AMI-1

Catalog No.S7884

AMI-1 Chemical Structure

Molecular Weight(MW): 548.45

AMI-1 is a potent and specific Histone Methyltransferase (HMT) inhibitor with IC50 of 3.0 μM and 8.8 μM for yeast Hmt1p and human PRMT1, respectively.

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1 Customer Review

  • Effects of microinjection of AMI-1 into the RVLM on cardiovascular activity in the Ang II-induced hypertensive rats. The representative original tracings (A)

    Nitric Oxide, 2017, 1-10. AMI-1 purchased from Selleck.

Purity & Quality Control

Choose Selective Histone Methyltransferase Inhibitors

Biological Activity

Description AMI-1 is a potent and specific Histone Methyltransferase (HMT) inhibitor with IC50 of 3.0 μM and 8.8 μM for yeast Hmt1p and human PRMT1, respectively.
Targets
yeast Hmt1p [1]
(Cell-free assay)
human PRMT1 [1]
(Cell-free assay)
3.0 μM 8.8 μM
In vitro

In HeLa cells, AMI-1 inhibits methylation levels of GFP-Npl3 fusion and endogenous PRMT1-like activity. AMI-1 also inhibits nuclear receptor-mediated transactivation of a luciferase reporter in MCF7 cells. [1] In addition, AMI-1 inhibits HIV-1 RT polymerase activity with IC50 of 5 μM and inhibits DNA binding to HIV-1 RT with Kd of 2 μM. [2] In INS-1 cells, AMI-1 improves INS-1 cell function and mediates translocations of FOXO1 and PDX-1 intracellularly by regulating FOXO1 phosphorylation and methylation. [4]

In vivo In chronic AIPI rats, AMI-1 (5 μg/rat) ameliorates COX2 expression and asthmatic indexes, and decreases the airway and alveoli lesions, mucus secretion, and collagen deposition in lungs. [3]

Protocol

Animal Research:[3]
+ Expand
  • Animal Models: Chronic AIPI rats
  • Formulation: PBS
  • Dosages: 50 μL at a concentration of 0.1 mg/mL
  • Administration: Intranasal injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (182.33 mM)
Water 10 mg/mL (18.23 mM)
Ethanol Insoluble
In vivo Add solvents individually and in order:
PBS
22mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 548.45
Formula

C21H14N2Na2O9S2

CAS No. 20324-87-2
Storage powder
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Related Antibodies

Histone Methyltransferase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID