Guanabenz

Catalog No.S4481 Synonyms: GBZ, GA, Wytensin, Wy-8678,BR-750

For research use only.

Guanabenz (GBZ, GA, Wytensin, Wy-8678,BR-750) is an orally active central alpha 2-adrenoceptor (α2 adrenergic receptor) agonist with antihypertensive action.

Guanabenz Chemical Structure

CAS No. 5051-62-7

Purity & Quality Control

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Biological Activity

Description Guanabenz (GBZ, GA, Wytensin, Wy-8678,BR-750) is an orally active central alpha 2-adrenoceptor (α2 adrenergic receptor) agonist with antihypertensive action.
Targets
α2 adrenergic receptor [1]
In vitro

Interleukin-6 (IL6), colony stimulating factor 2 (Csf2), and cyclooxygenase-2 (Cox2) are downregulated by guanabenz-driven phosphorylation of eukaryotic translation initiation factor 2α (eIF2α). Although expression of IL1β and Tumor Necrosis Factor-α (TNFα) was suppressed by guanabenz, their downregulation was not directly mediated by eIF2α signaling.[2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: RAW264.7 macrophages, primary macrophages, Jurkat T lymphocytes and HMC-1.1 mast cells
  • Concentrations: 5, 10, and 20 µM
  • Incubation Time: 2 days
  • Method:

    RAW264.7 macrophages and primary macrophages were cultured in αMEM with 10% FBS and antibiotics. Mouse bone marrow cells were grown with 10 ng/mL macrophage colony-stimulating factor for three days, and the surface-attached cells were used as primary macrophages. Jurkat T lymphocytes and HMC-1.1 mast cells were cultured in RPMI 1640 and IMDM with 1-thioglycerol, respectively. RAW264.7 cells and primary macrophages were activated by 0.1 or 1 µg/mL lipopolysaccharide (LPS), while Jurkat cells and HMC-1.1 cells were activated by 100 nM phorbol myristate acetate (PMA) and 1 µM ionomycin. Of note, the MTT assay was conducted by RAW264.7 cells for evaluating cytotoxicity.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 231.08
Formula
C8H8Cl2N4
CAS No. 5051-62-7
Storage 3 years -20°C powder
2 years -80°C in solvent

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05465161 Not yet recruiting Device: Sting Mark Fiducial Marker Cancer Centre hospitalier de l''Université de Montréal (CHUM) October 1 2022 Not Applicable
NCT05446376 Not yet recruiting Drug: 68Ga-citrate PET/CT Infective Endocarditis University Hospital Bordeaux September 2022 Phase 2
NCT05321862 Not yet recruiting Drug: [68Ga]Ga-PentixaFor Multiple Myeloma Nantes University Hospital September 2022 Phase 2
NCT05446324 Not yet recruiting Drug: Gallium-68-tilmanocept Endometrial Cancer UMC Utrecht September 1 2022 Phase 3
NCT04243941 Not yet recruiting Drug: 68Ga-HBED-CC-PSMA Prostate Cancer UNC Lineberger Comprehensive Cancer Center September 15 2022 Phase 2
NCT04480619 Not yet recruiting Diagnostic Test: Ga-68-PEG-Αvβ3-IAC PET/CT Diagnosis and Clinical Management of Angiogenic Breast Cancer Advanced Imaging Projects LLC|All India Institute of Medical Sciences New Delhi|Post Graduate Institute of Medical Education and Research|University of Witwatersrand South Africa|US Department of Veterans Affairs August 30 2022 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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