Homatropine Methylbromide

Catalog No.S4024

For research use only.

Homatropine Methylbromide is muscarinic AChR antagonist, inhibits endothelial and smooth muscle muscarinic receptors of WKY-E and SHR-E with IC50 of 162.5 nM and 170.3 nM, respectively.

Homatropine Methylbromide Chemical Structure

CAS No. 80-49-9

Selleck's Homatropine Methylbromide has been cited by 1 Publication

Purity & Quality Control

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Biological Activity

Description Homatropine Methylbromide is muscarinic AChR antagonist, inhibits endothelial and smooth muscle muscarinic receptors of WKY-E and SHR-E with IC50 of 162.5 nM and 170.3 nM, respectively.
Targets
mAChR (WKY-E) [1] mAChR (SHR-E) [1]
162.5 nM 170.3 nM
In vitro

Homatropine (20 μM) alone produces a dose ratio of 259 in atrium from guinea-pigs. Homatropine (20 μM) produces a dose ratio of only 95.0 when combined with hexamethonium in atrium from guinea-pigs. [2] Homatropine has similar affinities for muscarinic receptors in stomach (pA2 = 7.13) and for those in atria mediating force (pA2 = 7.21) and rate (pA2 = 7.07) responses. [3]

In vivo Homatropine (20 mg/kg) prevents lethality in rats with dichlorvos poisoning with survive rate of 30% and time of death ranged between 4 and 12 minutes, while Homatropine (10 mg/kg) has no effect on preventing lethality. [4] Homatropine [14C]methylbromide administrated rectal achieves higher and rapid peak plasma concentrations than by the other routes in rats whether HMB-14C is administered in a water-soluble suppository base or in aqueous solution, retained 28% of the 14C has been excreted in the urine while 56% remained in the large intestine after 12 hours. Unlabelled Homatropine methylbromide, given in rectal suppositories to anaesthetized rats, causes prompt blockade of the effects of vagal stimulation on pulse rate and of intravenous acetylcholine on blood pressure. [5]

Protocol (from reference)

Animal Research:[4]
  • Animal Models: Sprague-Dawley rats
  • Dosages: 20 mg/kg
  • Administration: Intramuscular (IM) injections

Solubility (25°C)

In vitro

DMSO 74 mg/mL
(199.84 mM)
Water 74 mg/mL
(199.84 mM)
Ethanol 10 mg/mL
(27.0 mM)

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.

30 mg/mL

Chemical Information

Molecular Weight 370.28
Formula

C17H24NO3.Br

CAS No. 80-49-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C[N+]1(C2CCC1CC(C2)OC(=O)C(C3=CC=CC=C3)O)C.[Br-]

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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