Vinblastine sulfate

For research use only.

Catalog No.S4505 Synonyms: NSC49842

19 publications

Vinblastine sulfate Chemical Structure

CAS No. 143-67-9

Vinblastine sulfate (NSC49842) inhibits microtubule formation and suppresses nAChR activity with IC50 of 8.9 μM in a cell-free assay, used to treat certain kinds of cancer. Vinblastine sulfate induces autophagy and apoptosis.

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Biological Activity

Description Vinblastine sulfate (NSC49842) inhibits microtubule formation and suppresses nAChR activity with IC50 of 8.9 μM in a cell-free assay, used to treat certain kinds of cancer. Vinblastine sulfate induces autophagy and apoptosis.
Targets
nAChR [1]
(Adrenal Chromaffin Cells)
8.9 μM
In vitro

The average terminal half-lives of Vinblastine is 14.3 h. When incubated in freshly isolated rat hepatocytes, VLB penetrates rapidly and intensely into the cells, probably through a passive diffusion mechanism followed by tight cellular binding[3]. Vinblastine inhibits the angiogenic response induced by adrenomedullin and is also positive for mitotic slippage, causing micronuclei in mononucleate cells with cytokinesis block[4]. vinblastine gives significant increase in micronucleated mononucleated cells at concentrations that produced approximately 50% cell death and cytostasis or less as calculated using RPD, RICC and RCC[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
COLO 320 human colorectal carcinoma cell line NXTHO3FHTnWwY4Tpc44h[XO|YYm= NWnzVYRKUW5idnn0do8h[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BscWyuIEWwKUBw\iCFT1zPJFMzOCCqdX3hckBkd2yxcnXjeIFtKGOjcnPpco9u[SClZXzsJIxqdmVuIFXDOVA:OC5yODFOwG0> M1TJXFEzOzZzM{m3
LNCaP human prostate cancer cell line MYLGeY5kfGmxbjDhd5NigQ>? MUfJckB3cXS{bzDjc45k\W62cnH0bY9vKHKncYXpdoVlKHSxIHvpcIwhPTBnIH;mJGxPS2GSIHj1cYFvKHC{b4P0ZZRmKGOjbnPldkBk\WyuIHzpcoUtKEWFNUC9NE42KM7:TR?= NI\NNJoyOjN4MUO5Oy=>
K562 cell NH7IVmZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXq2WIxSUW5idnn0do8hcW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgbJVu[W5iY3jyc45q[yCveXXsc4dmdm:3czDs[ZVs\W2rYTDLOVYzKGOnbHyg[5Jwf3SqLDDJR|UxRTBwMECxJO69VQ>? NXvpVmhsOTJ6NUK3Olg>
T47D cells MlX1SpVv[3Srb36gZZN{[Xl? M{nHeWlvKH[rdILvJINwdmOnboTyZZRqd25icnXxeYlz\WRidH:gb4ltdCB3MDWgc4YhXDR5RDDoeY1idiCkcnXhd5Qh\HWldHHsJINiemOrbn;tZUBk\WyuIHzpcoUtKEWFNUC9NE4xQCEQvF2= MWOxNlM3OTN7Nx?=
K562 cell NIezfFFRem:uaX\ldoF1cW:wIHHzd4F6 MVG0PEBp NX\1b3pySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDLOVYzKGOnbHzzJIFnfGW{IES4JIhzeyxiSVO1NF0xNjByMTFOwG0> MXuxPVIzODBzOB?=
ACHN cells NFfZe5dEgXSxdH;4bYNqfHliYYPzZZk> MX[0PEBp MXHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBR2hPKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;MkKuO{DPxE1? M4W0TVE6PDZ5OEe3
A375 cells NHXNcYNEgXSxdH;4bYNqfHliYYPzZZk> M{DtT|Q5KGh? M1j5cmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE{PzViY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME23MlIh|ryP M2HCblE6PDZ5OEe3
C32 cells M1n5[mN6fG:2b4jpZ4l1gSCjc4PhfS=> NHHHZ4I1QCCq NXGxeIF2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSzN{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEmFNUC9N{DPxE1? MlPvNVk1Pjd6N{e=
LNCAP cells NXrDb2NyS3m2b4TvfIlkcXS7IHHzd4F6 MnLrOFghcA>? MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMUmNCWCClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUK5MlMh|ryP M4rGNlE6PDZ5OEe3
Huh-7D12 cells NXjXbnd[S3m2b4TvfIlkcXS7IHHzd4F6 MYe0PEBp Ml;6R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpNTeGMUKgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF01PS54IN88US=> NYX4VJFxOTl2Nke4O|c>
COR-L23 cells Mn3ER5l1d3SxeHnjbZR6KGG|c3H5 MV20PEBp MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDU3IuVDJ|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEmFNUC9OFUvPSEQvF2= MWexPVQ3Pzh5Nx?=
142BR cells NUi2[|l{S3m2b4TvfIlkcXS7IHHzd4F6 MY[0PEBp MXHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjCxOFJDWiClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUO3MlYh|ryP MnS2NVk1Pjd6N{e=
HT-29 cells NHfzW5hEgXSxdH;4bYNqfHliYYPzZZk> NEDVWG41QCCq M2rl[WN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFNiYYPzZZktKEmFNUC9NE42PSEQvF2= MV6yNVkzODd4Mh?=
A549 cells M1nOeXBzd2yrZnXyZZRqd25iYYPzZZk> M2PV[VQ5KGh? M4nQd2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTJwM{[g{txO MmrWNlI2PDZ4N{S=
DU145 cells NF\UPGtRem:uaX\ldoF1cW:wIHHzd4F6 MWW0PEBp M3fUPGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRGWxOFUh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD12LkK1JO69VQ>? MWeyNlU1PjZ5NB?=
SK-MEL-5 cells MnLsVJJwdGmoZYLheIlwdiCjc4PhfS=> MnnNOFghcA>? NV7GdpRESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTT{1OTUxvNTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwQVEvPzRizszN NEjoTWszOjV2Nk[3OC=>
HepG2 cells M3XqWnBzd2yrZnXyZZRqd25iYYPzZZk> M{DLU|Q5KGh? MnzJRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZYDHNkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTBwMU[g{txO M3fObFIzPTR4Nke0
HT-29 cells NF7VOHpRem:uaX\ldoF1cW:wIHHzd4F6 MmHGOFghcA>? NV;q[3pOSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRUKGG|c3H5MEBKSzVyPUGxMlE5KM7:TR?= MoPINlI2PDZ4N{S=
MCF7 cells NWrleYhnWHKxbHnm[ZJifGmxbjDhd5NigQ>? MYS0PEBp Mn[0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGOgZZN{[XluIFnDOVA:OjRwMEig{txO MUKyNlU1PjZ5NB?=
MDA-MB-231 cells M3e2OHBzd2yrZnXyZZRqd25iYYPzZZk> M4TiSVQ5KGh? MmrnRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFNiYYPzZZktKEmFNUC9N|EvPTJizszN NXzLSHdyOjJ3NE[2O|Q>
rat REF52 cells MniySpVv[3Srb36gZZN{[Xl? MlHWNE4yKM7:TR?= NULWfWxsOzBibXnudy=> MmK5TY5lfWO2aX;uJI9nKG2rY4LveJVjfWynIHTldI9tgW2ncnn6ZZRqd25iaX6gdoF1KFKHRkWyJINmdGy|IHH0JFAvOSC3TTDh[pRmeiB|MDDtbY5{KGK7IH\seY9z\XOlZX7j[UBie3OjeR?= NV;IXG56OjN7NEe4NlY>
HepG2 cells NVP3RoVNS3m2b4TvfIlkcXS7IHHzd4F6 NH3pbJk4OiCq M2\Ub2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGGmcnnhcZlkcW5vcnXzbZN1[W62IHj1cYFvKEincFeyJINmdGy|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6wOVYh|ryP MlzCNlM4ODhyMUC=
HepG2 cells NFvOWoJEgXSxdH;4bYNqfHliYYPzZZk> MoLoO|IhcA>? M{LtUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC5yMUmg{txO NXv3XlJQOjN5MEiwNVA>
K562 cells MkTvR5l1d3SxeHnjbZR6KGG|c3H5 MlXnO|IhcA>? Mn7BR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvODF4IN88US=> NXnIS3VUOjN5MEiwNVA>
MDA-MB-231 cells NWrwSG1uS3m2b4TvfIlkcXS7IHHzd4F6 M37FZVczKGh? Mk\NR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuNFA5OyEQvF2= NWfkNpE6OjN5MEiwNVA>
MCF7 cells NEHnU3FEgXSxdH;4bYNqfHliYYPzZZk> NVrVPFY{PzJiaB?= M1LHOWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjByNzFOwG0> M2DzeFI{PzB6MEGw
SCL6 cells M2DNUmN6fG:2b4jpZ4l1gSCjc4PhfS=> NWTrSFlJS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0OONjDj[YxteyCkeTDNWHQh[XO|YYmsJGVFPTB;Nj6xJO6ddQ>? Mk\0NVI5QDB|MUS=
SCL9 NVyzPVczS3m2b4TvfIlkcXS7IHHzd4F6 MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTR2w6KGOnbHzzJIJ6KE2WVDDhd5NigSxiRVS9OU4{KM7:TR?= Mlv3NVI5QDB|MUS=
KATO III cells MVjDfZRwfG:6aXPpeJkh[XO|YYm= MljuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2FVVyCLSVmgZ4VtdHNiYomgUXRVKGG|c3H5MEBGTDVyPU[uNUDPxE1? MojuNVI5QDB|MUS=
NUGC4 cells NGPHU45EgXSxdH;4bYNqfHliYYPzZZk> NUTVO4J7S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVlWJQ{SgZ4VtdHNiYomgUXRVKGG|c3H5MEBGTDVyPUWuN{DPxE1? MV:xNlg5ODNzNB?=
UACC903 cells NUP4PW5XS3m2b4TvfIlkcXS7IHHzd4F6 NICwNHk1QCCq M37CdGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHVCS0N7MEOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0yNjZ3IN88US=> NXHU[nR[OjF7MkC3OlI>
K562 cells NXLmZYNbTnWwY4Tpc44h[XO|YYm= NVvQUo12UW6qaXLpeIlwdiCxZjD0eYJ2dGmwIIDvcJlu\XKrenH0bY9vKGmwIHj1cYFvKEt3NkKgZ4VtdHNuIFnDOVA:OC5zMzFOwG0> M4XWS|IxPTR4OUiw
BxPC3 cells M4XTSHBzd2yrZnXyZZRqd25iYYPzZZk> MX60PEBp MlP6RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEeGDDN{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTFwMUOg{txO MkLWNlI2PDZ4N{S=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
GRP78 ; 

PubMed: 19674193     


Taxol and vinblastine induce GRP78 expression in breast cancer cells. (A) ZR75-30 cells were treated with taxol and vinblastine for 24 hrs. Cell lysates were harvested and subjected to Western blot analysis with anti-GRP78 antibody. -actin was probed as a loading control. (B) Western blot analysis of GRP78 expression in response to taxol and vinblastine treatments in T47D cells. (C) Western blot analysis of GRP78 expression in response to taxol and vinblastine treatments in MCF-7 cells. (D) MCF-7 cells were treated with 0.5 μM taxol and 0.1 μM vinblastine for indicated hours. Cell lysates were harvested and subjected to Western blot analysis with anti-GRP78 antibody.

p-eIF2; 

PubMed: 19674193     


MCF-7 cells were treated with 0.5 μM taxol and 0.1 μM vinblastine for indicated periods. Total proteins were harvested and subjected to Western blot analysis of the phosphorylated eIF2α.

p-JNK / c-Caspase-7 / c-PARP ; 

PubMed: 19674193     


MCF-7 cells were treated with indicated doses of taxol and vinblastine for 24 hrs. Total proteins were harvested and subjected to Western blot analysis of the phosphorylated JNK, cleaved caspase-7 and PARP.

ERK / p-ERK / Mcl-1 / Bad / Bid / Noxa ; 

PubMed: 20371726     


Expression of Bcl-2 family members and activation of MAPK proteins in the panel of cell lines. The indicated cell lines were either undamaged or incubated with 2.2 μM vinblastine for 4 h. Lysates were analyzed by immunoblotting for expression of the indicated proteins.

19674193 20371726
Immunofluorescence
α-tubulin / Acetyl tubulin ; 

PubMed: 30120268     


Differentiated SH-SY5Y cells were incubated in the absence and presence of indibulin (25 nM), vinblastine (10 nM) and colchicine (10 nM) for 24 h. Cells were fixed and processed for immunostaining with antibodies against α-tubulin (green) and acetylated tubulin (red). Cells were observed at two magnifications under a microscope. Scale bar = 100 and 10 μm in low (Left panel) and high (Right panel) magnification, respectively.

30120268
Growth inhibition assay
Cell viability; 

PubMed: 27114800     


Panel (A) shows cell viability (%) of the cells after 24 and 48 hrs incubation with vinblastine (0-100 nM) or docetaxel (0-100 nM). 

27114800
In vivo Vinblastine is a widely used anticancer drug with undesired side effects [6]. A combination of VBL and RAP at very low doses against human HCC gets a satisfactory antiangiogenic effect in vivo[4]. The clinically relevant dose of vinblastine inhibits palmitoylation of tubulin in vivo in CEM cells (effect on depalmitoylation of tubulin)[5].

Protocol

Cell Research:

[2]

- Collapse
  • Cell lines: Chinese hamster ovary (CHO) cells
  • Concentrations: 1% (v/v) (dissolved in DMSO)
  • Incubation Time: 3h
  • Method:

    Six-well treatment plates are set up that contained 5 × 104 cells/mL in each well, suspended in 3 mL culture medium, and these are treated with vinblastine for 3 h followed by 21 h growth.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (110.0 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 909.05
Formula

C46H58N4O9.H2SO4

CAS No. 143-67-9
Storage powder
in solvent
Synonyms NSC49842
Smiles CCC1(CC2CC(C3=C(CCN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC)O.OS(=O)(=O)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02840409 Recruiting Drug: Vinblastine|Drug: Bevacizumab Low Grade Glioma The Hospital for Sick Children|Hoffmann-La Roche August 2016 Phase 2
NCT02670707 Recruiting Drug: Cytarabine|Drug: Vinblastine/prednisone Langerhans Cell Histiocytosis Baylor College of Medicine March 7 2016 Phase 3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID