Ouabain

Catalog No.S4016 Synonyms: NSC 25485

Ouabain Chemical Structure

Molecular Weight(MW): 728.77

Ouabain is a selective Na+/K+, -ATPase inhibitor, binds to α2 /α3 subunit with Ki of 41 nM/15 nM.

Size Price Stock Quantity  
In DMSO USD 220 In stock
USD 170 In stock
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1 Customer Review

  • Effect of pharmacological agents capable of increasing intracellular calcium concentration on RSV. HEp-2 cells were infected with RSV strain long at an MOI of 0.1, and treated with a specific Na+/K+-ATPase inhibitor ouabain at indicated concentrations. Supernatants were collected at 48 h postinfection and viral titers were determined by immunoplaque assay. Grey bars represent cytotoxicity of the compounds. The data presented were obtained from two independent experiments. Error bars represent the standard deviations from two independent experiments. Statistical significance between treated and control group were analyzed by t-test (*, p < 0.05, **, p < 0.01).

    Antiviral Res, 2016, 132:38-45.. Ouabain purchased from Selleck.

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Biological Activity

Description Ouabain is a selective Na+/K+, -ATPase inhibitor, binds to α2 /α3 subunit with Ki of 41 nM/15 nM.
Features A glycoside poison that binds to and inhibits the action of the Na+/K+ pump in the cell membrane.
Targets
α3 [1] α2 [1]
15 nM(Ki) 41 nM(Ki)
In vitro

Ouabain (100 nM) inhibits ATPase activities with 25%. [1] Ouabain (0.1 μM-1.0 μM) inhibits the Na+ pump and increases stored Ca2+ in cultured rat astrocytes. High Ouabain affinity isoforms (alpha2 in astrocytes, alpha3 in neurons and myocytes) are confined to a reticular distribution within the PM that paralleled underlying endoplasmic or sarcoplasmic reticulum. [2] Ouabain (0.5-1.0 mM) increases the levels of alpha1 and beta1 mRNAs, whereas it decreases those of alpha2 and beta2 mRNAs in cultured rat astrocytes. Ouabain increases alpha1 and beta1, but not alpha2 and beta2, proteins, and that the isoforms in control and ouabain-treated cultures. The ouabain-induced increase in alpha1 mRNA is blocked by cycloheximide (10 mM), the intracellular Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetraacetoxymethyl ester (30 mM), and FK506 (1 nM) in cultured rat astrocytes. [3] Ouabain (10 μM) induces significant PMD in both cell lines (28.1% of MDCK cells and 47.9% of Ma104 cells are gated in region M1, against 11.8% and 14.6% of the respective controls), but unexpectedly this effect is more remarkable in Ma104 cells. Ouabain (10 μM) induces a sustained increase in P-Tyr in MDCK cells and GSH almost completely reverted this effect, while the effect is not significant in Ma104 cells. [4]

In vivo Ouabain (14.4 mg/kg.d s.c. intermittent) further increases total peripheral resistance (TPR) in rats with heart failure due to myocardial infarction (MI), while continuous Ouabain treatment normalized TPR in rats. Ouabain (14.4 mg/kg.d s.c. continuous) significantly improves basal and maximal CO (basal: 83 mL/min; maximal: 134 mL/min). [5]

Protocol

Animal Research:[5]
+ Expand
  • Animal Models: Male Wistar rats with heart failure due to myocardial infarction (MI)
  • Formulation: Saline
  • Dosages: 14.4 mg/kg
  • Administration: Subcutaneous
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (137.21 mM)
Ethanol 100 mg/mL (137.21 mM)
Water slightly soluble or insoluble
In vivo

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 728.77
Formula

C29H44O12.8H2O

CAS No. 630-60-4
Storage powder
in solvent
Synonyms NSC 25485

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID