Catalog No.S3752 Synonyms: Ile

For research use only.

Isoleucine (Ile) is a non-polar, uncharged (at physiological pH), aliphatic amino acid that is used in the biosynthesis of proteins.

isoleucine Chemical Structure

CAS No. 73-32-5

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Biological Activity

Description Isoleucine (Ile) is a non-polar, uncharged (at physiological pH), aliphatic amino acid that is used in the biosynthesis of proteins.
In vitro

Isoleucine plays a major role in enhancing glucose consumption and utilization by up-regulating intestinal and muscular glucose transporters[1]. L-isoleucine could induce β-defensins’expressions in porcine intestinal epithelial cells[2].

In vivo Isoleucine is beneficial for mammary health, milk quality and embryo growth, enhances intestinal development, intestinal amino acid transportation and mucin production and participates in up-regulating innate and adaptive immune responses. Studies in mice have indicated that diet-induced obesity mice in an isoleucine treatment (final concentration of 2.5% isoleucine in drinking water) had almost 6% lower body weight gain and 49% less epididymal white adipose tissue mass compared with the control treatment, with higher levels of hepatic protein. Its deprivation induces fat mass loss in mice. Isoleucine could stimulate glucose uptake and participated in plasma glucose uptake in the rat[1].

Protocol (from reference)

Cell Research:[2]
  • Cell lines: IPEC-J2 cells
  • Concentrations: 0, 25, 50, 100, 250 and 500 μg/mL
  • Incubation Time: 12 h
  • Method: After cells are grown to 80 % confluence, they are starved for 12 h in serum and antibiotic-free DMEM/F12 medium. Following starvation, the cells are cultured for 12 h in the presence of 0, 25, 50, 100, 250 and 500 μg/mL L-isoleucine or 0, 25, 50, 100, 250 and 500 μmol/mL zinc sulfate that were used to increase the concentration of Zn2+ in the medium. Then, the cells are collected to examine the mRNA and protein expressions of pBD-1, 2 and 3, respectively.
  • (Only for Reference)

Solubility (25°C)

In vitro

Water 26 mg/mL
(198.21 mM)
DMSO Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 131.17


CAS No. 73-32-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC(C)C(C(=O)O)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05026047 Not yet recruiting Other: HPV self-sampling Early Detection of Oncogenic Papillomavirus Infection in Women Living With HIV Assistance Publique - Hôpitaux de Paris January 2022 Not Applicable
NCT05163860 Recruiting Other: Survey Family Relations|Nutrition Disorders|Health Behavior|Transgenderism St. Louis University December 27 2021 --
NCT05047757 Recruiting Dietary Supplement: Test meal with 15N labeled favabean Ileal Digestibility of Amino Acids Institut National de la Recherche Agronomique|University of Helsinki|AgroParisTech October 18 2021 Not Applicable
NCT04949607 Not yet recruiting Dietary Supplement: Inulin fructooligosaccharide Brain Injury Chronic|Brain Injuries Traumatic Centre for Neuro Skills|University of Texas July 1 2021 Not Applicable

(data from, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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