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Technical Data
| Formula | C20H38N6O4.1/2H2SO4 |
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| Molecular Weight | 475.59 | CAS No. | 103476-89-7 | |
| Solubility (25°C)* | In vitro | DMSO | 95 mg/mL (199.75 mM) | |
| Water | 95 mg/mL (199.75 mM) | |||
| Ethanol | 95 mg/mL (199.75 mM) | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Preparing Stock Solutions
Biological Activity
| Description | Leupeptin Hemisulfate is a reversible inhibitor of serine and cysteine proteases. It inhibits cathepsin B (Ki = 6 nM), calpain (Ki = 10 nM), trypsin (Ki = 35 nM), plasmin (Ki = 3.4 μM), and kallikrein (Ki = 19 μM), and has no effect against chymotrypsin, elastase, renin, or pepsin. | ||
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| Targets |
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| In vitro | Leupeptin, produced by various species of actinomycetes, strongly inhibit proteolysis. [1] Leupeptin hemisulfate protects tubulin from endogenous proteolytic activities during the isolation procedure and results in increased tubulin purity. [2] Leupeptin hemisulfate could restore up to 50% of hepatitis B surface antigen (HBsAg) expression in cell suspension cultures. [3] |
Protocol (from reference)
| Animal Study: |
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References
Selleck's Leupeptin Hemisulfate has been cited by 30 publications
| ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade [ Nat Commun, 2023, 14(1):4066] | PubMed: 37429863 |
| Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway [ Acta Pharm Sin B, 2022, 12(6):2869-2886] | PubMed: 35755273 |
| 18F-FDG PET as an imaging biomarker for the response to FGFR-targeted therapy of cancer cells via FGFR-initiated mTOR/HK2 axis [ Theranostics, 2022, 12(14):6395-6408] | PubMed: 36168616 |
| CLP36 promotes p53 deficient sarcoma progression through suppression of atrophin-1 interacting protein-4 (AIP-4)-dependent degradation of YAP1 [ Theranostics, 2022, 12(11):5051-5068] | PubMed: 35836803 |
| Histone deacetylase 3 contributes to the antiviral innate immunity of macrophages by interacting with FOXK1 to regulate STAT1/2 transcription [ Cell Rep, 2022, 38(4):110302] | PubMed: 35081346 |
| Histone deacetylase 3 facilitates TNFα-mediated NF-κB activation through suppressing CTSB induced RIP1 degradation and is required for host defense against bacterial infection [ Cell Biosci, 2022, 12(1):81] | PubMed: 35658939 |
| Potential drug discovery for COVID-19 treatment targeting Cathepsin L using a deep learning-based strategy [ Comput Struct Biotechnol J, 2022, 20:2442-2454] | PubMed: 35602976 |
| Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model [ Protein Cell, 2021, 10.1007/s13238-021-00858-3] | PubMed: 34291435 |
| ACLY ubiquitination by CUL3-KLHL25 induces the reprogramming of fatty acid metabolism to facilitate iTreg differentiation [ Elife, 2021, 10e62394] | PubMed: 34491895 |
| USP9X deubiquitinates connexin43 to prevent high glucose-induced epithelial-to-mesenchymal transition in NRK-52E cells [ Biochem Pharmacol, 2021, 188:114562] | PubMed: 33857489 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.