MLN120B

Catalog No.S7736 Synonyms: ML120B

For research use only.

MLN120B (ML120B) is a potent, selective, reversible, and ATP-competitive inhibitor of IkappaB kinase beta (IKKβ) with an IC50 of 45 nM for recombinant IKKβ. MLN120B does not inhibit other IKK isoforms at IC50 below 50 μM.

MLN120B Chemical Structure

CAS No. 783348-36-7

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Biological Activity

Description MLN120B (ML120B) is a potent, selective, reversible, and ATP-competitive inhibitor of IkappaB kinase beta (IKKβ) with an IC50 of 45 nM for recombinant IKKβ. MLN120B does not inhibit other IKK isoforms at IC50 below 50 μM.
Targets
recombinant IKKβ [1]
45 nM
In vitro

MLN120B, a anti-inflammatory compound, is a NF-kB pathway blocker of inhibiting IKK2, and shows an inhibitory effect on LPS induced NF-kB activation. The IC50 value of MLN120B is 1.4, 14.8 or 27.3 μM for NF-kB2-luc2, IL8-luc2 or TNF-AIP3-luc2 reporter transfected cells, respectively, indicating that IKK2 inhibition affects the transcriptional activity of the NF-kB2 promoter most effective.<sup><a class="sref" href="#s_ref">[1]</a></sup><sup><a class="sref" href="#s_ref">[2]</a></sup>

In vivo

Significantly decreased levels of the pro-inflammatory chemokine MIP-1β and increased levels of the anti-inflammatory cytokine IL-10 were detected in the BAL of MLN120B pre-treated mice as opposed to LPS-treated positive controls.<sup><a class="sref" href="#s_ref">[2]</a></sup>

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: TNFAIP3-luc2, NF-kB2-luc2 and IL8-luc2 transfected RAW264.7 cells
  • Concentrations: 1-100 µM
  • Incubation Time: --
  • Method:

    Transiently transfect the NF-kB reporters into RAW267.4 cells and induce inflammation in the cells with LPS. The effect of these compounds on LPS induced luciferase expression was assayed. 

Animal Research:

[2]

  • Animal Models: NF-kB2-luc2 transfected mice
  • Dosages: 300 mg/kg
  • Administration: p.o.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 366.80
Formula

C19H15ClN4O2

CAS No. 783348-36-7
Storage 3 years -20°C powder
2 years -80°C in solvent

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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