Ganciclovir

Catalog No.S1878 Synonyms: RS-21592, BW-759

Ganciclovir Chemical Structure

Molecular Weight(MW): 255.23

Ganciclovir is an antiviral drug for feline herpesvirus type-1 with IC50 of 5.2 μM in a cell-free assay.

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In DMSO USD 90 In stock
USD 70 In stock
USD 270 In stock
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  • Effect of ganciclovir on viral DNA accumulation (A,B) and induction of TGF-β1 production after human cytomegalovirus infection in human trabecular meshwork cells (C). Cells were harvested at 5 day post-infection at a high multiplicity of infection (MOI 1) under the treatment with different concentrations of ganciclovir (GAN). Treatment with 10 μmol of ganciclovir significantly decreased the viral DNA accumulation (p < 0.001) (A,B). However, treatment with ganciclovir did not affect the TGF-β production using a TGF-β1 luciferase bioassay. Results are expressed as the mean +/− standard deviation of three different experiments.

    Sci Rep, 2017, 7: 43349. Ganciclovir purchased from Selleck.

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Biological Activity

Description Ganciclovir is an antiviral drug for feline herpesvirus type-1 with IC50 of 5.2 μM in a cell-free assay.
In vitro

Ganciclovir is metabolized to the triphosphate form by primarily three cellular enzymes: (1) a deoxyguanosine kinase induced by CMV-infected cells; (2) guanylate kinase; and (3) phosphoglycerate kinase. [1] Ganciclovir is sufficient to induce cell death in most bystander cells cocultured with HSV-tk-expressing cells. [2] Ganciclovir significantly reduces DNA synthesis in the transformed cells, whereas Ganciclovir has little effect on DNA synthesis in the nontransformed cells. Ganciclovir exhibits a concentration-dependent reduction in the rate of elongation into mature DNA. [3] Ganciclovir induces cell cycle arrests rather than direct chemical effect on HSVtk-transduced B16F10 melanoma cells. [4] Ganciclovir produces only weak inhibition of DNA synthesis. Ganciclovir-treated cells accumulate in early S-phase and remained there until cell death, suggesting that ganciclovir incorporation in the DNA template is important for cytotoxicity. [5] Ganciclovir-induced apoptosis is due to incorporation of the drug into DNA resulting in replication-dependent formation of DNA double-strand breaks and, at later stages, S and G2/M arrest. Ganciclovir-provoked DNA instability is likely to be responsible for the observed initial decline in Bcl-2 level and caspase-9/-3 activation. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
E6SM cell lines NYr6c5M1TnWwY4Tpc44h[XO|YYm= NInwXFlG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JGhmenCnczDzbY1xdGW6II\pdpV{NTJiKFjTWk0zMSCrbnT1Z4VlKGO7dH;wZZRpcWOrdImgZpkhPTBnIHnuJGU3W01iY3XscEBtcW6nczygSWM2OD1zLkKgcm0> M2\YbVEyPDl3NUi2
human OST TK-cells NWHqeY5zS3m2b4TvfIlkyqCjc4PhfS=> M2CxbWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG9UXCCWSz3j[YxteyCneIDy[ZN{cW6pIFjTWlEhXEtuIFnDOVA:OS57IH7N MnPzNVcyQDFzNUi=
HEL cells NGfZVHFHfW6ldHnvckBie3OjeR?= MV;BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIV3YyKEuRUzDpcoZm[3SnZDDpckBJTUxiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXK3cz3pcoR2[2WmIHP5eI9x[XSqaXOg[YZn\WO2LDDFR|UxRTFyIH7N MX6yNVI{Ojh{OB?=
HFF cells NUOyd3ZDTnWwY4Tpc44h[XO|YYm= NFHZVGQyOCCmYYnz MkPNRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGNOXiCWb4fu[UBqdm[nY4Tl[EBqdiCKRl[gZ4VtdHNiaX7jeYJifGWmIH\vdkAyOCCmYYnzJIJ6KHCuYYH1[UBz\WS3Y4Tpc44h[XO|YYmsJGlEPTB;MD6xOEDPxE1? MkTjNlE5OTJ2MkC=
MCA-TK cells NFvqVIdEgXSxdH;4bYPDqGG|c3H5 MVTDfZRwfG:6aXPpeJkh[WejaX7zeEBOS0FvVFugZ4VtdHNuIFnDOVA:OC5zNTFOwG0> MkfiNVc6PjB7Mk[=
HFF cells NHnYRm5HfW6ldHnvckBie3OjeR?= M3z6TmlvcGmkaYTpc44hd2ZiSFPNWkBCTDF4OTDy[ZBtcWOjdHnvckBqdiCKRl[gZ4VtdHNiYomgZ5l1d3CjdHjpZ{Bm\m[nY4SgZZN{[XluIFXDOVA:OC5zNTFOwG0> NFW2SXYyPzByNEeyOi=>
Vero cells MX7GeY5kfGmxbjDhd5NigQ>? M2rWS2FvfGm4aYLhcEBi[3Srdnn0fUBl\XSncn3pcoVlKGGpYXnud5QhcGW{cHXzJJNqdXCuZYigeJlx\SBzIDjGJJN1emGrbjmgZpkheGyjcYXlJJJm\HWldHnvckBqdiCYZYLvJINmdGy|LDDJSFUxRTBwMjFOwG0> M3XF[FMxOTZ{NkO=
human HS27 cells MWnGeY5kfGmxbjDhd5NigQ>? MnzLO{Bl[Xm| MlPtRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iY4n0c41m\2Gub4\pdpV{KGmwZnXjeIVlKGmwIHj1cYFvKEiVMkegZ4VtdHNiYX\0[ZIhPyCmYYnzJIJ6KEeIUD3iZZNm\CCobIXvdoV{[2WwdDDy[YR2[3Srb36gZZN{[XluIFXDOVA:OC5|MjFOwG0> NXTLNVUyOjByNEe5NVE>
MRC5 cells NX[wd|hQTnWwY4Tpc44h[XO|YYm= NIrqVGFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJS02YIHnuJG1TSzViY3XscJMh[nlicHzhdZVmKHKnZIXjeIlwdiCjc4PhfUwhUUN3ME2wMlkyKM7:TR?= NWewdnF1OTd{M{m1PVQ>
BSC-1 cells M4[z[GZ2dmO2aX;uJIF{e2G7 NGLaWohCdnSrdnnyZYwh[WO2aY\peJkhd2ZidHjlJINwdXCxdX7kJJdieyCndnHseYF1\WRiYXfhbY5{fCC2aHWgTIVzeGW|IIPpcZBt\XhidnnyeZMhfHmyZT2xJIlvKEKVQz2xJINmdGy|LDDJR|UxRTNizszN M{HTcFI6OTN|MEC=
MEF cells Mo\nSpVv[3Srb36gZZN{[Xl? M2nuNmlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IH31dolv\SCleYTvcYVo[WyxdnnyeZMhemWybHnjZZRqd25iaX6gUWVHKGOnbHzzJJdieyCmZYTldo1qdmWmIHL5JJBt[XG3ZTDy[YR2[3Srb36gZZN{[XluIFnDOVA:Oy52IN88US=> M2HXeFk1OzhyMUe=
CEM cells MYLDfZRwfG:6aXRCpIF{e2G7 NEDUZYNEgXSxdH;4bYNqfHliYXfhbY5{fCCFRV2gZ4VtdHNuIFPDOVA:PSEQvF2= MlnINVU3OTV3NEW=
mouse NIH 3T3 cells NYXrTI1PTnWwY4Tpc44h[XO|YYm= M1O0ZlQuPSCmYYnz NIXX[25CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBOfXKrbnWgZ5l1d22nZ3Hsc5ZqenW|IIP0doFqdiCVbXn0bEBqdm[nY4Tl[EBqdiCvb4Xz[UBPUUhiM2SzJINmdGy|IHHmeIVzKDRidH:gOUBl[Xm|IHL5JJBt[XG3ZTDy[YR2[3Srb36gZZN{[XluIFXDOVA:PS55IN88US=> NGXLOYgyQDR3OEGyOC=>
RG2TK+ cells NY\BRpVtS3m2b4TvfIlkyqCjc4PhfS=> M1z0R|czKGh? MlziR5l1d3SxeHnjbZR6KGGpYXnud5QhUFOYMT30b{Bo\W6nIH;2[ZJmgHC{ZYPzbY5oKFKJMmTLL{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDDR|UxRTVwOE[g{txO NWjLfmRWOTh6MEC3OlQ>
human bone marrow cells M4PJVWN6fG:2b4jpZ:Kh[XO|YYm= M3;PSVE2KGSjeYO= MnLOR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gZo9v\SCvYYLyc5ch[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDDSnUuT01iZn;ycYF1cW:wIHHmeIVzKDF3IHThfZMtKEOFNUC9N|Ah|ryP NWXz[JVrOTd|MkmxNFM>

... Click to View More Cell Line Experimental Data

In vivo Antiviral drug ganciclovir (GCV) inhibits the proliferation of microglia in experimental autoimmune encephalomyelitis (EAE). GCV attenuates neuroinflammation and does not significantly restrain the peripheral immune response[7].

Protocol

Cell Research:

[7]

+ Expand
  • Cell lines: BV-2 cells 
  • Concentrations: --
  • Incubation Time: 24 h
  • Method:

    To assess cell proliferation by thymidine incorporation, a total of 2 × 10<sup>4</sup> BV-2 cells were seeded in a 96-well plate for a maximum of 12 h in 10% FBS containing DMEM and then serum starved for an additional 12 h before the addition of varying concentrations of GCV for a total of 24 h. Cultures were pulsed for the final 8 h with 1 µCi/well [3H]thymidine before incorporated radioactivity was measured using a β plate scintillation counter.


    (Only for Reference)
Animal Research:

[7]

+ Expand
  • Animal Models: C57BL/6 mice
  • Formulation: PBS
  • Dosages: 100 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 27 mg/mL warmed (105.78 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 255.23
Formula

C9H13N5O4

CAS No. 82410-32-0
Storage powder
Synonyms RS-21592, BW-759

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00034385 Completed Kidney Trasplant National Institutes of Health Clinical Center (CC) April 24, 2002 Phase 4
NCT00001328 Completed Brain Neoplasm|Neoplasm Metastasis National Institute of Neurological Disorders and Stroke (NINDS)|National Institutes of Health Clinical Center (CC) August 21, 1992 Phase 1
NCT02927067 Not yet recruiting Cytomegalovirus (CMV) Shire March 2017 Phase 3
NCT03004261 Recruiting Cytomegalovirus Infections|Hematological Disease Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine November 2016 Phase 4
NCT02606266 Not yet recruiting Congenital Cytomegalovirus (CMV) Assistance Publique - Hôpitaux de Paris October 2016 Phase 2|Phase 3
NCT02871401 Not yet recruiting Idiopathic Pulmonary Fibrosis Vanderbilt University|Genentech, Inc. September 2016 Phase 1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID