A unique collection of 4 specific signaling pathway inhibitors: PD0325901, an ERK/MEK inhibitor; CHIR99021, a GSK3 inhibitor; Thiazovivin, a Rho/ROCK inhibitor; SB431542, a TGFβ/ALK inhibitor. This stem cell enhancer kit can be used as a media additive to make reprogramming and expansion of human iPSCs (induced pluripotent stem cells) more efficient.
Human induced pluripotent stem cells (human iPSCs, hiPSCs), a Nobel prize-worthy discovery, hold enormous potential. They offer great promise for research and clinical applications, such as the modeling of human disease, screening of drug efficacy and safety, and ultimately serve as a source of autologous or allogeneic cells for regenerative medicine. Although rapid advances and adoption of this technology have already been accomplished, human iPSC generation is still a very slow (~4 weeks) and inefficient (≤ 0.01%) process. The slow kinetics and low efficiency of current human iPSC reprogramming and culture methods have been obstacles to impede their utility in biomedical research and clinical applications.
As future applications of human iPSC technology, genetic alteration for disease correction, loci-specific modulation for reporter systems and clone selection for preferred differentiation potential will require higher throughput and more reliable methods for clone derivation and characterization. Consequently, there is still a tremendous need for a safer, easier and more efficient procedure for human iPSC generation, which would also facilitate identifying and characterizing fundamental mechanisms of reprogramming.
To address some of the challenges of deriving and expanding human iPSCs, Selleck Chemicals launches a novel tool, Human iPSC Enhancer Kit. It is a mixture composed of 4 small molecule inhibitors, including PD0325901, CHIR99021, Thiazovivin, and SB431542, inhibiting ERK/MEK, GSK3, Rho/ROCK, and TGFβ/ALK pathway, respectively. The combination of these 4 stem cells enhancers has been shown to effectively enhance reprogramming and survival of human iPSCs.
 Lin T, et al. A chemical platform for improved induction of human iPSCs. Nat Methods,2009, 6(11), 805-808.
 Valamehr B, et al. A novel platform to enable the high-throughput derivation and characterization of feeder-free human iPSCs. Sci Reports,2012, 2, 213.