Name: Liraglutide
Brand: Selleck Chemicals
SKU: S8256
Availability: InStock
Rating: 5 (27 reviews)

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Quality Control

Batch: Purity: 99.84%

99.84

Related Targets	CXCR Hedgehog/Smoothened PKA Adrenergic Receptor AChR 5-HT Receptor Histamine Receptor Dopamine Receptor Ras KRas
Other GLP-1 Receptor Inhibitors	Danuglipron (PF-06882961) Exenatide Acetate (Exendin-4) Survodutide Ecnoglutide（XW003） AZD5004 (ECC5004) Brenipatide CT-996(RO7795081，RG6652） Ribupatide

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
HepG2	Proliferation assay	0, 1, 10, 100, and 1000 nmol/L	48 h	IC50~100 nM	31081108
MiaPaca-2	Cell viability assay	0, 10, 100, 1000 nM	48 h	The inhibitory effects on cell viability and cell number were statistically significant only at the concentration of 1,000 nmol/L for liraglutide	29897998
H9c2	Function assay	100 nM	1 h	Liraglutide reversed the downregulation of SIRT1 induced by TNF-α and hypoxia	29571736
MC3T3-E1	Apoptosis assay	0, 10, 100, or 1000 nM	48 h	liraglutide suppressed the apoptosis of MC3T3-E1 cells	29463067
MCF-7	Proliferation assay	10, 100, 1,000 and 10,000 nM	48 h	Liraglutide inhibits the proliferation and promotes apoptosis in the MCF-7 breast cancer cell line	29393459
SH-SY5Y	Function assay	100 nM	16 h	Liraglutide resolves UPR in the neuroblastoma SH-SY5Y cell line.	29170452
HEK293	Function assay		up to 30 mins	Agonist activity at human GLP1 receptor expressed in HEK293 cells harboring mCerulean and mCitrine fused Epac protein assessed as increase in cAMP level up to 30 mins by fluorescence assay, EC50 = 0.000067 μM.	28771355
HEK293	Function assay		30 mins	Agonist activity at human GLP1 receptor expressed in HEK293 cells assessed as increase in cAMP level after 30 mins by HTRF method, EC50 = 0.0074 μM.	ChEMBL
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (26.65 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 100 mg/mL

Ethanol : 100 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (1.33mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	3751.25	Formula	C₁₇₂H₂₆₅N₄₃O₅₁	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	204656-20-2	Download SDF Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NN2211, Liraglutida, Liraglutidum			SMILES	CCCCCCCCCCCCCCCC(=O)NC(CCC(=O)NCCCCC(C(=O)NC(CCC(=O)O)C(=O)NC(CC1=CC=CC=C1)C(=O)NC(C(C)CC)C(=O)NC(C)C(=O)NC(CC2=CNC3=CC=CC=C32)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CCCNC(=N)N)C(=O)NCC(=O)NC(CCCNC(=N)N)C(=O)NCC(=O)O)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CCC(=O)N)NC(=O)CNC(=O)C(CCC(=O)O)NC(=O)C(CC(C)C)NC(=O)C(CC4=CC=C(C=C4)O)NC(=O)C(CO)NC(=O)C(CO)NC(=O)C(C(C)C)NC(=O)C(CC(=O)O)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C(CC5=CC=CC=C5)NC(=O)C(C(C)O)NC(=O)CNC(=O)C(CCC(=O)O)NC(=O)C(C)NC(=O)C(CC6=CN=CN6)N)C(=O)O

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	GLP-1 receptor
In vitro	Liraglutide attenuates induction of plasminogen activator inhibitor type-1 (PAI-1) and vascular adhesion molecule (VAM) expression in human vascular endothelial cells (hVECs) in vitro and may afford protection against endothelial cell dysfunction (ECD), an early abnormality in diabetic vascular disease. In vitro studies demonstrates GLP-1R-dependent this compound-mediated inhibition of stimulated PAI-1 and VAM expression.
In vivo	In vivo studies of vascular reactivity and immunohistochemical analysis are performed in the ApoE-/- mouse model. They demonstrate significant improvement in endothelial function in liraglutide treated mice, a GLP-1R dependent effect. This compound treatment also increases endothelial nitric oxide synthase (eNOS) and reduces intercellular adhesion molecule-1 (ICAM-1) expression in aortic endothelium, an effect again dependent on the GLP-1R. It reduces hyperglycemia in T2D mouse models by increasing pancreatic b cell mass through enhanced proliferation.
References	[1] https://pubmed.ncbi.nlm.nih.gov/21517658/ [2] https://pubmed.ncbi.nlm.nih.gov/26876561/ [3] https://pubmed.ncbi.nlm.nih.gov/21562063/

Applications

Methods	Biomarkers	Images	PMID
Western blot	p-ERK / p-JNK / p-p38 / p-AKT / IκB-α		24217090

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2025-05-29)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06742710	RECRUITING	PCOS; Obesity; Infertility	Peking University Third Hospital	2025-05-22	PHASE4
NCT06976619	RECRUITING	Type 1 Diabetes; Type 2 Diabetes	Mayo Clinic	2025-10-03	PHASE2
NCT05681299	RECRUITING	Healthy; Growth Hormone Deficiency	Columbia University	2023-05-01	PHASE4
NCT07244003	RECRUITING	T2DM (Type 2 Diabetes Mellitus)	Hangzhou Zhongmei Huadong Pharmaceutical Co., Ltd.	2025-03-29	PHASE4
NCT07301437	NOT_YET_RECRUITING	Overweight and Obese Adults	The Fourth Affiliated Hospital of Zhejiang University School of Medicine	2026-05-01	PHASE4
NCT06171152	SUSPENDED	Multiple Sclerosis; Long COVID; Long Covid19; Obese; Obesity; Obesity, Morbid; Acute Leukemia in Remission	University of Chicago	2024-01-26	EARLY_PHASE1

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Liraglutide Glp-1 Receptor agonist

Chemical Structure

Molecular Weight: 3751.25