Dehydroevodiamine hydrochloride

Catalog No.S3820

For research use only.

Dehydroevodiamine (DHE), a major phytochemical in fruits of Evodia rutaecarpa, can inhibit AChE. It has hypotensive and neuroprotective effects and modulates nitric oxide production.

Dehydroevodiamine hydrochloride Chemical Structure

CAS No. 111664-82-5

Purity & Quality Control

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Biological Activity

Description Dehydroevodiamine (DHE), a major phytochemical in fruits of Evodia rutaecarpa, can inhibit AChE. It has hypotensive and neuroprotective effects and modulates nitric oxide production.
Targets
AChE [2]
()
In vitro

Dehydroevodiamine·HCl (DHED) treatment significantly recovers the decreases in the levels of neural cell adhesion molecule (NCAM) proteins caused by stress and the decreases in cell viability[1]. It decreases intracellular calcium levels in primary cortical neurons[2].

In vivo Dehydroevodiamine·HCl (DHED) can improve stress-induced memory impairments and depression-like behaviors[1]. DHED significantly improves learning and memory in the Aβ1-42-infused rat model. Antiamnestic effects of DHED occur through an improvement of learning and memory and an inhibition of neuronal dysfunction in a scopolamine-induced amnesic rat model. Furthermore, Dehydroevodiamine·HCl (DHED) has hypotensive and neuroprotective effects and modulates nitric oxide production[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: primary cortical neurons
  • Concentrations: 0.5 and 1 µM
  • Incubation Time: 4 h
  • Method: Cells are cultured in a specified medium for neurons (neurobasal medium supplemented with B27 and penicillin-streptomycin (100 U/ml/100 mg/ml) at 37℃ and 5% CO2 and plated at a density of 5×104 cells/well on Deckgläser cover glasses coated with PLL (1 mg/ml) in the 12 well plates. Experiments are performed on 10-day cultures. After DHED (0.5 and 1 µM) and fluoxetine (0.5 µM) treatment for 4 hr, staurosporin (1 µM) are added for 1 hr.
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: male Wistar rats
  • Dosages: 10 mg/kg/day
  • Administration: p.o.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 37 mg/mL
(109.53 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 337.8
Formula

C19H15N3O.HCl

CAS No. 111664-82-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN1C2=CC=CC=C2C(=O)N3C1=C4C(=C5C=CC=CC5=N4)CC3.Cl

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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