Catalog No.S1199 Synonyms: 2-CdA, 2-chlorodeoxyadenosine
Molecular Weight(MW): 285.69
Cladribine is an adenosine deaminase inhibitor for U266, RPMI8226, and MM1.S cells with IC50 of approximately 2.43 μM, 0.75 μM, and 0.18 μM, respectively.
Cited by 1 publication
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|Description||Cladribine is an adenosine deaminase inhibitor for U266, RPMI8226, and MM1.S cells with IC50 of approximately 2.43 μM, 0.75 μM, and 0.18 μM, respectively.|
|Features||Cladribine is primarily active in lymphoid tissues.|
Cladribine exerts remarkable activity in hairy cell leukemia (HCL), a chronic B-cell lymphoproliferative disorder, producing prolonged complete remissions. Cladribine induces accumulation of DNA strand breaks, and subsequently activates the tumor suppressor p53 in lymphocytes. Cladribine may modulate STAT3 activity in MM cells. Cladribine inhibits proliferation/survival of U266, RPMI8226 and MM1.S cells in a dose-dependent manner. While U266 is the least sensitive cell line, MM1.S is the most sensitive one to cladribine. Treatment with cladribine gradually increases the percentage of cells in the G1 phase of the cell cycle and reduces the percentage of cells in S phase. Cladribine appears to increase G2-M phase in U266 cells upon 24 hour-treatment. A dose-dependent increase in apoptosis induced by cladribine is seen in both RPMI8226 and MM1.S cells. Treatment with cladribine at 0.2 μM dramatically induces activation of caspase-3, -8, and -9 and PARP cleavage in a time-dependent manner in MM1.S. Cladribine significantly decreases the phospho-STAT3 (P-STAT3) levels in a dose-dependent manner, but has no effect on the total STAT3 protein levels.  Cladribine possesses concentration-dependent apoptosis-inducing potential in the HSB2 cells.  Cladribine inhibits growth of primary mast cell (MC) and the MC line HMC-1 in a dose-dependent manner, with lower IC50 values recorded in HMC-1.2 cells harboring KIT D816V compared to HMC-1.1 cells lacking KIT D816V.  Cladribine decreases the migratory capacity of CD14+ monocytes, as well as of CD4+ and CD8+ T lymphocytes. 
|In vivo||Cladribine (0.7-3.5 mM) and/or diltiazem (2.4 mM), is injected intraperitoneally into adult zebrafish and red blood cell (RBC) lysates are assayed by HPLC for levels of purine nucleotides (e.g. ATP), potential biomarkers of cardiovascular health. Diltiazem increased RBC ATP concentrations, which are inhibited by co-injection of cladribine.  Plasma concentrations of Cladribine decreases rapidly following a biphasic decline after both ia and s.c. administrations. The AUC and t 1/2 beta after a single 1 mg/kg ia and 2 mg/kg s.c. injection of Cladribine are 0.66 vs 1.2 μg × h/mL and 3.5 vs 4.5 hours, respectively. |
-  Ma J, et al. BMC Cancer. 2011, 11, 255.
-  Guchelaar HJ, et al. Cancer Chemother Pharmacol. 1998, 42(1), 77-83.
-  B?hm A, et al. Exp Hematol. 2010, 38(9), 744-755.
|In vitro||DMSO||57 mg/mL (199.51 mM)|
|In vivo||Add solvents individually and in order:
5% DMSO+30% PEG 300+1% Tween 80+H2O
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Clinical Trial Information
|NCT Number||Recruitment||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT00923013||Recruiting||Hairy Cell Leukemia||National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC)||October 3, 2008||Phase 2|
|NCT02250937||Recruiting||Acute Myeloid Leukemia|Myelodysplastic Syndrome||M.D. Anderson Cancer Center||October 27, 2014||Phase 2|
|NCT02728050||Recruiting||Acute Biphenotypic Leukemia|Acute Myeloid Leukemia|de Novo Myelodysplastic Syndrome|Myeloproliferative Neoplasm||University of Washington|National Cancer Institute (NCI)||December 2016||Phase 1|Phase 2|
|NCT03012672||Recruiting||Acute Myeloid Leukemia|Myeloid Neoplasm||Fred Hutchinson Cancer Research Center|National Cancer Institute (NCI)||December 2016||--|
|NCT02921061||Recruiting||de Novo Myelodysplastic Syndrome|Mixed Phenotype Acute Leukemia|Previously Treated Myelodysplastic Syndrome|Recurrent Adult Acute Myeloid Leukemia|Untreated Adult Acute Myeloid Leukemia||Fred Hutchinson Cancer Research Center|National Cancer Institute (NCI)||November 2016||Phase 1|
|NCT02756572||Recruiting||Myelodysplastic Syndrome|Recurrent Adult Acute Myeloid Leukemia|Recurrent Childhood Acute Myeloid Leukemia||University of Washington|National Cancer Institute (NCI)||September 2016||--|
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