Saquinavir (Ro 31-8959)

Catalog No.S9625

For research use only.

Saquinavir (Ro 31-8959) is a potent and selective inhibitor of HIV proteinase with a mean IC50 of 2.7 nM in JM cells.

Saquinavir (Ro 31-8959) Chemical Structure

CAS No. 127779-20-8

Purity & Quality Control

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Biological Activity

Description Saquinavir (Ro 31-8959) is a potent and selective inhibitor of HIV proteinase with a mean IC50 of 2.7 nM in JM cells.
HIV proteinase [1]
(in JM cells)
2.7 nM
In vitro

Saquinavir (Ro 31-8959) is an effective selective HIV protease inhibitor with an average IC50 of 2.7 nM in JM cells, which inhibits the spread of HIV infection and the production of cytopathic effects.<sup><a class="sref" href="#s_ref">[1]</a></sup>

Protocol (from reference)

Cell Research:

<sup><a class="sref" href="#s_ref">[1]</a></sup>

  • Cell lines: JM cells
  • Concentrations: 0.5-100 nM
  • Incubation Time: 3 days
  • Method:

    JM cells (2 × 10<sup>5</sup> cells/ml) were exposed to HIV-1 (GB8) at a multiplicity of infection (MOI) of 0.01 syncytium-forming units/cell. Aliquots (0.2 ml) of cells were placed in microtitre wells with appropriate dilutions of Ro 31-8959 added to 5 replicate cultures. After incubation for 3 days at 37°C in a humidified atmosphere of 5% CO2 in air, syncytia were counted.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 670.84


CAS No. 127779-20-8
Storage 3 years -20°C powder
2 years -80°C in solvent

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00623597 Completed Drug: ritonavir|Drug: saquinavir [Invirase] HIV Infections Hoffmann-La Roche June 2008 Phase 2
NCT00622206 Completed Drug: ritonavir and saquinavir HIV Infections The HIV Netherlands Australia Thailand Research Collaboration January 2008 Phase 1|Phase 2
NCT00435929 Completed Drug: Ritonavir|Drug: saquinavir [Invirase] HIV Infections Hoffmann-La Roche September 2006 Phase 1
NCT00389402 Completed Drug: saquinavir/ritonavir|Drug: atazanavir/ritonavir HIV Infections International Antiviral Therapy Evaluation Center|Hoffmann-La Roche|Gilead Sciences July 2006 Phase 4
NCT00379405 Completed Drug: Saquinavir/Ritonavir : 2 capsules (500 mg) / 12 hours HIV Infections Germans Trias i Pujol Hospital|Fundación FLS de Lucha Contra el Sida las Enfermedades Infecciosas y la Promoción de la Salud y la Ciencia June 2006 Phase 4

(data from, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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