Acetylcholine iodide

Catalog No.S4718 Synonyms: Acetylcolina

For research use only.

Acetylcholine iodide (Acetylcolina) is a neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.

Acetylcholine iodide Chemical Structure

CAS No. 2260-50-6

Selleck's Acetylcholine iodide has been cited by 1 Publication

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Biological Activity

Description Acetylcholine iodide (Acetylcolina) is a neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.
In vitro

Acetylcholine iodide (Ach) stimulated SBC3 cell proliferation, adhesion and migration toward fibronectin (Fn) in a dose-dependent manner[1]. ACh ameliorates TNF-α-induced calpain activation by decreasing p38-MAPK phosphorylation and enhancing calpastatin expression. It elicits an anti-apoptotic effect through the activation of the muscarinic ACh receptor (MAChR) and the activation of anti-oxidant systems. ACh increases cell viability and decreases TNF-α-induced apoptosis in H9c2 cells[2].

In vivo Acetylcholine can inhibit pro-inflammatory cytokine release and protect against cardiomyocyte injury[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: The SBC3, human SCLC cell line
  • Concentrations: 1, 10, 100 μM
  • Incubation Time: 24, 48 or 72 h
  • Method: The SBC3 cells are seeded at 2000 cells/well in treated 96-well plates maintained with RPMI1640 medium containing 10% (v/v) FCS for 24 h. The medium is removed and replaced with 100 μl RPMI1640 containing 1% (v/v) ITS. The cells are incubated in serum free medium for 24 h to synchronize the cell cycle. Then another 100 μl serum-free medium containing the agonist/antagonist under investigation is added. The antagonist is added 30 min before Ach(Acetylcholine iodide). At this time, the cells in a duplicate plate are given 100 μl serum-free medium and a MTT assay is used to determine live cell numbers before treatment with the agonist/antagonist as measured by the absorbance (OD) at 550 nm/650 nm. After 24, 48 or 72 h culture, the remaining cells are assayed to yield post-agonist/antagonist cell numbers using the same method. All the experiments are performed in triplicate.
Animal Research:[3]
  • Animal Models: Female albino rats
  • Dosages: 25 or 50 mg/kg
  • Administration: s.c

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 273.11
Formula

C7H16NO2.I

CAS No. 2260-50-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=O)OCC[N+](C)(C)C.[I-]

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