Carvacrol

Catalog No.S3788 Synonyms: cymophenol

For research use only.

Carvacrol (Cymophenol), monoterpenic phenol isomeric with thymol, has diverse activities such as antimicrobial, antitumor, an-timutagenic, antigenotoxic, analgesic, antispasmodic, anti-inflammatory, angiogenic, antiparasitic, antiplatelet, AChE inhibitory, antielastase, insecticidal, antihepatotoxic and hepatoprotective activities.

Carvacrol Chemical Structure

CAS No. 499-75-2

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Biological Activity

Description Carvacrol (Cymophenol), monoterpenic phenol isomeric with thymol, has diverse activities such as antimicrobial, antitumor, an-timutagenic, antigenotoxic, analgesic, antispasmodic, anti-inflammatory, angiogenic, antiparasitic, antiplatelet, AChE inhibitory, antielastase, insecticidal, antihepatotoxic and hepatoprotective activities.
In vitro

Carvacrol (CVC) possesses weak antioxidant and cytotoxic activity in cultured primary rat neurons. In addition, Carvacrol has weak antioxidant properties and little anticancer potentials in rat N2a neuroblastoma cell line[1]. Carvacrol is a novel inhibitor of transient receptor potential (TRP) channels in drosophila and mammalian. In human hepatoma HepG2 cells, Carvacrol induces cell apoptosis by selectively decreasing phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and P38; In human macrophage-like U937 cells, in response to lipopolysaccharide treatment, Carvacrol activates peroxisome proliferator-activated receptors (PPAR alpha and gamma) and suppresses cyclooxygenase-2 (COX-2) mRNA and protein expression[2].

In vivo Carvacrol has the ability to protect liver against ischemia/reperfusion injury in rats. In the central nervous system, Carvacrol is regarded as a potential drug for Alzheimer's disease due to its inhibitory effect on acetylcholinesterase (AChE) activity by using phenolic hydroxyl group of carvacrol to bind to AChE and leading to a loss of function of AChE. In addition, Carvacrol is found to have an antidepressant-like effect in mice by affecting the dopaminergic system. Dietary carvacrol supplementation prevents high fat diet-induced obesity by modulating gene expressions that lead to adipogenesis and inflammation. Carvacrol crosses the blood-brain barrier easily and rapidly[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: cultured primary rat neurons and N2a neuroblastoma cells
  • Concentrations: 10, 25, 50, 100, 200 and 400 mg/L
  • Incubation Time: 24 h
  • Method: The cells are seeded in 48-well plates. Cells are incubated at 37 °C in a humidified 5 % CO2/95 % air mixture and treated with carvacrol at different concentrations (10, 25, 50, 100, 200 and 400 mg/L) for 24 h. MTT substrate solution is used. Briefly, MTT is added to the cell cultures for 3 h. Formed formazan crystals are dissolved in dimethyl sulfoxide (DMSO), MTT is added to the cell cultures for 3 h. Formed formazan crystals are dissolved in dimethyl sulfoxide (DMSO).
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: Male ICR mice
  • Dosages: 50 mg/kg
  • Administration: i.c.v.
  • (Only for Reference)

Chemical Information

Molecular Weight 150.22
Formula

C10H14O

Density 0.976 g/mL at 20 °C
CAS No. 499-75-2
Storage 2 years -20°C liquid
Smiles CC1=C(C=C(C=C1)C(C)C)O

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