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Azvudine HIV Protease inhibitor

Name: Azvudine
Brand: Selleck Chemicals
SKU: E1243
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.E1243

Azvudine (RO-0622) is a novel nucleoside reverse transcriptase inhibitor with antiviral activity against human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HCV), potently inhibits HIV-1 (EC50 range 0.03 to 6.92 nM) and HIV-2 (EC50s ranging from 0.018 to 0.025 nM).

Chemical Structure

Molecular Weight: 286.22

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Quality Control

Batch: E124301 DMSO]57 mg/mL]false]Water]57 mg/mL]false]Ethanol]57 mg/mL]false Purity: 99.86%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS/MSDS
Datasheet

99.86

Related Targets	Integrase Bacterial Antibiotics Anti-infection Fungal Antiviral COVID-19 Parasite Reverse Transcriptase HIV
Other HIV Protease Inhibitors	Pepstatin A Limonin Temsavir (BMS-626529) Dextran sulfate sodium (DSS) Bevirimat Rosamultin Mericitabine NBD-556 GS-6207 (Lenacapavir)

Solubility

In vitro
Batch:

DMSO : 57 mg/mL (199.14 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 57 mg/mL

Ethanol : 57 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.850mg/ml (9.96mM)	Taking the 1 mL working solution as an example, add 50 μL of 57 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.470mg/ml (1.64mM)	Taking the 1 mL working solution as an example, add 50 μL of 9.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	286.22	Formula	C₉H₁₁FN₆O₄	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	1011529-10-4	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Read more about storage stability stock solution CAS number

Mechanism of Action

Targets/IC₅₀/Ki	HIV-1 (in antiviral activity assays) 0.03~ 6.92 nM(EC50) HIV-2 (in antiviral activity assays) 0.018~ 0.025 nM(EC50) HBV HCV
References	[1] https://pubmed.ncbi.nlm.nih.gov/25144636/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2025-12-29)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07307586	RECRUITING	Chronic Hepatitis B; Azvudine	Zhigang Ren	2025-09-01	PHASE3
NCT07001436	RECRUITING	Renal Insufficiency	Henan Genuine Biotech Co., Ltd.	2025-05-20	PHASE1
NCT05689034	UNKNOWN	COVID-19 Respiratory Infection	Peking Union Medical College Hospital	2023-04-19	PHASE2; PHASE3
NCT07002281	COMPLETED	Healthy	Henan Genuine Biotech Co., Ltd.	2024-07-22	PHASE1
NCT05633433	UNKNOWN	SARS-CoV-2 Infection	Shanghai Henlius Biotech	2022-12-29	PHASE2; PHASE3
NCT05697055	UNKNOWN	COVID-19	Shandong Provincial Hospital	2023-01-21	PHASE4

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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