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Acotiamide hydrochloride AChR inhibitor

Name: Acotiamide hydrochloride
Brand: Selleck Chemicals
SKU: S4838
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S4838

Acotiamide Hydrochloride (YM-443, Z-338) is the hydrochloride salt form of acotiamide, a prokinetic agent with gastrointestinal (GI) motility-enhancing activity. It is a new orally active selective acetylcholinesterase inhibitor.

Chemical Structure

Molecular Weight: 541.06

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Quality Control

Batch: S483801 DMSO]100 mg/mL]false]Ethanol]20 mg/mL]false]Water]5 mg/mL]false Purity: 99.80%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS/MSDS
Datasheet

99.80

Related Targets	Adrenergic Receptor 5-HT Receptor COX Calcium Channel Histamine Receptor Dopamine Receptor GABA Receptor TRP Channel Cholinesterase (ChE) GluR
Other AChR Inhibitors	PNU-120596 Benzethonium Chloride Arecoline HBr Otilonium Bromide Palmatine Lycorine Nitenpyram PNU 282987 HCl Chelidonine Hyoscyamine

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (184.82 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 20 mg/mL

Water : 5 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5mg/ml (9.24mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.71mg/ml (1.31mM)	Taking the 1 mL working solution as an example, add 50 μL of 14.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	541.06	Formula	C₂₁H₃₀N₄O₅S.HCl.3H₂O	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	773092-05-0	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	YM-443 HCl, Z-338 HCl			SMILES	CC(C)N(CCNC(=O)C1=CSC(=N1)NC(=O)C2=CC(=C(C=C2O)OC)OC)C(C)C.O.O.O.Cl

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro	Acotiamide inhibits AChE with half maximal inhibitory concentrations (IC50) of 3.0, 2.3 and 1.2 μM for human recombinant, and rat and canine gastric AChEs, respectively.
In vivo	Acotiamide is concentrated into the stomach tissue by carrier-mediated uptake processes, which may account for the selective action of acotiamide for gastric smooth muscle but not for skeletal muscle and central nervous system in rats.
References	[1] https://pubmed.ncbi.nlm.nih.gov/26682939/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2025-09-23)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07174297	NOT_YET_RECRUITING	Postprandial Distress Syndrome	Getz Pharma	2025-10-30	PHASE3
NCT07449689	NOT_YET_RECRUITING	Functioanl Dyspepsia; Post Prandial Distress Syndrome	Aga Khan University	2026-04	PHASE4
NCT04526119	COMPLETED	Functional Dyspepsia	Zeria Pharmaceutical	2021-02-22	PHASE3
NCT04697641	COMPLETED	Dyspepsia; Helicobacter Pylori Infection	Sir Ganga Ram Hospital	2017-09-01
NCT03402984	COMPLETED	Healthy	Universitaire Ziekenhuizen KU Leuven	2017-04-01

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