Ganciclovir

Catalog No.S1878 Synonyms: RS-21592, BW-759

Ganciclovir Chemical Structure

Molecular Weight(MW): 255.23

Ganciclovir is an antiviral drug for feline herpesvirus type-1 with IC50 of 5.2 μM in a cell-free assay.

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 70 In stock
USD 270 In stock
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Biological Activity

Description Ganciclovir is an antiviral drug for feline herpesvirus type-1 with IC50 of 5.2 μM in a cell-free assay.
In vitro

Ganciclovir is metabolized to the triphosphate form by primarily three cellular enzymes: (1) a deoxyguanosine kinase induced by CMV-infected cells; (2) guanylate kinase; and (3) phosphoglycerate kinase. [1] Ganciclovir is sufficient to induce cell death in most bystander cells cocultured with HSV-tk-expressing cells. [2] Ganciclovir significantly reduces DNA synthesis in the transformed cells, whereas Ganciclovir has little effect on DNA synthesis in the nontransformed cells. Ganciclovir exhibits a concentration-dependent reduction in the rate of elongation into mature DNA. [3] Ganciclovir induces cell cycle arrests rather than direct chemical effect on HSVtk-transduced B16F10 melanoma cells. [4] Ganciclovir produces only weak inhibition of DNA synthesis. Ganciclovir-treated cells accumulate in early S-phase and remained there until cell death, suggesting that ganciclovir incorporation in the DNA template is important for cytotoxicity. [5] Ganciclovir-induced apoptosis is due to incorporation of the drug into DNA resulting in replication-dependent formation of DNA double-strand breaks and, at later stages, S and G2/M arrest. Ganciclovir-provoked DNA instability is likely to be responsible for the observed initial decline in Bcl-2 level and caspase-9/-3 activation. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
E6SM cell lines MmO5SpVv[3Srb36gZZN{[Xl? NHO4dJJG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JGhmenCnczDzbY1xdGW6II\pdpV{NTJiKFjTWk0zMSCrbnT1Z4VlKGO7dH;wZZRpcWOrdImgZpkhPTBnIHnuJGU3W01iY3XscEBtcW6nczygSWM2OD1zLkKgcm0> M4XEdFEyPDl3NUi2
human OST TK-cells MoToR5l1d3SxeHnjxsBie3OjeR?= MWDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDPV3QhXEtvY3XscJMh\XiycnXzd4lv\yCKU2[xJHRMNCCLQ{WwQVEvQSCwTR?= NULXOlNYOTdzOEGxOVg>
HEL cells Mmf2SpVv[3Srb36gZZN{[Xl? M{TscWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhUXjFiS1;TJIlv\mWldHXkJIlvKEiHTDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjpZ{Bm\m[nY4SsJGVEPTB;MUCgcm0> NInkWpUzOTJ|MkiyPC=>
HFF cells NWTac2Z5TnWwY4Tpc44h[XO|YYm= M1O0dVExKGSjeYO= NULoS4ROSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEOPVjDUc5dv\SCrbn\lZ5Rm\CCrbjDISmYh[2WubIOgbY5kfWKjdHXkJIZweiBzMDDkZZl{KGK7IIDsZZF2\SC{ZXT1Z5Rqd25iYYPzZZktKEmFNUC9NE4yPCEQvF2= MViyNVgyOjR{MB?=
MCA-TK cells Mn3pR5l1d3SxeHnjxsBie3OjeR?= MnzKR5l1d3SxeHnjbZR6KGGpYXnud5QhVUODLWTLJINmdGy|LDDJR|UxRTBwMUWg{txO M13ETlE4QTZyOUK2
HFF cells M121[WZ2dmO2aX;uJIF{e2G7 M2Lqd2lvcGmkaYTpc44hd2ZiSFPNWkBCTDF4OTDy[ZBtcWOjdHnvckBqdiCKRl[gZ4VtdHNiYomgZ5l1d3CjdHjpZ{Bm\m[nY4SgZZN{[XluIFXDOVA:OC5zNTFOwG0> M37WPFE4ODB2N{K2
Vero cells M1\BW2Z2dmO2aX;uJIF{e2G7 MnrjRY51cX[rcnHsJIFkfGm4aYT5JIRmfGW{bXnu[YQh[WejaX7zeEBp\XKyZYOgd4lueGyneDD0fZBmKDFiKF[gd5Rz[WmwKTDifUBxdGGzdXWgdoVlfWO2aX;uJIlvKF[ncn:gZ4VtdHNuIFnEOVA:OC5{IN88US=> NHnmOGQ{ODF4Mk[z
human HS27 cells NXv0T3dvTnWwY4Tpc44h[XO|YYm= NUThcHQ3PyCmYYnz NWjpPWpVSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gZ5l1d22nZ3Hsc5ZqenW|IHnu[oVkfGWmIHnuJIh2dWGwIFjTNlch[2WubIOgZYZ1\XJiNzDkZZl{KGK7IFfGVE1j[XOnZDDmcJVwemW|Y3XueEBz\WS3Y4Tpc44h[XO|YYmsJGVEPTB;MD6zNkDPxE1? MnXqNlAxPDd7MUG=
MRC5 cells MYjGeY5kfGmxbjDhd5NigQ>? MV7BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIR21XKGmwIF3SR|Uh[2WubIOgZpkheGyjcYXlJJJm\HWldHnvckBie3OjeTygTWM2OD1yLkmxJO69VQ>? NXnuU4JJOTd{M{m1PVQ>
BSC-1 cells M2LYVGZ2dmO2aX;uJIF{e2G7 NYTZXWF6SW62aY\pdoFtKGGldHn2bZR6KG:oIITo[UBkd22yb4Xu[EB4[XNiZY\hcJVifGWmIHHnZYlve3RidHjlJGhmenCnczDzbY1xdGW6II\pdpV{KHS7cHWtNUBqdiCEU1OtNUBk\WyuczygTWM2OD1|IN88US=> MoXlNlkyOzNyMB?=
MEF cells MoSzSpVv[3Srb36gZZN{[Xl? MknvTY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RibYXybY5mKGO7dH;t[YdidG:4aYL1d{Bz\XCuaXPheIlwdiCrbjDNSWYh[2WubIOge4F{KGSndHXycYlv\WRiYomgdIxieXWnIILl[JVkfGmxbjDhd5NigSxiSVO1NF0{NjRizszN NWXXeJh[QTR|OECxOy=>
CEM cells M{HkSGN6fG:2b4jpZ:Kh[XO|YYm= M3HUSWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEOHTTDj[YxteyxiQ1O1NF02KM7:TR?= M2r0elE2PjF3NUS1
mouse NIH 3T3 cells NHfrW49HfW6ldHnvckBie3OjeR?= NWjsWJVJPC13IHThfZM> M172d2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JG12emmwZTDjfZRwdWWpYXzveolzfXNic4TyZYlvKFOvaYToJIlv\mWldHXkJIlvKG2xdYPlJG5KUCB|VEOgZ4VtdHNiYX\0[ZIhPCC2bzC1JIRigXNiYomgdIxieXWnIILl[JVkfGmxbjDhd5NigSxiRVO1NF02NjdizszN MUixPFQ2QDF{NB?=
RG2TK+ cells NWnJSZJkS3m2b4TvfIlkyqCjc4PhfS=> Ml7nO|IhcA>? NUj5Tm44S3m2b4TvfIlkcXS7IHHnZYlve3RiSGPWNU11cyCpZX7lJI93\XKneIDy[ZN{cW6pIGLHNnRMMyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBESzVyPUWuPFYh|ryP MoHWNVg5ODB5NkS=
human bone marrow cells MoTVR5l1d3SxeHnjxsBie3OjeR?= MXGxOUBl[Xm| MYjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDic45mKG2jcoLve{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFPGWU1IVSCob4LtZZRqd25iYX\0[ZIhOTViZHH5d{whS0N3ME2zNEDPxE1? Ml[0NVc{OjlzMEO=

... Click to View More Cell Line Experimental Data

In vivo Antiviral drug ganciclovir (GCV) inhibits the proliferation of microglia in experimental autoimmune encephalomyelitis (EAE). GCV attenuates neuroinflammation and does not significantly restrain the peripheral immune response[7].

Protocol

Cell Research:

[7]

+ Expand
  • Cell lines: BV-2 cells 
  • Concentrations: --
  • Incubation Time: 24 h
  • Method:

    To assess cell proliferation by thymidine incorporation, a total of 2 × 10<sup>4</sup> BV-2 cells were seeded in a 96-well plate for a maximum of 12 h in 10% FBS containing DMEM and then serum starved for an additional 12 h before the addition of varying concentrations of GCV for a total of 24 h. Cultures were pulsed for the final 8 h with 1 µCi/well [3H]thymidine before incorporated radioactivity was measured using a β plate scintillation counter.


    (Only for Reference)
Animal Research:

[7]

+ Expand
  • Animal Models: C57BL/6 mice
  • Formulation: PBS
  • Dosages: 100 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 27 mg/mL warmed (105.78 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
4% DMSO+30% PEG 300+ddH2O
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 255.23
Formula

C9H13N5O4

CAS No. 82410-32-0
Storage powder
Synonyms RS-21592, BW-759

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00034385 Completed Kidney Trasplant National Institutes of Health Clinical Center (CC) April 24, 2002 Phase 4
NCT00001328 Completed Brain Neoplasm|Neoplasm Metastasis National Institute of Neurological Disorders and Stroke (NINDS)|National Institutes of Health Clinical Center (CC) August 21, 1992 Phase 1
NCT02927067 Not yet recruiting Cytomegalovirus (CMV) Shire March 2017 Phase 3
NCT03004261 Recruiting Cytomegalovirus Infections|Hematological Disease Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine November 2016 Phase 4
NCT02606266 Not yet recruiting Congenital Cytomegalovirus (CMV) Assistance Publique - Hôpitaux de Paris October 2016 Phase 2|Phase 3
NCT02871401 Not yet recruiting Idiopathic Pulmonary Fibrosis Vanderbilt University|Genentech, Inc. September 2016 Phase 1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID