Vinblastine sulfate

For research use only.

Catalog No.S4505 Synonyms: NSC49842

15 publications

Vinblastine sulfate Chemical Structure

Molecular Weight(MW): 909.05

Vinblastine sulfate inhibits microtubule formation and suppresses nAChR activity with IC50 of 8.9 μM in a cell-free assay, used to treat certain kinds of cancer.

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Biological Activity

Description Vinblastine sulfate inhibits microtubule formation and suppresses nAChR activity with IC50 of 8.9 μM in a cell-free assay, used to treat certain kinds of cancer.
Targets
nAChR [1]
(Adrenal Chromaffin Cells)
8.9 μM
In vitro

The average terminal half-lives of Vinblastine is 14.3 h. When incubated in freshly isolated rat hepatocytes, VLB penetrates rapidly and intensely into the cells, probably through a passive diffusion mechanism followed by tight cellular binding[3]. Vinblastine inhibits the angiogenic response induced by adrenomedullin and is also positive for mitotic slippage, causing micronuclei in mononucleate cells with cytokinesis block[4]. vinblastine gives significant increase in micronucleated mononucleated cells at concentrations that produced approximately 50% cell death and cytostasis or less as calculated using RPD, RICC and RCC[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
COLO 320 human colorectal carcinoma cell line Mn72SpVv[3Srb36gZZN{[Xl? M1;EWGlvKH[rdILvJINwdmOnboTyZZRqd25icnXxeYlz\WRidH:gb4ltdCB3MDWgc4YhS0:OTzCzNlAhcHWvYX6gZ49td3KnY4ThcEBk[XKlaX7vcYEh[2WubDDsbY5mNCCHQ{WwQVAvODhizszN MWWxNlM3OTN7Nx?=
LNCaP human prostate cancer cell line NHG3eY1HfW6ldHnvckBie3OjeR?= Mnv2TY4hfmm2cn:gZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDrbYxtKDVyJTDv[kBNVkOjUDDoeY1idiCycn;zeIF1\SClYX7j[ZIh[2WubDDsbY5mNCCHQ{WwQVAvPSEQvF2= NHLMVI8yOjN4MUO5Oy=>
K562 cell Ml3LS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4nzNmlvKH[rdILvJIlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKGOqcn;ubYMhdXmnbH;n[Y5wfXNibHX1b4VucWFiS{W2NkBk\WyuIHfyc5d1cCxiSVO1NF0xNjByMTFOwG0> NUnEZ4dCOTJ6NUK3Olg>
T47D cells M{HVVWZ2dmO2aX;uJIF{e2G7 NFLQWmdKdiC4aYTyc{Bkd26lZX70doF1cW:wIILldZVqemWmIITvJItqdGxiNUClJI9nKFR2N1SgbJVu[W5iYoLlZZN1KGS3Y4ThcEBk[XKlaX7vcYEh[2WubDDsbY5mNCCHQ{WwQVAvODhizszN NUjuPFlvOTJ|NkGzPVc>
K562 cell MmjPVJJwdGmoZYLheIlwdiCjc4PhfS=> MYC0PEBp MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEt3NkKgZ4VtdHNiYX\0[ZIhPDhiaILzMEBKSzVyPUCuNFAyKM7:TR?= NH;xWXUyQTJ{MECxPC=>
ACHN cells M2H4[WN6fG:2b4jpZ4l1gSCjc4PhfS=> NVW2T|g2PDhiaB?= M4HjU2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGFEUE5iY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2yNk44KM7:TR?= M{TTWFE6PDZ5OEe3
A375 cells MXHDfZRwfG:6aXPpeJkh[XO|YYm= NHPoVHM1QCCq NX;yPXlyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTN5NTDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVcvOiEQvF2= NFPkfZMyQTR4N{i3Oy=>
C32 cells NWjHeYI3S3m2b4TvfIlkcXS7IHHzd4F6 NWrLZXlZPDhiaB?= NFLMSXhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBEOzJiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2zJO69VQ>? MY[xPVQ3Pzh5Nx?=
LNCAP cells NHfpcJlEgXSxdH;4bYNqfHliYYPzZZk> M1fCTlQ5KGh? NE\4d5pEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNVkODUDDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVI6NjNizszN M1zWWFE6PDZ5OEe3
Huh-7D12 cells NUL4[WZxS3m2b4TvfIlkcXS7IHHzd4F6 NYjqSXVnPDhiaB?= NHK2U49EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfWhvN1SxNkBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDJR|UxRTR3Lk[g{txO NUWwPWhJOTl2Nke4O|c>
COR-L23 cells MV3DfZRwfG:6aXPpeJkh[XO|YYm= NYHGWGV5PDhiaB?= NVvLSVNPS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hS0:ULVyyN{Bk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDJR|UxRTR3LkWg{txO NWXqblZxOTl2Nke4O|c>
142BR cells MV\DfZRwfG:6aXPpeJkh[XO|YYm= Ml\ZOFghcA>? MX3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjCxOFJDWiClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUO3MlYh|ryP MnrZNVk1Pjd6N{e=
HT-29 cells M3LoNmN6fG:2b4jpZ4l1gSCjc4PhfS=> NXrNcot4PDhiaB?= M132NWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFNiYYPzZZktKEmFNUC9NE42PSEQvF2= NIribXMzOTl{MEe2Ni=>
A549 cells NG\tW2JRem:uaX\ldoF1cW:wIHHzd4F6 NXPwb5htPDhiaB?= MlGwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGOgZZN{[XluIFnDOVA:Oi5|NjFOwG0> NHXneGwzOjV2Nk[3OC=>
DU145 cells MnHDVJJwdGmoZYLheIlwdiCjc4PhfS=> MoTSOFghcA>? MmniRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCGVUG0OUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTRwMkWg{txO M2HRVlIzPTR4Nke0
SK-MEL-5 cells MXXQdo9tcW[ncnH0bY9vKGG|c3H5 MmDLOFghcA>? MYjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFONLV3FUE02KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHMh[XO|YYmsJGlEPTB;MT63OEDPxE1? NETCTngzOjV2Nk[3OC=>
HepG2 cells Mn2xVJJwdGmoZYLheIlwdiCjc4PhfS=> Mn31OFghcA>? NIPSbHJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHMh[XO|YYmsJGlEPTB;MD6xOkDPxE1? M37SfFIzPTR4Nke0
HT-29 cells NEToZndRem:uaX\ldoF1cW:wIHHzd4F6 MVW0PEBp NGjTUWtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHMh[XO|YYmsJGlEPTB;MUGuNVgh|ryP NFftTYgzOjV2Nk[3OC=>
MCF7 cells M1iwOHBzd2yrZnXyZZRqd25iYYPzZZk> NGnFRWg1QCCq MXzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0zPC5yODFOwG0> M1m0cFIzPTR4Nke0
MDA-MB-231 cells MnXFVJJwdGmoZYLheIlwdiCjc4PhfS=> MV60PEBp MorXRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFNiYYPzZZktKEmFNUC9N|EvPTJizszN NUL4OWJyOjJ3NE[2O|Q>
rat REF52 cells NYrCRoRITnWwY4Tpc44h[XO|YYm= MXmwMlEh|ryP NY\zU2loOzBibXnudy=> NF3WXVlKdmS3Y4Tpc44hd2ZibXnjdo91fWK3bHWg[IVxd2y7bXXybZpifGmxbjDpckBz[XRiUlXGOVIh[2WubIOgZZQhOC5zIIXNJIFnfGW{IEOwJI1qdnNiYomg[ox2d3Knc3PlcoNmKGG|c3H5 NIO0bYszOzl2N{iyOi=>
HepG2 cells MmTNR5l1d3SxeHnjbZR6KGG|c3H5 MoTTO|IhcA>? NYPCTHoxS3m2b4TvfIlkcXS7IHHnZYlve3RiYXTybYFugWOrbj3y[ZNqe3SjboSgbJVu[W5iSHXwS|Ih[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlA2PiEQvF2= NHXOe|EzOzdyOECxNC=>
HepG2 cells NWL6UFJFS3m2b4TvfIlkcXS7IHHzd4F6 M2jnSVczKGh? Mn3ER5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjBzOTFOwG0> MYGyN|cxQDBzMB?=
K562 cells NV7XeIViS3m2b4TvfIlkcXS7IHHzd4F6 MmDRO|IhcA>? NHfGbVVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC5yMU[g{txO MX6yN|cxQDBzMB?=
MDA-MB-231 cells NGPtNoREgXSxdH;4bYNqfHliYYPzZZk> MWC3NkBp NGW0NpZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE4xODh|IN88US=> NUPDeFdZOjN5MEiwNVA>
MCF7 cells MnnaR5l1d3SxeHnjbZR6KGG|c3H5 NV3oN2N{PzJiaB?= NYfQcHppS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwMEC3JO69VQ>? MmLkNlM4ODhyMUC=
SCL6 cells NXXDbmZmS3m2b4TvfIlkcXS7IHHzd4F6 NX2wWlJvS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0OONjDj[YxteyCkeTDNWHQh[XO|YYmsJGVFPTB;Nj6xJO6ddQ>? NInrZ|MyOjh6MEOxOC=>
SCL9 NHHBeVZEgXSxdH;4bYNqfHliYYPzZZk> NWLzNmR[S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0OOOTDj[YxteyCkeTDNWHQh[XO|YYmsJGVFRTVwMzFOwG0> Ml\zNVI5QDB|MUS=
KATO III cells Ml7yR5l1d3SxeHnjbZR6KGG|c3H5 MoW0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2FVVyCLSVmgZ4VtdHNiYomgUXRVKGG|c3H5MEBGTDVyPU[uNUDPxE1? M4C2O|EzQDhyM{G0
NUGC4 cells MlS1R5l1d3SxeHnjbZR6KGG|c3H5 NH\BSI9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPXUeFNDDj[YxteyCkeTDNWHQh[XO|YYmsJGVFPTB;NT6zJO69VQ>? NIraOGgyOjh6MEOxOC=>
UACC903 cells MmXyR5l1d3SxeHnjbZR6KGG|c3H5 NVqzNok4PDhiaB?= M1\uR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHVCS0N7MEOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0yNjZ3IN88US=> NITKO|MzOTl{MEe2Ni=>
K562 cells M{PJN2Z2dmO2aX;uJIF{e2G7 M4PKTGlvcGmkaYTpc44hd2ZidIXieYxqdiCyb3z5cYVzcXqjdHnvckBqdiCqdX3hckBMPTZ{IHPlcIx{NCCLQ{WwQVAvOTNizszN NGPhT|YzODV2Nkm4NC=>
BxPC3 cells MWjQdo9tcW[ncnH0bY9vKGG|c3H5 NXHCdGVbPDhiaB?= M3;WXWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQojQR|Mh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD1zLkGzJO69VQ>? M1HtR|IzPTR4Nke0

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
GRP78 ; 

PubMed: 19674193     


Taxol and vinblastine induce GRP78 expression in breast cancer cells. (A) ZR75-30 cells were treated with taxol and vinblastine for 24 hrs. Cell lysates were harvested and subjected to Western blot analysis with anti-GRP78 antibody. -actin was probed as a loading control. (B) Western blot analysis of GRP78 expression in response to taxol and vinblastine treatments in T47D cells. (C) Western blot analysis of GRP78 expression in response to taxol and vinblastine treatments in MCF-7 cells. (D) MCF-7 cells were treated with 0.5 μM taxol and 0.1 μM vinblastine for indicated hours. Cell lysates were harvested and subjected to Western blot analysis with anti-GRP78 antibody.

p-eIF2; 

PubMed: 19674193     


MCF-7 cells were treated with 0.5 μM taxol and 0.1 μM vinblastine for indicated periods. Total proteins were harvested and subjected to Western blot analysis of the phosphorylated eIF2α.

p-JNK / c-Caspase-7 / c-PARP ; 

PubMed: 19674193     


MCF-7 cells were treated with indicated doses of taxol and vinblastine for 24 hrs. Total proteins were harvested and subjected to Western blot analysis of the phosphorylated JNK, cleaved caspase-7 and PARP.

ERK / p-ERK / Mcl-1 / Bad / Bid / Noxa ; 

PubMed: 20371726     


Expression of Bcl-2 family members and activation of MAPK proteins in the panel of cell lines. The indicated cell lines were either undamaged or incubated with 2.2 μM vinblastine for 4 h. Lysates were analyzed by immunoblotting for expression of the indicated proteins.

19674193 20371726
Immunofluorescence
α-tubulin / Acetyl tubulin ; 

PubMed: 30120268     


Differentiated SH-SY5Y cells were incubated in the absence and presence of indibulin (25 nM), vinblastine (10 nM) and colchicine (10 nM) for 24 h. Cells were fixed and processed for immunostaining with antibodies against α-tubulin (green) and acetylated tubulin (red). Cells were observed at two magnifications under a microscope. Scale bar = 100 and 10 μm in low (Left panel) and high (Right panel) magnification, respectively.

30120268
Growth inhibition assay
Cell viability; 

PubMed: 27114800     


Panel (A) shows cell viability (%) of the cells after 24 and 48 hrs incubation with vinblastine (0-100 nM) or docetaxel (0-100 nM). 

27114800
In vivo Vinblastine is a widely used anticancer drug with undesired side effects [6]. A combination of VBL and RAP at very low doses against human HCC gets a satisfactory antiangiogenic effect in vivo[4]. The clinically relevant dose of vinblastine inhibits palmitoylation of tubulin in vivo in CEM cells (effect on depalmitoylation of tubulin)[5].

Protocol

Cell Research:

[2]

- Collapse
  • Cell lines: Chinese hamster ovary (CHO) cells
  • Concentrations: 1% (v/v) (dissolved in DMSO)
  • Incubation Time: 3h
  • Method:

    Six-well treatment plates are set up that contained 5 × 104 cells/mL in each well, suspended in 3 mL culture medium, and these are treated with vinblastine for 3 h followed by 21 h growth.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (110.0 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 909.05
Formula

C46H58N4O9.H2SO4

CAS No. 143-67-9
Storage powder
in solvent
Synonyms NSC49842
Smiles CCC1(O)CC2CN(CCC3=C([NH]C4=CC=CC=C34)C(C2)(C(=O)OC)C5=C(OC)C=C6N(C)C7C(O)(C(OC(C)=O)C8(CC)C=CCN9CCC7(C89)C6=C5)C(=O)OC)C1.O[S](O)(=O)=O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02840409 Recruiting Drug: Vinblastine|Drug: Bevacizumab Low Grade Glioma The Hospital for Sick Children|Hoffmann-La Roche August 2016 Phase 2
NCT02670707 Recruiting Drug: Cytarabine|Drug: Vinblastine/prednisone Langerhans Cell Histiocytosis Baylor College of Medicine March 7 2016 Phase 3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID