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Hydrocortisone hemisuccinate Interleukins inhibitor

Name: Hydrocortisone hemisuccinate
Brand: Selleck Chemicals
SKU: S5972
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S5972

Hydrocortisone hemisuccinate (A-hydrocort, Hydrocortisone 21-hemisuccinate), a physiological glucocorticoid with anti-inflammatory properties, is an inhibitor of proinflammatory cytokine with IC50 of 6.7 μM and 21.4 μM for Interleukin-6 (IL-6) and IL-3, respectively.

Chemical Structure

Molecular Weight: 462.53

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Quality Control

Batch: Purity: 99.58%

99.58

Related Targets	PD-1/PD-L1 CXCR STING AhR Immunology & Inflammation related CD markers Anti-infection Antioxidant COX Histamine Receptor
Other Interleukins Inhibitors	SC144 Ossirene (AS101) Diacerein Apilimod mesylate Ac-YVAD-cmk EC330 Raleukin Diethyl phosphate Sodium Thiocyanate IQ 3

Solubility

In vitro
Batch:

DMSO : 93 mg/mL (201.06 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 93 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.650mg/ml (10.05mM)	Taking the 1 mL working solution as an example, add 50 μL of 93 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.770mg/ml (1.66mM)	Taking the 1 mL working solution as an example, add 50 μL of 15.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	462.53	Formula	C₂₅H₃₄O₈	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	2203-97-6	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	A-hydrocort, Hydrocortisone 21-hemisuccinate			SMILES	CC12CCC(=O)C=C1CCC3C4CCC(O)(C(=O)COC(=O)CCC(O)=O)C4(C)CC(O)C23

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	IL-6 (Cell-free assay) 6.7 μM IL-3 (Cell-free assay) 21.4 μM
References	[1] https://pubmed.ncbi.nlm.nih.gov/11411563/ [2] https://pubmed.ncbi.nlm.nih.gov/3497887/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-08-20)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06881927	RECRUITING	Sepsis and Septic Shock; Acute Respiratory Failure; Kidney Transplant Recipients; Immunosuppressive Agents	University Hospital, Strasbourg, France	2026-08-11	PHASE4
NCT01817153	TERMINATED	Septic Shock; Adult	Centre Hospitalier Régional d'Orléans	2013-11-05	PHASE2; PHASE3
NCT02768740	COMPLETED	Septic Shock	Association Niçoise de Réanimation Médicale	2008-04	PHASE4

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