CCCP

Catalog No.S6494 Batch:S649404

Technical Data

Formula	C₉H₅ClN₄
Molecular Weight	204.62	CAS No.	555-60-2
Solubility (25°C)*	In vitro	DMSO	41 mg/mL (200.37 mM)
		Ethanol	14 mg/mL (68.41 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	CCCP (Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone), an oxidative phosphorylation inhibitor, is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), the protonophore, can inhibits STING-mediated IFN-β production via disrupting mitochondrial membrane potential (MMP).
In vitro	CCCP, a drug perturbing adenosine triphosphate synthesis by transporting protons across the mitochondrial inner membrane, reduces the spheroid-forming cell (SFC) frequency, indicating sensitivity of stem-like cells towards oxidative phosphorylation (OXPHOS) inhibition.^[1]
In vivo	Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP), an oxidative phosphorylation (OXPHOS) uncoupler, kills bacterial cells and prevents forming of biofilm.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines cells freshly isolated from patient material or PDXs (patient-derived xenografts) Concentrations 25 µM Incubation Time 4 h Method 5× 10⁵ tumor spheroid cells are seeded into two wells of 6-well ultra-low attachment plates. After seven days, 25 µM CCCP or DMSO are added and cells are incubated for 4 h at 37 ℃. Cells are dissociated, stained with 200 nM TOTO-3 in PBS, and prepared for cell sorting as described. Living cells are sorted into 96-well ultra-low attachment plates containing 100 µL of fresh culture medium supplemented with 100 µg/mL streptomycin and 100 U/mL penicillin per well. Limiting dilution and determination of SFC frequency are performed.
Animal Study:^{^[2]}	Animal Models Female Balb/c mice Dosages 3 mg/kg Administration i.v.

Cell Assay:^{^[1]}

Cell lines
cells freshly isolated from patient material or PDXs (patient-derived xenografts)
Concentrations
25 µM
Incubation Time
4 h
Method
5× 10⁵ tumor spheroid cells are seeded into two wells of 6-well ultra-low attachment plates. After seven days, 25 µM CCCP or DMSO are added and cells are incubated for 4 h at 37 ℃. Cells are dissociated, stained with 200 nM TOTO-3 in PBS, and prepared for cell sorting as described. Living cells are sorted into 96-well ultra-low attachment plates containing 100 µL of fresh culture medium supplemented with 100 µg/mL streptomycin and 100 U/mL penicillin per well. Limiting dilution and determination of SFC frequency are performed.

Animal Study:^{^[2]}

Animal Models
Female Balb/c mice
Dosages
3 mg/kg
Administration
i.v.

References

Selleck's CCCP has been cited by 21 publications

TBC1D23 mediates Golgi-specific LKB1 signaling [ Nat Commun, 2024, 15(1):1785]	PubMed: 38413626
Gonococcal OMV-delivered PorB induces epithelial cell mitophagy [ Nat Commun, 2024, 15(1):1669]	PubMed: 38396029
Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis [ Redox Biol, 2024, 69:103002]	PubMed: 38142583
Fut2 Deficiency Promotes Intestinal Stem Cell Aging by Damaging Mitochondrial Functions via Down-Regulating α1,2-Fucosylation of Asah2 and Npc1 [ Research (Wash D C), 2024, 7:0343]	PubMed: 38550777
PDGF-BB accelerates TSCC via fibroblast lactates limiting miR-26a-5p and boosting mitophagy [ Cancer Cell Int, 2024, 24(1):5]	PubMed: 38169376
TRIM28 promotes porcine epidemic diarrhea virus replication by mitophagy-mediated inhibition of the JAK-STAT1 pathway [ Int J Biol Macromol, 2023, 10.1016/j.ijbiomac.2023.127722]	PubMed: 37907173
A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis [ Cell Res, 2022, 32(2):119-138]	PubMed: 34561617
Novel roles of RTN4 and CLIMP-63 in regulating mitochondrial structure, bioenergetics and apoptosis [ Cell Death Dis, 2022, 13(5):436]	PubMed: 35508606
Neospora caninum Evades Immunity via Inducing Host Cell Mitophagy to Inhibit Production of Proinflammatory Cytokines in a ROS-Dependent Manner [ Front Immunol, 2022, 13:827004]	PubMed: 35355995
Characterization of immune features and immunotherapy response in subtypes of hepatocellular carcinoma based on mitophagy [ Front Immunol, 2022, 13:966167]	PubMed: 36304466

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SHIPPING AND STORAGE
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