ID-8

Catalog No.S7327

ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.

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ID-8 Chemical Structure

ID-8 Chemical Structure
Molecular Weight: 298.29

Validation & Quality Control

Quality Control & MSDS

Product Information

Product Description

Biological Activity

Description ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.
Targets DYRK [1]
In vitro ID-8 reversibly maintains the long-term culture of ESCs by sustaining Nanog gene expression through Sox2-Oct3/4 activation. [1] ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs. The mechanism study indicates that ID-8 maintains the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs. [2]
In vivo
Features

Protocol(Only for Reference)

Cell Assay: [2]

Cell lines Human embryonic stem cells
Concentrations ~10 μM
Incubation Time 7 days
Method Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)0.020.151.80.40.0810
Body Surface Area (m2)0.0070.0250.150.050.020.5
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

References

[1] Miyabayashi T, et al. Biosci Biotechnol Biochem. 2008 , 72(5), 1242-1248.

[2] Hasegawa K, et al. Stem Cells Transl Med. 2012, 1(1), 18-28.

Chemical Information

Download ID-8 SDF
Molecular Weight (MW) 298.29
Formula

C16H14N2O4

CAS No. 147591-46-6
Storage 3 years -20℃powder
2 years -80℃in solvent
Synonyms N/A
Solubility (25°C) * In vitro DMSO 60 mg/mL (201.14 mM)
Ethanol 2 mg/mL (6.7 mM)
Water <1 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name 1-(4-Methoxyphenyl)-2-methyl-3-nitro-1H-indol-6-ol

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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