Name: isoleucine
Brand: Selleck Chemicals
SKU: S3752
Rating: 5 (1 reviews)

Isoleucine (Ile) is a non-polar, uncharged (at physiological pH), aliphatic amino acid that is used in the biosynthesis of proteins.

isoleucine Chemical Structure

CAS: 73-32-5

Purity & Quality Control

Batch: S375201 Water] 26 mg/mL] false] DMSO] Insoluble] false] ] ] false Purity: 98%

98

isoleucine Related Products

Biological Activity

Description	Isoleucine (Ile) is a non-polar, uncharged (at physiological pH), aliphatic amino acid that is used in the biosynthesis of proteins.

In vitro
In vitro	Isoleucine plays a major role in enhancing glucose consumption and utilization by up-regulating intestinal and muscular glucose transporters^[1]. L-isoleucine could induce β-defensins’expressions in porcine intestinal epithelial cells^[2].
Cell Research	Cell lines	IPEC-J2 cells
	Concentrations	0, 25, 50, 100, 250 and 500 μg/mL
	Incubation Time	12 h
	Method	After cells are grown to 80 % confluence, they are starved for 12 h in serum and antibiotic-free DMEM/F12 medium. Following starvation, the cells are cultured for 12 h in the presence of 0, 25, 50, 100, 250 and 500 μg/mL L-isoleucine or 0, 25, 50, 100, 250 and 500 μmol/mL zinc sulfate that were used to increase the concentration of Zn2+ in the medium. Then, the cells are collected to examine the mRNA and protein expressions of pBD-1, 2 and 3, respectively.

In Vivo
In vivo	Isoleucine is beneficial for mammary health, milk quality and embryo growth, enhances intestinal development, intestinal amino acid transportation and mucin production and participates in up-regulating innate and adaptive immune responses. Studies in mice have indicated that diet-induced obesity mice in an isoleucine treatment (final concentration of 2.5% isoleucine in drinking water) had almost 6% lower body weight gain and 49% less epididymal white adipose tissue mass compared with the control treatment, with higher levels of hepatic protein. Its deprivation induces fat mass loss in mice. Isoleucine could stimulate glucose uptake and participated in plasma glucose uptake in the rat^[1].

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06142513	Not yet recruiting	Obesity Hypoventilation Syndrome\|Obesity\|Hypoventilation\|Respiration Disorders\|Sleep Disorder; Breathing-Related	Istanbul University\|Istanbul University - Cerrahpasa (IUC)	December 1 2023	--
NCT05026047	Not yet recruiting	Papillomavirus Infection	Assistance Publique - Hôpitaux de Paris	May 2023	Not Applicable
NCT05812144	Recruiting	Type 1 Facioscapulohumeral Muscular Dystrophy	Centre Hospitalier Universitaire de Nice	May 23 2023	Not Applicable
NCT05726994	Recruiting	Cystic Fibrosis	Assistance Publique - Hôpitaux de Paris	February 6 2023	--

References

Chemical Information & Solubility

Molecular Weight	131.17	Formula	C₆H₁₃NO₂
CAS No.	73-32-5	SDF	Download isoleucine SDF Download isoleucine SDF
Smiles	CCC(C)C(C(=O)O)N
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

Water : 26 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

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% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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