Jatrorrhizine

Catalog No.S3884 Synonyms: Neprotin, Yatrorizine

For research use only.

Jatrorrhizine (Neprotin, Yatrorizine), one of the active constituents of Coptis chinensis Franch, has multiple bioactivities, such as hypoglycemic, antimicrobial, and antioxidant activities. It is an inhibitor of AChE with IC50 of 872 nM and demonstrates >115-fold selectivity for AChE over BuChE.

Jatrorrhizine Chemical Structure

CAS No. 3621-38-3

Purity & Quality Control

Choose Selective AChR Inhibitors

Other AChR Products

Biological Activity

Description Jatrorrhizine (Neprotin, Yatrorizine), one of the active constituents of Coptis chinensis Franch, has multiple bioactivities, such as hypoglycemic, antimicrobial, and antioxidant activities. It is an inhibitor of AChE with IC50 of 872 nM and demonstrates >115-fold selectivity for AChE over BuChE.
Targets
AChE [1]
(Cell-free assay)
872 nM
In vitro

Jatrorrhizine hydrochloride suppresses C8161 cell proliferation in a dose-dependent manner, with a half-maximal inhibitory concentration of 47.4±1.6 μmol/l. however, it does not induce significant cellular apoptosis at doses up to 320 μmol/l. Mechanistic studies show that JH-induced C8161 cell cycle arrest at the G0/G1 transition, which is accompanied by overexpression of the cell cycle-suppressive genes p21 and p27 at higher doses[2].

In vivo JH reduces metastatic melanoma cell-mediated neovascularization[2]. JH ameliorates hyperlipidemia via the suppression of lipogenesis and the enhancement of lipid oxidation in the liver[3].

Protocol (from reference)

Cell Research:[2]
  • Cell lines: Human melanoma C8161 cells
  • Concentrations: 0, 80, 160, 320 μmol/L
  • Incubation Time: 48 h
  • Method: C8161 cells are grown in T75 flasks and treated with the indicated JH for 48 h. After the treatment, the cells are fixed with 70% ethanol overnight at 41℃, washed with PBS, and resuspended in 500 ml PBS with 100 mg/ml RNase. After incubation for 30 min at 37℃, 2.5 ml (10 mg/ml) PI solution is added and incubated on ice for another 30 min in the dark. The DNA contents of PI-stained cells are analyzed using flow cytometry.
Animal Research:[3]
  • Animal Models: 7-week-old male C57BL/6 mice
  • Dosages: 20 and 100 mg/kg
  • Administration: intragastric injections

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 338.38
Formula

C20H20NO4

CAS No. 3621-38-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC(=C(C=C4CC3)O)OC)OC

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Jatrorrhizine | Jatrorrhizine supplier | purchase Jatrorrhizine | Jatrorrhizine cost | Jatrorrhizine manufacturer | order Jatrorrhizine | Jatrorrhizine distributor