Cyclic Pifithrin-α hydrobromide

Catalog No.S5791

For research use only.

Cyclic Pifithrin-α hydrobromide is the hydrobromide salt form of Cyclic Pifithrin-α, which is a transcriptional inhibitor of p53.

Cyclic Pifithrin-α hydrobromide  Chemical Structure

CAS No. 511296-88-1

Selleck's Cyclic Pifithrin-α hydrobromide has been cited by 1 Publication

Purity & Quality Control

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Biological Activity

Description Cyclic Pifithrin-α hydrobromide is the hydrobromide salt form of Cyclic Pifithrin-α, which is a transcriptional inhibitor of p53.
Targets
p53 [1]
()

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: H460 and IGROV-1 cell lines
  • Concentrations: 10 μM and 20 μM
  • Incubation Time: 24 h
  • Method:

    H460 and IGROV-1 cell lines were treated with either PFT or paclitaxel alone or with their combination for 24 hours. After washing with PBS, the cells were fixed in 2% paraformaldehyde for 30 minutes and permeabilized with ice-cold methanol for 20 minutes at −20°C. After blocking with PBA (PBS containing 1% bovine serum albumin), anti-p53 antibody was incubated for 1 hour at room temperature followed by incubation with Alexa Fluor 488 goat antimouse IgG secondary antibody for 1 hour. Images were observed and analyzed by confocal microscopy.

Solubility (25°C)

In vitro

DMSO 27 mg/mL
(77.29 mM)
Ethanol 3 mg/mL
(8.58 mM)
Water Insoluble

Chemical Information

Molecular Weight 349.29
Formula

C16H17BrN2S

CAS No. 511296-88-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=CC=C(C=C1)C2=CN3C4=C(CCCC4)SC3=N2.Br

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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