Minocycline (CL 59806) HCl

Catalog No.S4226

For research use only.

Minocycline (CL 59806) HCl is the most lipid soluble and most active tetracycline antibiotic, binds to the 30S ribosomal subunit, preventing the binding of tRNA to the mRNA-ribosome complex and interfering with protein synthesis.

Minocycline (CL 59806) HCl Chemical Structure

CAS No. 13614-98-7

Selleck's Minocycline (CL 59806) HCl has been cited by 27 publications

Purity & Quality Control

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Biological Activity

Description Minocycline (CL 59806) HCl is the most lipid soluble and most active tetracycline antibiotic, binds to the 30S ribosomal subunit, preventing the binding of tRNA to the mRNA-ribosome complex and interfering with protein synthesis.
In vitro

Minocycline is a second-generation tetracycline used in humans, which effectively crosses the blood−brain barrier. It inhibits the activity of caspase-1, caspase-3, inducible form of nitric oxide synthetase (iNOS) and p38 mitogen-activated protein kinase (MAPK). After experimental ischemia, minocycline inhibits caspase-1 and inducible nitric oxide synthetase (iNOS) upregulation, and decreases infarct size. Minocycline inhibits mitochondrial permeability-transition-mediated cytochrome c release. Minocycline-mediated inhibition of cytochrome c release is demonstrated in vivo, in cells, and in isolated mitochondria. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jurkat cells NInr[ZNE\WyuII\pZYJqdGm2eTDhd5NigQ>? NF30Sm8yOC1{MECgxtVO NIPkTGozPCCq NVXU[GQ6emWmdXPld{Bk\WyuII\pZYJqdGm2eTDhcoQhcW6mdXPld{BFVkFiZoLh[41mdnSjdHnvckBidmRiZHnzd4lx[XSrb36gc4YhfGinIH3peI9kcG:wZILpZYwhdWWvYoLhcoUheG:2ZX70bYFtKGmwIFr1dotifCClZXzsd{BjfXRiaYOgbIFzdWync4OgeI8hcHWvYX6gdIVzcXCqZYLhcEBjdG:xZDDsfY1xcG:leYTlJINmdGy|IDjoVGJNS3Nr NF7MbJIzQTZ{NUG2OS=>
hPBLCs NIjPXItE\WyuII\pZYJqdGm2eTDhd5NigQ>? MnXZNVAuOjByINM1US=> NHTB[W4zPCCq NEjZZmFz\WS3Y3XzJINmdGxidnnhZoltcXS7IHHu[EBqdmS3Y3XzJGRPSSCocnHncYVvfGG2aX;uJIFv\CCmaYPzbZBifGmxbjDv[kB1cGVibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBxd3SnboTpZYwhcW5iSoXyb4F1KGOnbHzzJIJ2fCCrczDoZZJudGW|czD0c{BpfW2jbjDw[ZJqeGincnHsJIJtd2:mIHz5cZBpd2O7dHWgZ4VtdHNiKHjQRmxEeyl? NVHOcI1rOjl4MkWxOlU>
Methods Test Index PMID
Western blot IL-6Rα / gp130 ; p-STAT3 / STAT3 / Mcl-1 / p-ERK / ERK 23593315
Immunofluorescence p-STAT3 ; STAT3 23593315
Growth inhibition assay Cell viability 27555377
In vivo Minocycline is a semi-synthetic tetracycline derivative which is well absorbed and distributed in body tissues and is suitable for twice daily administration. Minocycline's effects are related to the inhibition of protein synthesis. Although minocycline's broader spectrum of activity, compared to other members of the group, includes activity against Neisseria meningitidis, its use as a prophylaxis is no longer recomended because of side effects (dizziness and vertigo). [1] Minocycline is remarkable neuroprotective qualities in models of cerebral ischaemia, traumatic brain injury, and Huntington's and Parkinson's disease. The neuroprotective action of minocycline may include its inhibitory effect on 5-lipoxygenase, an inflammatory enzyme associated with brain aging. [3]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 493.94


CAS No. 13614-98-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN(C)C1C2CC3CC4=C(C=CC(=C4C(=C3C(=O)C2(C(=C(C1=O)C(=O)N)O)O)O)O)N(C)C.Cl

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04202263 Recruiting Drug: Minocycline Topical|Drug: Placebos Allergic Rhinitis|Asthma State University of New York - Downstate Medical Center September 26 2019 Phase 2
NCT03291158 Unknown status Drug: Minocycline Normal Healthy Volunteers Rempex Pharmaceuticals (a wholly owned subsidiary of The Medicines Company)|Universitätsklinikum Köln|Innovative Medicines Initiative April 2018 Phase 1
NCT03160040 Completed Drug: Minocycline IV Gram-Negative Bacterial Infections Rempex (a wholly owned subsidiary of Melinta Therapeutics Inc.)|Melinta Therapeutics Inc. October 11 2017 --
NCT02808052 Terminated Drug: Minocin (minocycline) for Injection Renal Insufficiency Acute|Renal Insufficiency Chronic|Healthy Subjects Rempex (a wholly owned subsidiary of Melinta Therapeutics Inc.)|Universitätsklinikum Köln|Innovative Medicines Initiative|Melinta Therapeutics Inc. May 29 2017 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

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