Catalog No.S7327

ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.

Price Stock Quantity  
USD 97 In stock
USD 370 In stock
Bulk Inquiry

Massive Discount Available

Free Overnight Delivery on all orders over $ 500.

ID-8 Chemical Structure

ID-8 Chemical Structure
Molecular Weight: 298.29

Validation & Quality Control

Quality Control & MSDS

Product Information

Product Description

Biological Activity

Description ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.
Targets DYRK [1]
In vitro ID-8 reversibly maintains the long-term culture of ESCs by sustaining Nanog gene expression through Sox2-Oct3/4 activation. [1] ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs. The mechanism study indicates that ID-8 maintains the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs. [2]
In vivo

Protocol(Only for Reference)

Cell Assay: [2]

Cell lines Human embryonic stem cells
Concentrations ~10 μM
Incubation Time 7 days
Method Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)
Body Surface Area (m2)0.0070.0250.
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)


[1] Miyabayashi T, et al. Biosci Biotechnol Biochem. 2008 , 72(5), 1242-1248.

[2] Hasegawa K, et al. Stem Cells Transl Med. 2012, 1(1), 18-28.

Chemical Information

Download ID-8 SDF
Molecular Weight (MW) 298.29


CAS No. 147591-46-6
Storage 3 years -20℃powder
2 years -80℃in solvent
Synonyms N/A
Solubility (25°C) * In vitro DMSO 60 mg/mL (201.14 mM)
Ethanol 2 mg/mL (6.7 mM)
Water <1 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name 1-(4-Methoxyphenyl)-2-methyl-3-nitro-1H-indol-6-ol

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

* Indicates a Required Field

Related Others Products

  • CB-839

    CB-839 is a potent, selective, and orally bioavailable glutaminase inhibitor with IC50 of 24 nM for recombinant human GAC. Phase 1.

  • GKT137831

    GKT137831 is a potent, dual NADPH oxidase NOX1/NOX4 inhibitor with Ki of 110 nM and 140 nM, respectively. Phase 2.

  • LB-100

    LB-100 is a water soluble protein phosphatase 2A (PP2A) inhibitor. Phase 1.

  • Puromycin 2HCl

    Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.

  • Cyclosporin A

    Cyclosporin A is an immunosuppressive agent, binds to the cyclophilin and then inhibits calcineurin with IC50 of 7 nM in a cell-free assay, widely used in organ transplantation to prevent rejection.

  • Cyclophosphamide Monohydrate

    Cyclophosphamide Monohydrate is a nitrogen mustard alkylating agent, it attaches the alkyl group to the guanine base of DNA.

  • Ganciclovir

    Ganciclovir is an antiviral drug for feline herpesvirus type-1 with IC50 of 5.2 μM in a cell-free assay.

  • Calcitriol

    Calcitriol is the hormonally active form of vitamin D.

  • Ribavirin

    Ribavirin, a synthetic guanosine analogue, possesses a broad spectrum of activity against DNA and RNA viruses.


    BAPTA-AM is a selective, membrane-permeable calcium chelator.

Recently Viewed Items

Tags: buy ID-8 | ID-8 supplier | purchase ID-8 | ID-8 cost | ID-8 manufacturer | order ID-8 | ID-8 distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Contact Us