Home Metabolism Casein Kinase inhibitor Ellagic Acid hydrate

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Ellagic Acid hydrate Casein Kinase inhibitor

Cat.No.S5516

Ellagic acid is a potent inhibitor of protein kinase CK2 with IC50s of 0.04, 2.9 and 3.5 μM for CK2, Lyn and PKA respectively. It shows potent antioxidant, anti-mutagenic and antidepressant properties.
Ellagic Acid hydrate Casein Kinase inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 320.21

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Quality Control

Batch: S551601 DMSO]2 mg/mL]false]]]false]]]false Purity: 98%
98

Solubility

In vitro
Batch:

DMSO : 2 mg/mL (6.24 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 320.21 Formula

C14H6O8.H2O

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 314041-08-2 -- Storage of Stock Solutions

Synonyms N/A Smiles C1=C2C3=C(C(=C1O)O)OC(=O)C4=CC(=C(C(=C43)OC2=O)O)O.O

Mechanism of Action

Targets/IC50/Ki
CK2
(Cell-free assay)
0.04 μM
Lyn
(Cell-free assay)
2.9 μM
PKA
(Cell-free assay)
3.5 μM
In vitro
Ellagic acid (EA) is able to inhibit the growth of several cancer cells. EA inhibited cell proliferation in a dose- and time-dependent manner by arresting both cell lines at the G1 phase of the cell cycle, which were from elevating p53 and Cip1/p21 and decreasing cyclin D1 and E levels. EA also induced caspase-3-mediated apoptosis by increasing the Bax : Bcl-2 ratio and restored anoikis in ES-2 and PA-1 cells. It is well known to have a free radical scavenging activity. EA reversed epithelial to mesenchymal transition by up-regulating E-cadherin and down-regulating Vimentin.
In vivo
A 90-day subchronic toxicity study further demonstrated that orally feeding EA (9.4, 19.1, 39.1 g/kg b.w., resp.) could not induce mortality or treatment-related clinical signs throughout the experimental period on F344 rats, indicating the low toxicity of EA to mammalians. Furthermore, EA exhibits potent anticancer and anticarcinogenesis activities towards breast, colorectal, oral, prostate, pancreatic, bladder, neuroblastoma, melanoma, and lymphoma cells. Treatment of PANC-1 xenografted mice with EA resulted in significant inhibition in tumor growth and prolong mice survival rate.
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