251 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NICyd5RKSzVyPUGuO|QxOzFizszN MoPRV2FPT0WU
MHH-ES-1 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTFwN{m1Olgh|ryP NHq1cnNUSU6JRWK=
CAL-85-1 M1P6eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7he5FXUUN3ME2xMlgxPDlizszN MnPlV2FPT0WU
D-336MG NVPCN3VmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknzTWM2OD1zLki1OlE1KM7:TR?= NE[xdJNUSU6JRWK=
VA-ES-BJ M4WwfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnuTWM2OD1zLkmwN|E2KM7:TR?= NXLoPFBvW0GQR1XS
MDA-MB-468 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHVUFRKSzVyPUGuPVEzPzJizszN MnfBV2FPT0WU
MEL-HO NV7R[pd5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIruR|hKSzVyPUGuPVc6QTNizszN MVnTRW5ITVJ?
SW954 NIfRbpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfKWIRlUUN3ME2yMlAxOTVzIN88US=> NEHnXVhUSU6JRWK=
LB831-BLC NEDCVlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2T0Z2lEPTB;Mj6wNFk5QCEQvF2= MX;TRW5ITVJ?
EW-16 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTITWM2OD1{LkCzPVg{KM7:TR?= NFzQVnJUSU6JRWK=
NCI-H460 Mnr6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHmzUFVKSzVyPUKuNFQ5OzlizszN Mke1V2FPT0WU
CHP-212 NET2bohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\BXpdKSzVyPUKuNFk2OzlizszN MWnTRW5ITVJ?
MHH-NB-11 NHO4VohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDrbnhKSzVyPUKuNVI5OThizszN NUTL[Zl2W0GQR1XS
DU-4475 M1vWbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M323WmlEPTB;Mj6xOFc2QSEQvF2= M1\6NXNCVkeHUh?=
ABC-1 M3rmR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLrZ4NGUUN3ME2yMlI2OTl4IN88US=> NUPTbo1EW0GQR1XS
LOUCY MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTJwMk[xNlgh|ryP M1:wW3NCVkeHUh?=
LC-2-ad NWj3NGFsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7sTWM2OD1{LkK2PVY3KM7:TR?= MV7TRW5ITVJ?
ETK-1 NInrd4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTJwMki0OkDPxE1? NWfB[4F[W0GQR1XS
KYSE-180 NY[3VFhOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LsbGlEPTB;Mj6yPFg4PCEQvF2= NEG0epRUSU6JRWK=
HOP-92 Mn:5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3T6OWlEPTB;Mj6zNFY4OyEQvF2= NInHSppUSU6JRWK=
RCC10RGB MnPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTJwM{K1NFch|ryP MkP6V2FPT0WU
KASUMI-1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13rR2lEPTB;Mj6zNlg5KM7:TR?= M3XEZXNCVkeHUh?=
ACN NFXoV5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvKTWM2OD1{LkO0O|Q2KM7:TR?= NVywdWhUW0GQR1XS
BxPC-3 NEnxfpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTJwM{W0N|Yh|ryP M{S2[XNCVkeHUh?=
MONO-MAC-6 NUPaWWJlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrkTWM2OD1{LkSxNlQ5KM7:TR?= MV;TRW5ITVJ?
CAL-39 NWjJTZJZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjuVFlKSzVyPUKuOFE3OjhizszN NGDGd3pUSU6JRWK=
CMK Mle1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MorBTWM2OD1{LkSxO|k2KM7:TR?= NFy3UGNUSU6JRWK=
MFE-296 NVTCXmdtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTJwNEe3PVIh|ryP M4rxfHNCVkeHUh?=
DU-145 NXHpXHY5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFHoe|RKSzVyPUKuOFkyOThizszN MUTTRW5ITVJ?
MDA-MB-231 MmjJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWH1ToZnUUN3ME2yMlQ6PTd{IN88US=> MV;TRW5ITVJ?
EFO-27 NFfWNmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnSWGhRUUN3ME2yMlUyQDh6IN88US=> MV\TRW5ITVJ?
SNU-387 MkP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTJwNUK4NkDPxE1? MoXWV2FPT0WU
KYSE-410 M2exNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUHBT3JUUUN3ME2yMlU3PTF5IN88US=> NWLD[mVFW0GQR1XS
NCCIT M1jnOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTJwNUeyOEDPxE1? Ml7RV2FPT0WU
LXF-289 M1\veWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkH4TWM2OD1{LkW4Nlc{KM7:TR?= Mn;2V2FPT0WU
HMV-II MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTJwNUi5PVch|ryP MkjnV2FPT0WU
MOLT-4 MlXjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTJwNkC3NVIh|ryP MnPMV2FPT0WU
KOSC-2 NWrLOI9bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1z0XWlEPTB;Mj62N|g4QSEQvF2= MYXTRW5ITVJ?
TUR MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrnTWM2OD1{Lk[1N|Y6KM7:TR?= NEP3clJUSU6JRWK=
NCI-H2087 M1S1cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjzXYRIUUN3ME2yMlcyPjR4IN88US=> MWfTRW5ITVJ?
SCC-15 NVi1O3VlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHPNnR5UUN3ME2yMlc2OjV{IN88US=> MUnTRW5ITVJ?
HSC-4 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;ITWM2OD1{Lke4N|g6KM7:TR?= NH[4dWpUSU6JRWK=
G-402 NVXWXHY6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvO[lZKSzVyPUKuPFE1PDdizszN NWXER4ZDW0GQR1XS
GP5d Mn\uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnYNG82UUN3ME2yMlg{QDJ5IN88US=> M{TpW3NCVkeHUh?=
IGR-1 NG\n[WFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELNOG9KSzVyPUKuPFM6QDdizszN MmPLV2FPT0WU
SK-PN-DW NV7oeZJmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTJwOE[yNFQh|ryP NEjGUmlUSU6JRWK=
OPM-2 NFfSO4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTJwOEm5O|gh|ryP MWPTRW5ITVJ?
5637 M3PseGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTJwOUK0N|kh|ryP NETRe5dUSU6JRWK=
NCI-H2030 MkHES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3nPcmlEPTB;Mj65PFAzQSEQvF2= NV61elV4W0GQR1XS
SK-LU-1 NEHZXG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHoeVc5UUN3ME2yMlk5OzN2IN88US=> MXvTRW5ITVJ?
EW-18 M2XDb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTNwMEWzNkDPxE1? NF3sSYNUSU6JRWK=
786-0 NGDwVlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfOTm1KSzVyPUOuNFU4QDRizszN MWnTRW5ITVJ?
NCI-H1963 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjBXIVKSzVyPUOuNFg5OjVizszN M{e4fnNCVkeHUh?=
A2780 M1ryZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{j4UWlEPTB;Mz6xNFM1OSEQvF2= NXvoXFl5W0GQR1XS
NCI-H526 NXLx[ZZiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLUTWM2OD1|LkGzPVYyKM7:TR?= M1\PfnNCVkeHUh?=
BHT-101 MkHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTNwMUW3PVgh|ryP M161fHNCVkeHUh?=
RPMI-7951 Mkm3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7iTWM2OD1|LkKwNVM4KM7:TR?= NF\DbIZUSU6JRWK=
HL-60 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHqNVJ{UUN3ME2zMlIyOTRizszN NGXMemxUSU6JRWK=
CTV-1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX:wd|JHUUN3ME2zMlI2Ozd5IN88US=> M1;nc3NCVkeHUh?=
IST-SL1 NF3Lb29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTNwMkm0OlUh|ryP M2\5TnNCVkeHUh?=
G-401 NXi4bnd{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M36zXGlEPTB;Mz6yPVgxQCEQvF2= M17SV3NCVkeHUh?=
OVCAR-5 NUjkfZFnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTNwM{[zOVIh|ryP NYGwcmYyW0GQR1XS
EW-1 NHvae|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jGcWlEPTB;Mz60NFU1PCEQvF2= NHzl[2hUSU6JRWK=
H4 M17KWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFG1O5NKSzVyPUOuOFc2PjlizszN MUfTRW5ITVJ?
EM-2 M1rIUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7SNlhKSzVyPUOuOFg3PzRizszN NUHVXo5YW0GQR1XS
RS4-11 NYfkd2lHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1j1NGlEPTB;Mz60PFcyPSEQvF2= NY\tflRpW0GQR1XS
NOMO-1 MmfyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzHRY1bUUN3ME2zMlQ6OjB2IN88US=> MYHTRW5ITVJ?
HCT-116 M{HobGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPlcWo{UUN3ME2zMlUxPzFzIN88US=> Mn;PV2FPT0WU
CAL-120 NELW[4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTNwNUezOUDPxE1? MWDTRW5ITVJ?
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NCI-H524 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPveVFKSzVyPUG5MlU4OjVizszN MUjTRW5ITVJ?
KU812 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDqTWM2OD1zOT61PVQ1KM7:TR?= MWXTRW5ITVJ?
NB10 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M334VGlEPTB;MUmuOlI3QCEQvF2= MlnMV2FPT0WU
NOS-1 NUHV[G85T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vnTWlEPTB;MkCuNVU4PyEQvF2= NGftS4lUSU6JRWK=
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LB2518-MEL M4H2WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX36fGdLUUN3ME2yNE4{QTV|IN88US=> MW\TRW5ITVJ?
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NCI-H2122 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vZTmlEPTB;MkKuNVY4PyEQvF2= NV3SN25CW0GQR1XS
IMR-5 NVyzbJZPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3j[oRKSzVyPUKyMlI5PyEQvF2= NEHneYVUSU6JRWK=
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SW1573 NIfKTpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVS4WHlxUUN3ME2yN{4xPjR4IN88US=> MnTxV2FPT0WU
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SKG-IIIa MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPvZm1KSzVyPUKzMlM2PDZizszN Mm[0V2FPT0WU
MC-CAR M4nBR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTJ|LkWxPFMh|ryP NXnZW5h[W0GQR1XS
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EFM-19 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LnVmlEPTB;MkSuNVg1PCEQvF2= NGHnO|dUSU6JRWK=
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SW1990 NVzieJlDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mor0TWM2OD1{ND61PFg{KM7:TR?= MWfTRW5ITVJ?
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CCRF-CEM NGPk[4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;LcFZSUUN3ME2yOU44PDV7IN88US=> MVnTRW5ITVJ?
CAPAN-1 MkOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTJ3Lke1NFEh|ryP NWXEdZRCW0GQR1XS
CP50-MEL-B MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPCUoNkUUN3ME2yOU46QTBzIN88US=> M{fKWXNCVkeHUh?=
FADU NFTLOIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDzS5Q1UUN3ME2yOk42OTV{IN88US=> NUXyeGNnW0GQR1XS
Daudi M4nPUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTJ4LkWxOVgh|ryP Ml72V2FPT0WU
U-87-MG NILoXpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF31eJhKSzVyPUK2MlU{ODVizszN MoHGV2FPT0WU
DSH1 NET3eGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHO2PGRKSzVyPUK2MlU1ODZizszN M{T2UHNCVkeHUh?=
KYSE-520 NX7qbYhET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TNfWlEPTB;Mk[uOVU2OyEQvF2= Mk\QV2FPT0WU
HuH-7 NEPS[GtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTJ4LkmwPFYh|ryP MXHTRW5ITVJ?
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HDLM-2 NFW4U3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTJ6LkK0OVkh|ryP M4rjSXNCVkeHUh?=
NCI-H1651 M2jUXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUHrNHB3UUN3ME2yPE4zPTl{IN88US=> NFzSN4JUSU6JRWK=
SK-N-AS M4[xTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\2eHRJUUN3ME2yPE4{OTdzIN88US=> M3HsbXNCVkeHUh?=
NY M2jo[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\STWM2OD1{OD62OVc6KM7:TR?= MnHCV2FPT0WU
D-283MED M1HIWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jDWGlEPTB;MkiuPFcxQCEQvF2= NEnnR5JUSU6JRWK=
KLE NVzoUJVjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDuTFVsUUN3ME2yPE46QTB4IN88US=> NV\LSIx4W0GQR1XS
NCI-H28 M{DmXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nz[WlEPTB;MkmuNFEzKM7:TR?= M3PkeXNCVkeHUh?=
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CAL-54 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYPpeY1NUUN3ME2yPU43ODR4IN88US=> MYTTRW5ITVJ?
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OVCAR-8 Mk\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPmZnhUUUN3ME2zNE43Pzl2IN88US=> NYDYXGgzW0GQR1XS
NCI-H630 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHKboNKSzVyPUOwMlg6PTNizszN NFuzNmRUSU6JRWK=
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KYSE-70 Mnn3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\PcIVqUUN3ME2zNU4{ODB6IN88US=> MnPDV2FPT0WU
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NCI-H2228 M4rpbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\tVmlEPTB;M{GuOVYzPSEQvF2= M{D2cHNCVkeHUh?=
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DJM-1 M4HUN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1q3dmlEPTB;M{KuNVM5PiEQvF2= MWfTRW5ITVJ?
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LU-165 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\YTWM2OD1|Mz6wNFgh|ryP MljrV2FPT0WU
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NCI-H441 MlLSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2\TT2lEPTB;M{OuNlI{PSEQvF2= Mn;JV2FPT0WU
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MC-IXC M{\IUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M361RWlEPTB;M{OuOVY2PSEQvF2= M3ezdXNCVkeHUh?=
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SW1463 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTN4Lk[1OlYh|ryP MWnTRW5ITVJ?
KYSE-140 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnT5TWM2OD1|Nj63Nlk5KM7:TR?= M1zKVnNCVkeHUh?=
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Capan-2 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTFTW5KSzVyPUO5MlA2ODlizszN MnvHV2FPT0WU
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NCI-H2170 MnG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrKRlByUUN3ME2zPU46QTd5IN88US=> NEfWNplUSU6JRWK=
COLO-678 M4rnfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HhfmlEPTB;NECuOVk2KM7:TR?= MoixV2FPT0WU
PF-382 M{PNd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2fIfmlEPTB;NECuO|Q5PyEQvF2= MVHTRW5ITVJ?
OAW-42 NUXhZVh1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jaSmlEPTB;NEKuNlk1OiEQvF2= Ml75V2FPT0WU
LB996-RCC MmTnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTR{LkiwNFgh|ryP MWrTRW5ITVJ?
SAS M2PoUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXWZZdKSzVyPUSzMlQzQDhizszN NHfJbJVUSU6JRWK=
MRK-nu-1 NWTySVlXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\uUpZNUUN3ME20OE44PDF2IN88US=> MmXnV2FPT0WU
EoL-1-cell MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTR2LkmxN|Yh|ryP NFnUPGFUSU6JRWK=
NCI-H2342 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkPkTWM2OD12NT6xNVE4KM7:TR?= NF3JcWZUSU6JRWK=
NCI-H1703 NYKwbGJoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{[2PWlEPTB;NE[uNFU6OiEQvF2= NELjSGtUSU6JRWK=
NCI-H187 MkK4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\GTWM2OD12Nj6xPFY6KM7:TR?= NIW5SZVUSU6JRWK=
CP66-MEL MkntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzsS5l7UUN3ME20Ok4zPzh7IN88US=> MYXTRW5ITVJ?
HCC1569 MmrXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\WTW9KSzVyPUS2MlU6OTdizszN MWPTRW5ITVJ?
BL-70 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLUTWM2OD12Nj63O|M4KM7:TR?= M{L4fXNCVkeHUh?=
Saos-2 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUS5XZFrUUN3ME20O{4{OTJ|IN88US=> MnW4V2FPT0WU
LC-1F NInkZWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7kTWM2OD12Nz62NVA2KM7:TR?= M3HPOHNCVkeHUh?=
KY821 NF3yWllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXUe5dKSzVyPUS3MlY1OzRizszN MVfTRW5ITVJ?
PSN1 MniyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzNTWM2OD12OD6xOFAyKM7:TR?= MlLNV2FPT0WU
RT4 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjRNXRtUUN3ME20PE45Ozd5IN88US=> NU\KbmgzW0GQR1XS
LS-1034 M3\5bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV:3RVBbUUN3ME20PE45QTV2IN88US=> MlHSV2FPT0WU
NCI-H1395 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XQbGlEPTB;NEmuNFQ6OiEQvF2= MVHTRW5ITVJ?
EKVX M{\DO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml:xTWM2OD12OT62O|MyKM7:TR?= NFy3VWZUSU6JRWK=
IST-MES1 MoLjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYT1XZNTUUN3ME20PU45QTR2IN88US=> MYPTRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo PF-562271 (< 33 mg/kg p.o.) inhibits FAK phosphorylation in tumors in a dose- and time-dependent manner in U87MG-bearing mice. PF-562271 (50 mg/kg p.o. bid) results in 86% tumor growth inhibition in BxPc3 xenografts mice and 45% tumor growth inhibition in PC3-M xenografts mice. PF-562271 (25 mg/kg, bid) results in 2-fold greater apoptosis in treated tumors in mice bearing H125 lung xenografts. [1] PF-562271 (33 mg/kg, p.o.) inhibits extensive movement of the tumor cells over 24 hours in mice. PF-562271 (33 mg/kg, p.o.) results in altered E-cadherin dynamics in mice, which correlates with reduced E-cadherin–dependent collective cell movement. [3] PF-562271 (25 mg/kg, p.o. bid) results in 62% tumor growth inhibition in PC3M-luc-C6 subcutaneuous local implant xenograft mouse model. [4] PF-562,271 (5 mg/kg, oral) results in significant and similar increases in osteocalcin and cancellous bone parameters and a decrease in tumor growth and signs of bone healing in rats implanted with MDA-MB-231 cells in the tibia. [5]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Athymic female mice bearing BxPc3 or PC3-M xenografts.
  • Formulation: 5% Gelucire
  • Dosages: 50 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (197.04 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.49
Formula

C21H20F3N7O3S

CAS No. 717907-75-0
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

FAK Signaling Pathway Map

Related FAK Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID