Home Metabolism SCD inhibitor PluriSIn #1 (NSC 14613) For research use only.

PluriSIn #1 (NSC 14613)

PluriSIn #1 is an inhibitor of the stearoyl-coA desaturase 1 (SCD1), which is able to selectively eliminate hPSCs.

PluriSIn #1 (NSC 14613) Chemical Structure

PluriSIn #1 (NSC 14613) Chemical Structure

CAS: 91396-88-2

Selleck's PluriSIn #1 (NSC 14613) has been cited by 2 publications

Purity & Quality Control

Batch: S807601 DMSO] 43 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.19%
99.19

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Biological Activity

Description PluriSIn #1 is an inhibitor of the stearoyl-coA desaturase 1 (SCD1), which is able to selectively eliminate hPSCs.
Targets
SCD1 [1]
In vitro
In vitro PluriSIns #1 has a robust, rapid, and selective cytotoxic effect toward hPSCs. Apoptosis is the central cell death mechanism activated by PluriSIn #1. PluriSIn #1 leads to ER stress in hPSCs. PluriSIn #1 (20 μM) induces ~30% decrease in protein synthesis in hPSCs. PluriSIn #1 exposure induces a ~65% decrease in stearoyl-coA desaturase (SCD1) activity in hPSCs. PluriSIn #1 (20 μM) prevents teratoma gormation from undifferentiated hPSCs PluriSIns also inhibits mPSCs and hinders mouse embryonic development. [1]
Kinase Assay SCD1 activity assays
Cells are plated in 6-well plates at a density of 50k to 100k cells per well. 24 h later, 20 μM PluriSIn #1 or 0.2% DMSO-control are added to the cells. After 12 h of incubation at 37 ℃, 5% CO2, the old medium is removed, cells are washed with PBS, and new medium containing 2.3 μM of 0.75 UCi [1-14C] Stearic Acid is added. The cells are incubated for up to 4 h at 37 ℃, 5% CO2. After the incubation period, the medium is discarded and the cells are washed 3 times with 2 mL of PBS. 2 mL of the mixture n-hexane: isopropanol (3:2 v:v) are added, and the cells are incubated for 30 min at 37 ℃, 5% CO2. 2 mL Folch solution (chloroform: methanol,2:1,v:v) are subsequently added. The liquid is transferred to tubes for phase partition by adding 1 mL water. The lower organic phase is evaporated and used for lipid saponification and TLC separation of the free [1-14C] Stearic Acid (substrate) and [1-14C] Oleic Acid (formed product). Lipids extracted from the cells are applied to TLC plates previously immersed in 10% NO3 Ag and activated at 120℃x60 min. Unlabeled stearic and oleic acid are added to each application point as carriers and as internal standards for identification. The plates are run with a solvent mixture of Chloroform:MeOH:AcH:DDW (90:8:1:0.8). The free fatty acids are detected by U.V. after spraying the TLC with a 2',7',dichlorofluorescein solution. The spots corresponding to stearic and oleic acid are scraped and the radioactivity counted in a sc intillating counter. SCD1 desaturase activity is calculated from the percent conversion of substrate to product and the conversion to pmol/min/106 cells.
Cell Research Cell lines Human iPSC line BJ-iPS28
Concentrations ~20 μM
Incubation Time 1 days
Method Relative cell numbers are determined by fixating the cells with 0.5% glutardialdehyde and staining with methylene blue dissolved in 0.1 M boric acid (pH 8.5). Color extraction is performed using 0.1 M hydrochloric acid, and the staining (which is proportional to cell number) is quantitated by measuring absorbance at 650 nM.

Chemical Information & Solubility

Molecular Weight 213.24 Formula

C12H11N3O
CAS No. 91396-88-2 SDF Download PluriSIn #1 (NSC 14613) SDF
Smiles C1=CC=C(C=C1)NNC(=O)C2=CC=NC=C2
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 43 mg/mL ( (201.65 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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