Ellagic Acid hydrate

Catalog No.S5516

For research use only.

Ellagic acid is a potent inhibitor of protein kinase CK2 with IC50s of 0.04, 2.9 and 3.5 μM for CK2, Lyn and PKA respectively. It shows potent antioxidant, anti-mutagenic and antidepressant properties.

Ellagic Acid hydrate Chemical Structure

CAS No. 314041-08-2

Purity & Quality Control

Choose Selective Casein Kinase Inhibitors

Biological Activity

Description Ellagic acid is a potent inhibitor of protein kinase CK2 with IC50s of 0.04, 2.9 and 3.5 μM for CK2, Lyn and PKA respectively. It shows potent antioxidant, anti-mutagenic and antidepressant properties.
Targets
CK2 [1]
(Cell-free assay)
Lyn [1]
(Cell-free assay)
PKA [1]
(Cell-free assay)
0.04 μM 2.9 μM 3.5 μM
In vitro

Ellagic acid (EA) is able to inhibit the growth of several cancer cells. EA inhibited cell proliferation in a dose- and time-dependent manner by arresting both cell lines at the G1 phase of the cell cycle, which were from elevating p53 and Cip1/p21 and decreasing cyclin D1 and E levels. EA also induced caspase-3-mediated apoptosis by increasing the Bax : Bcl-2 ratio and restored anoikis in ES-2 and PA-1 cells. It is well known to have a free radical scavenging activity[1]. EA reversed epithelial to mesenchymal transition by up-regulating E-cadherin and down-regulating Vimentin[2].

In vivo A 90-day subchronic toxicity study further demonstrated that orally feeding EA (9.4, 19.1, 39.1 g/kg b.w., resp.) could not induce mortality or treatment-related clinical signs throughout the experimental period on F344 rats, indicating the low toxicity of EA to mammalians. Furthermore, EA exhibits potent anticancer and anticarcinogenesis activities towards breast, colorectal, oral, prostate, pancreatic, bladder, neuroblastoma, melanoma, and lymphoma cells[1]. Treatment of PANC-1 xenografted mice with EA resulted in significant inhibition in tumor growth and prolong mice survival rate[2].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Ovarian carcinoma ES-2 and PA-1 cells
  • Concentrations: 10~100 μM
  • Incubation Time: 12, 24, 48 h
  • Method:

    Ovarian carcinoma cell lines were plated at 100,000 cells in six-well tissue culture dishes. After 18 h of culture, cells were treated with different concentrations of DMSO-dissolved EA (0, 10, 25, 50, 75, or 100 μM) or chemotherapeutic drugs (doxorubicin, paraplatin, and paclitaxel) or a combination of both drugs. At the various time points, cells were collected by trypsinization and stained with trypan blue, and the cell number in suspension was counted in duplicate using a hemocytometer.

  • (Only for Reference)
Animal Research:

[2]

  • Animal Models: PANC-1 xenografts (Balb C nude mice, 4-6 weeks old)
  • Dosages: 0, 10 mg/kg, 20 mg/kg and 40 mg/kg
  • Administration: oral gavage
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 2 mg/mL
(6.24 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 320.21
Formula

C14H6O8.H2O

CAS No. 314041-08-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=C2C3=C(C(=C1O)O)OC(=O)C4=CC(=C(C(=C43)OC2=O)O)O.O

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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