Minocycline HCl

Catalog No.S4226 Batch:S422605

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Technical Data

Formula

C23H27N3O7.HCl

Molecular Weight 493.94 CAS No. 13614-98-7
Solubility (25°C)* In vitro Water 14 mg/mL (28.34 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Minocycline HCl is the most lipid soluble and most active tetracycline antibiotic, binds to the 30S ribosomal subunit, preventing the binding of tRNA to the mRNA-ribosome complex and interfering with protein synthesis.
In vitro

Minocycline is a second-generation tetracycline used in humans, which effectively crosses the blood−brain barrier. It inhibits the activity of caspase-1, caspase-3, inducible form of nitric oxide synthetase (iNOS) and p38 mitogen-activated protein kinase (MAPK). After experimental ischemia, minocycline inhibits caspase-1 and inducible nitric oxide synthetase (iNOS) upregulation, and decreases infarct size. Minocycline inhibits mitochondrial permeability-transition-mediated cytochrome c release. Minocycline-mediated inhibition of cytochrome c release is demonstrated in vivo, in cells, and in isolated mitochondria. [2]

In vivo

Minocycline is a semi-synthetic tetracycline derivative which is well absorbed and distributed in body tissues and is suitable for twice daily administration. Minocycline's effects are related to the inhibition of protein synthesis. Although minocycline's broader spectrum of activity, compared to other members of the group, includes activity against Neisseria meningitidis, its use as a prophylaxis is no longer recomended because of side effects (dizziness and vertigo). [1]

Minocycline is remarkable neuroprotective qualities in models of cerebral ischaemia, traumatic brain injury, and Huntington's and Parkinson's disease. The neuroprotective action of minocycline may include its inhibitory effect on 5-lipoxygenase, an inflammatory enzyme associated with brain aging. [3]

Protocol (from reference)

Cell Assay:

[4]

  • Cell lines

    OVCAR-3, SKOV-3 and A2780 cells

  • Concentrations

    IC50 values of 62.0, 56.1 and 59.5 μM, respectively

  • Incubation Time

    72 h

  • Method

    Cells were treated with various concentrations of drug for 72 h.

Selleck's Minocycline HCl has been cited by 34 publications

Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm [ Protein Cell, 2023, 14(4):262-278] PubMed: 37084236
Comparative assessment of embryotoxicity of 2,4,6-triiodophenol to mouse blastoid and pre-implantation embryo models [ Ecotoxicol Environ Saf, 2023, 252:114608] PubMed: 36738612
Adolescent microglia stimulation produces long-lasting protection against chronic stress-induced behavioral abnormalities in adult male mice [ Brain Behav Immun, 2022, 105:44-66] PubMed: 35781008
Innate immune stimulation by monophosphoryl lipid A prevents chronic social defeat stress-induced anxiety-like behaviors in mice [ J Neuroinflammation, 2022, 19(1):12] PubMed: 34996472
Intratarget Microdosing for Deep Phenotyping of Multiple Drug Effects in the Live Brain [ Front Bioeng Biotechnol, 2022, 10:855755] PubMed: 35372313
Microglial Activation Damages Dopaminergic Neurons through MMP-2/-9-Mediated Increase of Blood-Brain Barrier Permeability in a Parkinson's Disease Mouse Model [ Int J Mol Sci, 2022, 23(5)2793] PubMed: 35269933
Granulocyte-macrophage colony-stimulating factor-triggered innate immune tolerance against chronic stress-induced behavioral abnormalities in mice [ Int Immunopharmacol, 2022, 109:108924] PubMed: 35704970
Protein Expression Landscape Defines the Formation Potential of Mouse Blastoids From EPSCs [ Front Cell Dev Biol, 2022, 10:840492] PubMed: 35211474
Protective Effect of Minocycline Hydrochloride on the Mouse Embryonic Development Against Suboptimal Environment [ Front Cell Dev Biol, 2022, 10:799042] PubMed: 35178387
Monophosphoryl lipid A tolerance against chronic stress-induced depression-like behaviors in mice [ Int J Neuropsychopharmacol, 2022, pyab097] PubMed: 35015863

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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