Home Proteases MMP inhibitor - Interleukins inhibitor - ROS inhibitor Triolein For research use only.

Triolein

Triolein is an inhibitor of metalloproteinase-1 (MMP-1) and reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes.

Triolein Chemical Structure

Triolein Chemical Structure

CAS: 122-32-7

Purity & Quality Control

Batch: Purity: 100%
100

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Signaling Pathway

MMP Signaling Pathways

MMP Signaling Pathway

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Biological Activity

Description Triolein is an inhibitor of metalloproteinase-1 (MMP-1) and reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes.
Targets
MMP-1 [1] IL-6 [1] ROS [1]
In vitro
In vitro

Triolein is an inhibitor of metalloproteinase-1 (MMP-1) and reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes, which can be proposed as an active agent against skin photodamage.[1]
Cell Research Cell lines The immortalized human bulge stem cell line Tel‐E6E7, Human dermal fibroblasts
Concentrations 20 µM
Incubation Time 24 h
Method

Tel‐E6E7 cells were seeded on day one at a density of 2000 cells per cm2 on 3T3‐swiss cells feeder layer in cFAD medium. On day five the medium was change to DMEM and F12 (1:1) mixture containing 2 mM glutamine, hydrocortisone (0.4 µg/ml), 2 mM penicillin/streptomycin, Epithelial Growth factor (5 ng/ml) and 10 % fetal bovine serum. On day six the cells were irradiated on PBS using a UVM‐57 UVP handheld UV lamp (302 nm Midrange) at sub‐lethal doses of 3.4 or 6.8 mJ/cm2. Immediately, PBS was replaced with medium containing triolein 20 µM. 24 hours later mRNA was extracted from cells using Trizol Reagent. Conditioned culture medium was collected and used to replace the medium of dermal fibroblasts cells plated the day before (75000 cells/cm2) in DMEM supplemented with 10% fetal bovine serum and 2 mM glutamine. After 48 hours dermal fibroblasts mRNA was extracted.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01128400 Completed
Obesity
Washington University School of Medicine|GlaxoSmithKline
 May 2010 --

Chemical Information & Solubility

Molecular Weight 885.43 Formula

C57H104O6
CAS No. 122-32-7 SDF --
Density 0.908 g/mL
Smiles CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COC(=O)CCCCCCC\C=C/CCCCCCCC)OC(=O)CCCCCCC\C=C/CCCCCCCC
Storage (From the date of receipt) 2 years -20°C liquid

In vitro
Batch:

DMSO : 100 mg/mL ( (112.93 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 6 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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