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Minocycline Hydrochloride Antibiotic

Cat.No.S4226

Minocycline HCl is the most lipid soluble and most active tetracycline antibiotic, binds to the 30S ribosomal subunit, preventing the binding of tRNA to the mRNA-ribosome complex and interfering with protein synthesis.
Minocycline Hydrochloride Antibiotics chemical Chemical Structure

Chemical Structure

Molecular Weight: 493.94

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Quality Control

Batch: Purity: 99.94%
99.94

Cell Culture, Treatment & Working Concentration

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jurkat cells Cell viability assay 10-200 µM 24 h reduces cell viability and induces DNA fragmentation and dissipation of the mitochondrial membrane potential in Jurkat cells but is harmless to human peripheral blood lymphocyte cells (hPBLCs)
hPBLCs Cell viability assay 10-200 µM 24 h reduces cell viability and induces DNA fragmentation and dissipation of the mitochondrial membrane potential in Jurkat cells but is harmless to human peripheral blood lymphocyte cells (hPBLCs)
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Solubility

In vitro
Batch:

Water : 6 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

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In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Chemical Information, Storage & Stability

Molecular Weight 493.94 Formula

C23H27N3O7.HCl

 Storage (From the date of receipt)
CAS No. 13614-98-7 Download SDF Storage of Stock Solutions

Synonyms CL 59806 SMILES CN(C)C1C2CC3CC4=C(C=CC(=C4C(=C3C(=O)C2(C(=C(C1=O)C(=O)N)O)O)O)O)N(C)C.Cl

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro

Minocycline is a second-generation tetracycline used in humans, which effectively crosses the blood−brain barrier. It inhibits the activity of caspase-1, caspase-3, inducible form of nitric oxide synthetase (iNOS) and p38 mitogen-activated protein kinase (MAPK). After experimental ischemia, minocycline inhibits caspase-1 and inducible nitric oxide synthetase (iNOS) upregulation, and decreases infarct size. Minocycline inhibits mitochondrial permeability-transition-mediated cytochrome c release. Minocycline-mediated inhibition of cytochrome c release is demonstrated in vivo, in cells, and in isolated mitochondria.

In vivo

Minocycline is a semi-synthetic tetracycline derivative which is well absorbed and distributed in body tissues and is suitable for twice daily administration. Minocycline's effects are related to the inhibition of protein synthesis. Although minocycline's broader spectrum of activity, compared to other members of the group, includes activity against Neisseria meningitidis, its use as a prophylaxis is no longer recomended because of side effects (dizziness and vertigo).

Minocycline is remarkable neuroprotective qualities in models of cerebral ischaemia, traumatic brain injury, and Huntington's and Parkinson's disease. The neuroprotective action of minocycline may include its inhibitory effect on 5-lipoxygenase, an inflammatory enzyme associated with brain aging.

References
  • [4] https://pubmed.ncbi.nlm.nih.gov/22252097/

Applications

Methods Biomarkers Images PMID
Western blot IL-6Rα / gp130 p-STAT3 / STAT3 / Mcl-1 / p-ERK / ERK
S4226-WB1
23593315
Immunofluorescence p-STAT3 STAT3
S4226-IF1
23593315
Growth inhibition assay Cell viability
S4226-viability1
27555377

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-07-30)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT07735819 NOT_YET_RECRUITING
Low Grade Glioma; Glioblastoma
Barbara Ann Karmanos Cancer Institute
 2026-09 PHASE2
NCT06699966 NOT_YET_RECRUITING
Major Depressive Disorder
Stony Brook University
 2027-06 PHASE4
NCT07305896 NOT_YET_RECRUITING
Intracerebral Hemorrhage
The First Hospital of Jilin University
 2025-12-31
NCT07526987 RECRUITING
Ischaemic Stroke
Beijing Tiantan Hospital
 2026-06-05 PHASE3
NCT07237048 RECRUITING
Stroke; Ischemia Stroke; Hemorrhagic Strokes; Mortality; Morbidity; Cerebrovascular Accident (Stroke); Intracerebral Haemorrhage (ICH)
Joyce Chen
 2026-02-02 PHASE2; PHASE3
NCT07594301 RECRUITING
Acute Ischemic Stroke; Endovascular Thrombectomy; Minocycline; Posterior Circulation; Arterial Occlusion
Xiang Luo
 2026-05-19 PHASE2; PHASE3

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