Isoimperatorin

Catalog No.S3904

For research use only.

Isoimperatorin, a natural furanocoumarin that can be isolated from a variety of plant parts, has analgesic, antimicrobial, vascular relaxing and anticancer activities. Isoimperatorin is a methanolic extract of the roots of Angelica dahurica shows significant inhibitory effects on acetylcholinesterase (AChE) with the IC50 of 74.6 μM.

Isoimperatorin Chemical Structure

CAS No. 482-45-1

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Biological Activity

Description Isoimperatorin, a natural furanocoumarin that can be isolated from a variety of plant parts, has analgesic, antimicrobial, vascular relaxing and anticancer activities. Isoimperatorin is a methanolic extract of the roots of Angelica dahurica shows significant inhibitory effects on acetylcholinesterase (AChE) with the IC50 of 74.6 μM.
Targets
AChE [2]
(Cell-free assay)
74.6 μM
In vitro

Isoimperatorin significantly inhibits the proliferation of SGC-7901 cells in a dose- and time-dependent manner, and the half maximal inhibitory concentration is 18.75 µg/ml. The expression levels of the pro-apoptotic proteins, Bax, c-caspase-3 and c-caspase-9, are significantly increased and the expression levels of the anti-apoptotic proteins, Survivin and Bcl-2, are significantly reduced after the treatment[1].

In vivo

Isoimperatorin significantly inhibits the growth of SGC-7901 cell-induced tumor in vivo by increasing the expression levels of pro-apoptotic proteins (Bax, c-caspase-3 and c-caspase-9) and reducing the expression levels of anti-apoptotic proteins (Survivin and Bcl-2) without adverse effects on the increasing body weight of nude mice[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: SGC-7901 cells
  • Concentrations: 5, 10, 15, 20, 25, 30, 35 and 40 µg/ml
  • Incubation Time: 48 h
  • Method:

    The antiproliferative activity of isoimperatorin against SGC-7901 cells was evaluated using an MTT reduction assay. The SGC-7901 cells were seeded on 96-well culture plates with RPMI-1640 medium at a density of 5×103 cells/well. After 24 h of incubation at 37°C in 5% CO2/95% air, the cells were treated at different concentrations of isoimperatorin (5, 10, 15, 20, 25, 30, 35 and 40 µg/ml) and 0.05% DMSO (control) for 48 h to examine dose-dependency; or with isoimperatorin at a concentration of 20 µg/ml and 0.05% DMSO (control) for 12, 24, 36, 48, 60 and 72 h to examine time-dependent effects. Subsequently, 20 µl MTT (5 mg/ml) was separately added into each well, and the cells were cultured at 37°C in 5% CO2/95% air for another 3 h. Subsequently, 200 µl DMSO was separately added into each well and the optical density (OD) of the DMSO solution was measured at 570 nm using a microplate reader.

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: Sixteen female nude mice (5–6 weeks old) inoculated with SGC-7901 cells (tumor xenograft model)
  • Dosages: 10 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 54 mg/mL
(199.79 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 270.28
Formula

C16H14O4

CAS No. 482-45-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=CCOC1=C2C=CC(=O)OC2=CC3=C1C=CO3)C

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