Guanabenz

Catalog No.S4481 Batch:S448101

Technical Data

Formula

C₈H₈Cl₂N₄

Molecular Weight

231.08

CAS No.

5051-62-7

Solubility (25°C)*

In vitro

Ethanol

15 mg/mL (64.91 mM)

DMSO

12 mg/mL (51.93 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution

5%DMSO 1 Corn oil

0.6mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 12 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Guanabenz (GBZ, GA, Wytensin, Wy-8678,BR-750) is an orally active central alpha 2-adrenoceptor (α2 adrenergic receptor) agonist with antihypertensive action.

Targets

α2 adrenergic receptor ^[1]

In vitro

Interleukin-6 (IL6), colony stimulating factor 2 (Csf2), and cyclooxygenase-2 (Cox2) are downregulated by guanabenz-driven phosphorylation of eukaryotic translation initiation factor 2α (eIF2α). Although expression of IL1β and Tumor Necrosis Factor-α (TNFα) was suppressed by guanabenz, their downregulation was not directly mediated by eIF2α signaling.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines RAW264.7 macrophages, primary macrophages, Jurkat T lymphocytes and HMC-1.1 mast cells Concentrations 5, 10, and 20 µM Incubation Time 2 days Method RAW264.7 macrophages and primary macrophages were cultured in αMEM with 10% FBS and antibiotics. Mouse bone marrow cells were grown with 10 ng/mL macrophage colony-stimulating factor for three days, and the surface-attached cells were used as primary macrophages. Jurkat T lymphocytes and HMC-1.1 mast cells were cultured in RPMI 1640 and IMDM with 1-thioglycerol, respectively. RAW264.7 cells and primary macrophages were activated by 0.1 or 1 µg/mL lipopolysaccharide (LPS), while Jurkat cells and HMC-1.1 cells were activated by 100 nM phorbol myristate acetate (PMA) and 1 µM ionomycin. Of note, the MTT assay was conducted by RAW264.7 cells for evaluating cytotoxicity.

Cell Assay:^{^[2]}

Cell lines
RAW264.7 macrophages, primary macrophages, Jurkat T lymphocytes and HMC-1.1 mast cells
Concentrations
5, 10, and 20 µM
Incubation Time
2 days
Method
RAW264.7 macrophages and primary macrophages were cultured in αMEM with 10% FBS and antibiotics. Mouse bone marrow cells were grown with 10 ng/mL macrophage colony-stimulating factor for three days, and the surface-attached cells were used as primary macrophages. Jurkat T lymphocytes and HMC-1.1 mast cells were cultured in RPMI 1640 and IMDM with 1-thioglycerol, respectively. RAW264.7 cells and primary macrophages were activated by 0.1 or 1 µg/mL lipopolysaccharide (LPS), while Jurkat cells and HMC-1.1 cells were activated by 100 nM phorbol myristate acetate (PMA) and 1 µM ionomycin. Of note, the MTT assay was conducted by RAW264.7 cells for evaluating cytotoxicity.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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