ID-8

Catalog No.S7327

ID-8 Chemical Structure

Molecular Weight(MW): 298.29

ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.

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USD 370 In stock

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Biological Activity

Description ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.
Targets
DYRK [1]
In vitro

ID-8 reversibly maintains the long-term culture of ESCs by sustaining Nanog gene expression through Sox2-Oct3/4 activation. [1] ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs. The mechanism study indicates that ID-8 maintains the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs. [2]

Protocol

Cell Research
+ Expand
  • Cell lines: Human embryonic stem cells
  • Concentrations: ~10 μM
  • Incubation Time: 7 days
  • Method: Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 60 mg/mL (201.14 mM)
Ethanol 2 mg/mL (6.7 mM)
Water <1 mg/mL
In vivo

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 298.29
Formula

C16H14N2O4

CAS No. 147591-46-6
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID