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PluriSIn #1 (NSC 14613) SCD inhibitor

Name: PluriSIn #1 (NSC 14613)
Brand: Selleck Chemicals
SKU: S8076
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S8076

PluriSIn #1 (NSC 14613) is an inhibitor of the stearoyl-coA desaturase 1 (SCD1) and is able to selectively eliminate hPSCs.

Chemical Structure

Molecular Weight: 213.24

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Quality Control

Batch: S807601 DMSO]43 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.19%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS
Datasheet

99.19

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other SCD Inhibitors	MK-8245 A939572 MF-438 Aramchol CAY10566 CVT-11127

Solubility

In vitro
Batch:

DMSO : 43 mg/mL (201.65 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.15mg/ml (10.08mM)	Taking the 1 mL working solution as an example, add 50 μL of 43 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.31mg/ml (1.45mM)	Taking the 1 mL working solution as an example, add 50 μL of 6.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	213.24	Formula	C₁₂H₁₁N₃O	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	91396-88-2	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1=CC=C(C=C1)NNC(=O)C2=CC=NC=C2

Mechanism of Action

Targets/IC₅₀/Ki	SCD1
In vitro	PluriSIn #1 (NSC 14613) has a robust, rapid, and selective cytotoxic effect toward hPSCs, with apoptosis as the central cell death mechanism it activates. This compound leads to ER stress in hPSCs, inducing a ~30% decrease in protein synthesis at 20 μM. It also causes a ~65% decrease in stearoyl-coA desaturase (SCD1) activity and prevents teratoma formation from undifferentiated hPSCs. PluriSIns also inhibits mPSCs and hinders mouse embryonic development.
Kinase Assay	SCD1 activity assays
Kinase Assay	Cells are plated in 6-well plates at a density of 50k to 100k cells per well. 24 h later, 20 μM PluriSIn #1 (NSC 14613) or 0.2% DMSO-control are added to the cells. After 12 h of incubation at 37 ℃, 5% CO₂, the old medium is removed, cells are washed with PBS, and new medium containing 2.3 μM of 0.75 UCi [1-¹⁴C] Stearic Acid is added. The cells are incubated for up to 4 h at 37 ℃, 5% CO₂. After the incubation period, the medium is discarded and the cells are washed 3 times with 2 mL of PBS. 2 mL of the mixture n-hexane: isopropanol (3:2 v:v) are added, and the cells are incubated for 30 min at 37 ℃, 5% CO₂. 2 mL Folch solution (chloroform: methanol,2:1,v:v) are subsequently added. The liquid is transferred to tubes for phase partition by adding 1 mL water. The lower organic phase is evaporated and used for lipid saponification and TLC separation of the free [1-¹⁴C] Stearic Acid (substrate) and [1-¹⁴C] Oleic Acid (formed product). Lipids extracted from the cells are applied to TLC plates previously immersed in 10% NO₃ Ag and activated at 120℃x60 min. Unlabeled stearic and oleic acid are added to each application point as carriers and as internal standards for identification. The plates are run with a solvent mixture of Chloroform:MeOH:AcH:DDW (90:8:1:0.8). The free fatty acids are detected by U.V. after spraying the TLC with a 2',7',dichlorofluorescein solution. The spots corresponding to stearic and oleic acid are scraped and the radioactivity counted in a scintillating counter. SCD1 desaturase activity is calculated from the percent conversion of substrate to product and the conversion to pmol/min/10⁶ cells.
References	[1] https://pubmed.ncbi.nlm.nih.gov/23318055/

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